本文選題：慢性乙肝 + 中性粒細(xì)胞��； 參考：《安徽理工大學(xué)》2014年碩士論文

【摘要】：目的：探討慢性乙肝患者外周血CXCL8含量及中性粒細(xì)胞CXCL8、CXCR1、 CXCR2的表達(dá)水平。觀察HBV侵染中性粒細(xì)胞后對CXCL8及其受體CXCR1、 CXCR2表達(dá)的影響。方法：篩選慢性乙肝患者42例,其中HBeAg陽性、陰性患者分別為17和25例,HBV-DNA陽性和陰性患者分別為13例和29例,以ELISA法檢測患者血清CXCL8水平,Trizol提取PMNs總RNA,并逆轉(zhuǎn)錄為cDNA, qPCR法檢測慢性乙肝PMNs內(nèi)CXCL8、CXCR1、CXCR2mRNA表達(dá)量,以lgcDNA/lgGAPDH代表其最終mRNA水平。進(jìn)一步觀察血清CXCL8含量及PMNs內(nèi)CXCL8、CXCR1、CXCR2mRNA表達(dá)水平與血清ALT水平的相互關(guān)系。采用SABC細(xì)胞免疫化學(xué)染色法觀察患者外周血PMNs內(nèi)CXCL8、CXCR1、CXCR2的表達(dá),對比觀察HBV侵染PMNs后對CXCL8、CXCR1、CXCR2表達(dá)的影響。 結(jié)果：慢性乙肝患者血清CXCL8含量為(432.43±216.33) pg/mL。其中, HBeAg(+)與HBeAg (-)患者CXCL8含量分別為(612.15±165.56) pg/mL和(310.21±152.42) pg/mL,差異有統(tǒng)計(jì)學(xué)意義(P0.01). HBV-DNA (+)與HBV-DNA (-)患者CXCL8含量分別為(593.74±174.03) pg/mL和(360.11±195.05) pg/mL,差異亦有統(tǒng)計(jì)學(xué)意義(P0.01)�；颊咄庵苎狿MNs內(nèi)CXCL8、CXCR1的mRNA水平分別為1.12±0.21、0.86±0.37,較正常對照組明顯升高(P0.01)。其中HBeAg(+)患者CXCL8、CXCR1的mRNA水平分別為1.54±0.30、1.01±0.34,與HBeAg(-)組對照組相比,差異有顯著性(P0.05);HBeAg(+)患者CXCR2的mRNA水平為1.21±0.15,與HBeAg(-)患者1.13±0.32相比,差異無統(tǒng)計(jì)學(xué)意義(P0.05)。HBV-DNA(+)組與HBV-DNA(-)組相比,CXCL8、CXCR1的mRNA水平分別為1.22±0.26、1.05±0.21與1.08±0.18、0.79±0.42,差異有顯著性(P0.05)；CXCR2的mRNA水平分別為1.13±0.25和1.17±0.29,差異無統(tǒng)計(jì)學(xué)意義(P0.05)。血清CXCL8水平與ALT異常升高呈正相關(guān)0=0.859, P0.01), PMNs內(nèi)CXCL8. CXCR1的mRNA與ALT異常升高亦有一定相關(guān)性(r=0.688,P0.01；r=0.585,P0.01),而CXCR2表達(dá)與ALT異常升高無明顯相關(guān)性(r=0.088,P0.05)。SABC免疫細(xì)胞化學(xué)染色結(jié)果顯示,CXCL8主要位于PMNs胞漿中,CXCR1、 CXCR2多見于胞漿和胞膜上。其中HBeAg(+)者CXCL8、CXCR1免疫著色較深,而CXCR2免疫著色較淺；PMNs內(nèi)HBVDNA(+)者CXCL8、CXCR1免疫著色亦較深,而CXCR2免疫著色亦較淺。與對照組相比,CXCL8、CXCR1的表達(dá)水平差異均有顯著性(P0.05),而CXCR2的表達(dá)無統(tǒng)計(jì)學(xué)意義(P0.05)。 結(jié)論：HBV除對肝細(xì)胞具有較高親嗜性,還能侵染中性粒細(xì)胞,形成肝外潛伏或隱匿感染,此可能是導(dǎo)致慢性乙肝病情遷延反復(fù)、久治難愈的重要病因。慢性乙肝患者外周血CXCL8含量增高,PMNs內(nèi)CXCL8、CXCR1、CXCR2的mRNA水平增高,其中,血清CXCL8水平及CXCL8、CXCR1的mRNA與血清ALT含量呈正相關(guān)。HBV侵染中性粒細(xì)胞后可干擾宿主細(xì)胞正常代謝,使CXCL8分泌增加,細(xì)胞膜上CXCR1、CXCR2表達(dá)增強(qiáng)。CXCL8主要位于PMNs胞漿中,CXCR1、 CXCR2多見于胞漿和胞膜上,其著色程度與患者HBeAg表達(dá)、HBV DNA載量密切相關(guān)。HBV侵染中性粒細(xì)胞后可使胞內(nèi)分泌釋放CXCL8進(jìn)一步增加,胞膜CXCR1表達(dá)進(jìn)一步增強(qiáng)。高表達(dá)CXCR1的中性粒細(xì)胞又與CXCL8相互作用,趨化吸引更多中性粒細(xì)胞至病灶,參與局部炎性損傷和組織修復(fù)。
[Abstract]:Objective: To investigate the CXCL8 content of peripheral blood and the expression level of CXCL8, CXCR1 and CXCR2 of neutrophils in patients with chronic hepatitis B. The effect of HBV on the expression of CXCL8 and its receptor CXCR1 and CXCR2 after HBV infection was observed. Methods: 42 cases of chronic hepatitis B were screened, of which 17 and 25 negative patients were positive and negative, and HBV-DNA positive and negative. The patients were 13 cases and 29 cases respectively. The serum CXCL8 level was detected by ELISA. The total RNA of PMNs was extracted by Trizol and cDNA. The qPCR method was used to detect the CXCL8, CXCR1, CXCR2mRNA expression in PMNs. The relationship between serum ALT level and the expression of CXCL8, CXCR1 and CXCR2 in peripheral blood PMNs were observed by SABC cell immunochemistry staining, and the effects of HBV infection on CXCL8, CXCR1, and CXCR2 expression were observed.
Results: the serum CXCL8 content of patients with chronic hepatitis B was (432.43 + 216.33) pg/mL., and the CXCL8 content of HBeAg (+) and HBeAg (-) patients was (612.15 + 165.56) pg/mL and (310.21 + 152.42) pg/mL respectively, the difference was statistically significant (P0.01). The CXCL8 content of HBV-DNA (+) and HBV-DNA (-) patients was (593.74 + 174.03) pg/mL and (360.11 + 195.05) respectively. G/mL, the difference was also statistically significant (P0.01). The level of CXCL8 and CXCR1 in the peripheral blood of the patients was 1.12 + 0.21,0.86 + 0.37, respectively, which was significantly higher than that in the normal control group (P0.01). The HBeAg (+) patients' CXCL8, CXCR1 mRNA level was 1.54 + 0.34, respectively, and the difference was significant compared with the control group (+). The mRNA level of CXCR2 was 1.21 + 0.15, compared with 1.13 + 0.32 of HBeAg (-) patients, the difference was not statistically significant (P0.05) in.HBV-DNA (+) group, the mRNA level of CXCL8, CXCR1 was 1.22 + 0.26,1.05 + 0.21 and 1.08 + 0.18,0.79 + 0.42 respectively, and the difference was significant (P0.05), and the levels were 1.13 + 0.25 and 1.17 + 0.29, respectively. There was no statistical significance (P0.05). The level of serum CXCL8 was positively correlated with the abnormal increase of ALT, 0=0.859, P0.01), and there was a correlation between mRNA and ALT abnormal increase of CXCL8. CXCR1 in PMNs. CL8 is mainly located in the cytoplasm of PMNs, and CXCR1 and CXCR2 are more common in the cytoplasm and cell membrane. Among them, HBeAg (+) is CXCL8, CXCR1 immunologic coloring is deeper, CXCR2 immune coloring is shallow, PMNs HBVDNA (+) CXCL8, CXCR1 immune coloring is also deep, and the immune coloring is also shallow. However, the expression of CXCR2 was not statistically significant (P0.05).
Conclusion: HBV has high affinity to liver cells, but also can infect neutrophils and form extrahepatic latent or concealed infection. This may be an important cause of chronic hepatitis B relapse and prolonged treatment. The content of CXCL8 in peripheral blood of chronic hepatitis B patients is higher, and the level of mRNA in CXCL8, CXCR1 and CXCR2 in PMNs is higher, in which the serum CXCL8 water is increased. CXCL8, CXCR1 mRNA and serum ALT content positively correlated with.HBV infection of neutrophils, which can interfere with normal metabolism of host cells and increase the secretion of CXCL8. The expression of CXCR1 and CXCR2 on the cell membrane is mainly in the PMNs cytoplasm, CXCR1, CXCR2 is mostly seen in the cytoplasm and the cytoplasm, and the degree of coloring is closely related to the patient's expression. After related.HBV infection of neutrophils, the endocrine release of CXCL8 can be further increased and the expression of CXCR1 in the cell membrane is further enhanced. The neutrophils with high expression of CXCR1 interact with CXCL8, and chemotaxis attract more neutrophils to the focus, and participate in local inflammatory injury and tissue repair.
【學(xué)位授予單位】：安徽理工大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2014
【分類號】：R512.62

【參考文獻(xiàn)】

相關(guān)期刊論文 前1條

1 韓忠燕;王健;;慢性乙型肝炎患者中性粒細(xì)胞中乙型肝炎病毒的檢測和分析[J];細(xì)胞與分子免疫學(xué)雜志;2014年03期

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本文編號：1985270