本文選題：廣州管圓線蟲 + 程海��； 參考：《大理學院》2014年碩士論文

【摘要】：【目的】 云南瀘沽湖、程海流域進行廣州管圓線蟲流行病學調查，初步掌握云南瀘沽湖、程海流域廣州管圓線蟲中間宿主和終宿主的分布及感染情況，檢測該流域內人群廣州管圓線蟲感染情況；結合地理信息系統(tǒng)建立云南瀘沽湖、程海流域廣州管圓線蟲流行病學調查數(shù)據(jù)庫，分析影響廣州管圓線蟲流行及分布的因素。 【方法】 廣州管圓線蟲中間宿主螺螄和蛞蝓采用胃蛋白酶消化法檢查其感染狀況；終宿主鼠采用剖檢法和免疫酶聯(lián)法檢查其感染狀況；GPS定位儀實地定位中間宿主和終宿主的調查點的經(jīng)緯度和海拔；云南瀘沽湖、程海流域人群作流行病學問卷調查，免疫酶聯(lián)法檢測被抽取人群血清的廣州管圓線蟲特異性Ig抗體；用Spss19.0對云南瀘沽湖、程海流域廣州管圓線蟲流行病學調查數(shù)據(jù)進行統(tǒng)計分析；結合地理信息系統(tǒng)建立云南瀘沽湖、程海流域廣州管圓線蟲流行病學調查數(shù)據(jù)庫；基于地理信息系統(tǒng)分析影響廣州管圓線蟲流行及分布的因素。 【結果】 1.捕獲廣州管圓線蟲中間宿主4種螺螄和1種蛞蝓，共3615只，經(jīng)檢測均未發(fā)現(xiàn)廣州管圓線蟲。瀘沽湖流域采集到3種螺螄和1種蛞蝓，分別是：中華圓田螺、銅銹環(huán)棱螺、琵琶蘿卜螺和復套蛞蝓；程海流域采集到4種螺螄和1種蛞蝓，分別是：中華圓田螺、銅銹環(huán)棱螺、琵琶蘿卜螺、釘螺和復套蛞蝓。 2.捕獲廣州管圓線蟲終宿主3種鼠，共234只，剖檢法均未發(fā)現(xiàn)廣州管圓線蟲；79份鼠血清酶聯(lián)免疫法檢測廣州管圓線蟲特異性IgG抗體,結果均為陰性。 3.采集云南瀘沽湖、程海流域居民血清548份，免疫酶聯(lián)法檢測廣州管圓線蟲特異性IgG抗體，共26份陽性血清，陽性率為4.74%。少數(shù)民族人群血清陽性率較高;有野外喝生水習慣的居民血清陽性率較高。 4.結合地理信息系統(tǒng)建立了云南瀘沽湖、程海流域廣州管圓線蟲流行病學調查快速查詢數(shù)據(jù)庫，并將調查數(shù)據(jù)進行了可視化描述。 5.云南瀘沽湖、程海流域距水域不同距離緩沖區(qū)內居民血清廣州管圓線蟲特異性IgG抗體陽性率差異無統(tǒng)計學意義；居民血清中廣州管圓線蟲特異性IgG抗體OD值空間分布不具有顯著的空間自相關性；在寧蒗縣與程海縣交界處存在廣州管圓線蟲特異性IgG抗體OD值高值的聚集區(qū)域，為該地區(qū)廣州管圓線蟲的防治提供了依據(jù)。 6.基于溫度分析結果顯示瀘沽湖、程海流域所在的寧蒗縣、永勝縣不適合廣州管圓線蟲的生長發(fā)育。 【結論】 1.云南瀘沽湖、程海流域均有廣州管圓線蟲傳播宿主的存在，但沒有發(fā)現(xiàn)廣州管圓線蟲蟲體，該區(qū)域的二月平均溫度較低，不適合廣州管圓線蟲的生長發(fā)育，云南瀘沽湖、程海流域暫不能視為廣州管圓線蟲自然疫源地。 2.云南瀘沽湖、程海流域居民血清標本進行廣州管圓線蟲特異性IgG抗體陽檢測發(fā)現(xiàn)26例陽性標本。其中，根據(jù)調查問卷統(tǒng)計顯示，少數(shù)民族居民血清陽性率高于漢族居民血清陽性率，，有野外喝生水習慣的居民血清陽性率高于沒有在野外喝生水習慣的居民。 3.利用地理信息系統(tǒng)對本次云南瀘沽湖、程海流域廣州管圓線蟲流行病學調查數(shù)據(jù)進行了可視化描述并將數(shù)據(jù)結合圖像建立了瀘沽湖、程海流域廣州管圓線蟲流行病學信息的快速查詢系統(tǒng)。 4.本次檢測云南瀘沽湖、程海流域居民血清廣州管圓線蟲特異性IgG抗體，發(fā)現(xiàn)其學清的OD值在整體上沒有空間自相關性，但在寧蒗縣與永勝縣的東南交界處附近可能存在血清OD值高值的聚集區(qū)域，該地區(qū)廣州管圓線蟲的防治工作不能松懈。
[Abstract]:[Objective]
Yunnan Lugu Lake and Cheng Hai Basin carried out an epidemiological survey of Guangzhou tube nematode, preliminarily grasps the distribution and infection of the Yunnan Lugu Lake, the middle host and the final host of the Guangzhou tube nematode in the Cheng Hai Valley, and detected the infection of the Guangzhou tube nematode in the river basin, and established the Yunnan Lugu Lake and the Guangzhou of the Cheng Hai Basin in combination with the geographic information system. The epidemiology and distribution of Guangzhou's roundworm were analyzed by the database of epidemiology.
[method]
The infection status of the snail and slug in the middle host of Guangzhou nematode was examined by pepsin digestion; the infection status of the final host mice was examined by caesarean section and immunoenzyme linked assay; the latitude and latitude of the investigation points of the intermediate host and the final host by GPS locator on the spot; the population of the Lugu Lake in Yunnan and the area of the Cheng Hai River Basin for epidemiology. A questionnaire survey was conducted to detect the specific Ig antibody of Guangzhou tube nematode in the serum of the extracted population, and the epidemiological survey data of Guangzhou tube nematode in Yunnan Lugu Lake and Cheng Hai Basin were statistically analyzed with Spss19.0. The epidemiological investigation of Guangzhou tube nematode in the Yunnan Lugu Lake and the Cheng Hai River Basin were established by combining the geographic information system. The geographic information system (GIS) was used to analyze the factors affecting the prevalence and distribution of Guangzhou C. elegans.
[results]
1. a total of 4 species of snail and 1 slugs in the middle host of Guangzhou nematode were captured, and 3615 were found. There were 3 species of snails and 1 slugs in the Lugu Lake basin. They were Chinese snail, rounded spiral snail, lute, and multiple slugs, and 4 species of snail and 1 slugs were collected in the Cheng Hai basin, respectively: Hua Yuan snail, rust rust, snails, lute, snails, snails and compound slugs.
2. a total of 3 mice were captured in the final host of Guangzhou tube nematode, and there were 234 rats. No Guangzhou tube nematode was found in the caesarean section. 79 mice of serum enzyme linked immunosorbent assay (ELISA) were used to detect the specific IgG antibody of the Guangzhou tube nematode. The results were all negative.
3. in Yunnan Lugu Lake, 548 sera were collected from the residents of the Cheng Hai River Basin, and the immunoenzyme linked immunosorbent assay was used to detect the specific IgG antibody of the Guangzhou tube nematode, with a total of 26 positive sera. The positive rate of the positive rate was higher in the 4.74%. minority group, and the positive rate of the residents with the habit of drinking water in the field was higher.
4. combined with geographic information system, Yunnan Lugu Lake, Guangzhou River Valley nematode epidemiological investigation database was established, and the survey data were visualized.
5. Yunnan Lugu Lake, there was no significant difference in the positive rate of sera specific IgG antibody of Guangzhou tube nematode, and there was no significant spatial autocorrelation in the spatial distribution of the specific IgG antibody of Guangzhou tube nematode in the residents' sera, and there was Guangzhou at the junction of the county of Ning Lang County and Cheng Hai county. The aggregation area of the high OD value of the specific IgG antibody of C.elegans provides a basis for the prevention and treatment of the Guangzhou roundworm.
6. based on the results of temperature analysis, it is shown that the Yongsheng Lugu Lake is not suitable for the growth and development of the Guangzhou roundworm.
[Conclusion]
1. Yunnan Lugu Lake, Cheng Hai Basin all the host of Guangzhou tube nematode transmission, but not the Guangzhou tube round line worm body, the area of the average temperature in February is low, it is not suitable for the growth and development of Guangzhou tube nematode, Yunnan Lugu Lake, the Cheng Hai Basin can not be considered as the natural foci of the Guangzhou tube nematode.
2. Yunnan Lugu Lake, the serum samples of the residents in the Cheng Hai River Basin were tested for 26 positive specimens of the specific IgG antibody positive of Guangzhou tube nematode. Among them, the positive rate of the serum positive rate of the ethnic minority residents was higher than that of the Han population, and the positive rate of the people who had the habit of drinking water in the field was higher than that in the field. A inhabitant of the habit of drinking water.
3. the geographical information system was used to visualize the epidemiological survey data of Guangzhou tube nematode in Yunnan Lugu Lake and Cheng Hai River Basin, and to set up a rapid inquiry system for the epidemiological information of the Guangzhou tube nematode in the Lugu Lake and the Cheng Hai Basin by combining the data with the data.
4. in Yunnan Lugu Lake, the residents of the Cheng Hai River Basin were found to have a serum specific IgG antibody of Guangzhou tube nematode. It was found that there was no spatial autocorrelation on the whole, but there might be a high concentration of serum o value in the vicinity of the southeastern border of Yongsheng county. The prevention and treatment of Guangzhou tube nematode in this area could not be loose. Lax.
【學位授予單位】：大理學院
【學位級別】：碩士
【學位授予年份】：2014
【分類號】：R532.1

【參考文獻】

相關期刊論文 前10條

1 鄧卓暉;張啟明;林榮幸;黃少玉;張賢昌;呂山;劉和香;胡鈴;裴福全;崔惠兒;王金龍;;廣東省廣州管圓線蟲病自然疫源地調查[J];華南預防醫(yī)學;2008年04期

2 楊坤;王顯紅;呂山;張玲;賈鐵武;李蘭花;鄧瑤;周曉農;;氣候變暖對中國幾種重要媒介傳播疾病的影響[J];國際醫(yī)學寄生蟲病雜志;2006年04期

3 張鴻滿，陶甲芬，胡文慶;南寧市廣州管圓線蟲中間宿主感染情況的調查研究[J];廣西預防醫(yī)學;1996年02期

4 陳潔芳;邱偉文;王苑華;;生食蛞蝓致廣州管圓線蟲感染引起嗜酸細胞性腦脊膜神經(jīng)根炎1例[J];疾病監(jiān)測;2007年12期

5 郭玉紅;郎南軍;楊文燦;江期川;張立新;;云南高原山地林分郁閉度與釘螺密度關系的研究[J];濕地科學與管理;2011年01期

6 何銘謙;章家恩;羅明珠;宋春秀;趙本良;葉延瓊;嚴家躍;;基于GIS的廣東地區(qū)福壽螺的潛在時空分布及對稻田的危害風險[J];華南農業(yè)大學學報;2012年03期

7 何漢江;呂志躍;李振宇;余新炳;吳忠道;;廣州管圓線蟲對不同宿主感染性的比較研究[J];熱帶醫(yī)學雜志;2009年02期

8 傅先源,王洪全;溫度對福壽螺生長發(fā)育的影響[J];水產學報;1999年01期

9 陳紹榮;呂山;汪麗波;方文;陳鳳;李科榮;杜遵偉;張儀;姜進勇;李遠林;周曉農;張再興;;云南省大理州首次廣州管圓線蟲病疫情調查與處置[J];寄生蟲病與感染性疾病;2008年03期

10 惠新民,張世杰,曹彬,嚴渭民;野蛞蝓發(fā)生規(guī)律及綜合防治技術[J];陜西農業(yè)科學;2003年02期

相關博士學位論文 前1條

1 楊坤;血吸蟲病景觀格局與貝葉斯復合模型的構建[D];中國疾病預防控制中心;2008年

本文編號：1954446