本文選題：病毒性腦炎 + 急性期��； 參考：《山西醫(yī)科大學》2017年碩士論文

【摘要】：目的:探討病毒性腦炎急性期出現(xiàn)癲癇發(fā)作與血清及腦脊液中白細胞介素-1β(IL-1β)、白細胞介素-6(IL-6)及腫瘤壞死因子-α(TNF-α)水平之間的關系。方法:1.研究對象收集2016年3月-2017年1月就診于山西醫(yī)科大學第一附屬醫(yī)院神經(jīng)內(nèi)科住院的30例病毒性腦炎急性期患者,根據(jù)有無癲癇發(fā)作將其分為兩組,A組為病毒性腦炎急性期不出現(xiàn)的癲癇發(fā)作患者,B組為病毒性腦炎急性期出現(xiàn)的癲癇發(fā)作患者。選取非病毒性腦炎性癲癇患者10例為C組,D組為10例需脊髓麻醉并排除感染及炎癥的非癲癇患者(對照組)。2.標本采集在征得患者或家屬的同意后,采集各組患者的血液及腦脊液,3000rpm離心10分鐘后,分別提取上清液于1.5ml于EP管中,置于-80℃冰箱中凍存?zhèn)溆谩?.血清及腦脊液IL-1β、IL-6及TNF-α濃度水平測定采用夾心法酶聯(lián)免疫吸附試驗(enzyme-linked immunosorbent assay ELISA)方法測定血清及腦脊液IL-1β、IL-6及TNF-α濃度水平。4.數(shù)據(jù)處理:用SPSS19.0統(tǒng)計軟件進行統(tǒng)計學分析,所有數(shù)據(jù)用均數(shù)±標準差((?)±s)表示,各組數(shù)據(jù)采用單因素方差分析,組間比較采用用LSD方法,以P0.05表示有統(tǒng)計學意義。結果:1.各組血清中IL-1β、IL-6及TNF-α水平比較與對照組相比,A組與B組血清中IL-1β、IL-6及TNF-α水平明顯升高,差異有統(tǒng)計學意義(P0.05);C組血清中IL-1β濃度水平較對照組略高,差別無統(tǒng)計學意義(P0.05),而C組血清中IL-6和TNF-α水平較對照組略低,差別無統(tǒng)計學意義(P0.05)。與C組相比,A組與B組血清中IL-1β、IL-6及TNF-α水平明顯升高,差別有統(tǒng)計學意義(P0.05)。與A組相比,B組血清中IL-1β、IL-6及TNF-α水平顯著升高,差別具有統(tǒng)計學意義(P0.05),提示病毒性腦炎急性期血清中IL-1β、IL-6及TNF-α水平與急性期癲癇發(fā)作有關。2.各組腦脊液中IL-1β、IL-6及TNF-α水平比較與對照組相比,A組與B組腦脊液中IL-1β、IL-6及TNF-α水平明顯升高,差別具有統(tǒng)計學意義(P0.05);C組腦脊液中IL-6水平較對照組略高,差別無統(tǒng)計學意義(P0.05),而C組腦脊液中IL-1β和TNF-α水平較對照組略低,差別無統(tǒng)計學意義(P0.05)。與C組相比,A組與B組腦脊液中IL-1β、IL-6及TNF-α水平明顯升高,差別有統(tǒng)計學意義(P0.05);與A組相比,B組腦脊液中IL-1β、IL-6及TNF-α水平顯著升高,差別具有統(tǒng)計學意義(P0.05),提示病毒性腦炎急性期腦脊液中IL-1β、IL-6及TNF-α水平與急性期癲癇發(fā)作有關。結論:1.病毒性腦炎急性期癲癇發(fā)作患者血清中IL-1β、IL-6及TNF-α水平明顯高于病毒性腦炎急性期無癲癇發(fā)作患者血清中IL-1β、IL-6及TNF-α水平,提示病毒性腦炎急性期癲癇發(fā)作與血清中IL-1β、IL-6及TNF-α水平升高有關。2.病毒性腦炎急性期癲癇發(fā)作患者腦脊液中IL-1β、IL-6及TNF-α水平顯著高于病毒性腦炎急性期無癲癇發(fā)作患者腦脊液中IL-1β、IL-6及TNF-α水平,提示病毒性腦炎急性期癲癇發(fā)作與腦脊液中IL-1β、IL-6及TNF-α水平升高有關。
[Abstract]:Objective: To investigate the relationship between epileptic seizures in acute stage of viral encephalitis with interleukin -1 beta (IL-1 beta), interleukin -6 (IL-6) and tumor necrosis factor - alpha (TNF- alpha) in serum and cerebrospinal fluid. Methods: 1. subjects were collected in the First Affiliated Hospital of Shanxi Medical University in January March 2016 and were hospitalized in the Department of Neurology. The 30 patients with acute viral encephalitis were divided into two groups according to the seizures, the A group was the non epileptic seizures in the acute stage of viral encephalitis, and the B group was the epileptic seizures in the acute stage of viral encephalitis. 10 cases of non viral encephalitis epilepsies were selected as C group, and 10 patients in group D needed spinal anesthesia and excluded infection. The.2. specimen of the non epileptic patients (control group) and the non epileptic patients (control group) collected the blood and cerebrospinal fluid of each group after receiving the consent of the patients or family members. After 3000rpm centrifugation for 10 minutes, the supernatant was extracted from 1.5ml in the EP tube, and the concentration level of.3. serum and cerebrospinal fluid IL-1 beta, IL-6 and TNF- alpha concentration was measured in -80 centigrade refrigerator. Enzyme-linked immunosorbent assay ELISA method was used to determine the serum and cerebrospinal fluid IL-1 beta, IL-6 and TNF- alpha concentration level.4. data processing: statistical analysis was performed with SPSS19.0 statistical software. All data were expressed with mean mean + standard deviation ((?) + s). The data of each group were analyzed by single factor variance and compared between groups. In 1. groups, the levels of IL-1 beta, IL-6 and TNF- alpha in serum were compared with those in the control group, and the levels of IL-1 beta, IL-6 and TNF- a in the serum of A and B groups were significantly higher than those in the control group. The difference was statistically significant (P0.05), and there was no significant difference in the level of serum beta concentration in the serum of the C group, and there was no significant difference in serum levels of IL-1 beta, IL-6 and TNF- a in the serum of the B group. The levels of serum IL-6 and TNF- alpha in serum were slightly lower than those in the control group (P0.05). Compared with the C group, the levels of IL-1 beta, IL-6 and TNF- alpha in the serum of A and B groups increased significantly (P0.05). Compared with the A group, the levels of the serum in the B group were significantly higher than those in the A group, and the difference was statistically significant. The levels of IL-1 beta, IL-6 and TNF- alpha in the serum of acute toxic encephalitis were compared with the control group, the levels of IL-1 beta, IL-6 and TNF- alpha in the cerebrospinal fluid of.2. groups were compared with those in the control group. The levels of IL-1 beta, IL-6 and TNF- alpha in the cerebrospinal fluid of A and B groups were significantly higher than those in the control group, and the difference was statistically significant. The difference was not statistically significant (P0.05), but the level of IL-1 beta and TNF- alpha in the cerebrospinal fluid of C group was slightly lower than that of the control group (P0.05). Compared with the C group, the level of IL-1 beta, IL-6 and TNF- alpha in the cerebrospinal fluid of the A group and B group was significantly higher than that in the B group, and the difference was statistically significant (P0.05), compared with the group of cerebrospinal fluid, the level of the cerebrospinal fluid in the cerebrospinal fluid was significantly higher. The difference has statistical significance (P0.05), suggesting that the levels of IL-1 beta, IL-6 and TNF- alpha in cerebrospinal fluid in acute phase of viral encephalitis are related to acute epileptic seizures. Conclusion: the levels of IL-1 beta, IL-6 and TNF- a in the serum of 1. viral encephalitis patients with acute phase epilepsy are significantly higher than IL-1 beta in serum of patients with acute toxic encephalitis without epileptic seizures, IL The level of -6 and TNF- alpha suggests that the level of IL-1 beta, IL-6 and TNF- alpha in serum in acute phase of viral encephalitis is higher than that in the cerebrospinal fluid of patients with acute phase of.2. viral encephalitis. The level of IL-1 beta, IL-6 and TNF- is significantly higher than that of IL-1 beta, IL-6 and TNF- alpha in the cerebrospinal fluid of patients with acute viral encephalitis without epileptic seizures. Epileptic seizures in acute phase of toxic encephalitis are related to elevated levels of IL-1, IL-6 and TNF- alpha in CSF.
【學位授予單位】：山西醫(yī)科大學
【學位級別】：碩士
【學位授予年份】：2017
【分類號】：R512.3;R742.1

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