本文選題：登革熱 + 登革病毒　； 參考：《中國病原生物學(xué)雜志》2017年05期

【摘要】：目的評價我國研制的酶聯(lián)免疫試劑檢測登革熱病毒的效能,推進國產(chǎn)登革熱檢測劑的研發(fā)。方法采用我國研制的酶聯(lián)免疫試劑對已知登革熱NS1陽性血清和陰性血清進行檢測,對已知陽性血清中檢測陰性的血清和已知陰性血清中檢測陽性血清進行PCR擴增,以排除假陰性或假陽性。根據(jù)檢測結(jié)果,計算陽性符合率(真陽性率)、陰性符合率(真陰性率),進行配對資料的χ2檢驗,對試劑盒的靈敏度和特異性等進行綜合評估。結(jié)果國產(chǎn)酶聯(lián)免疫試劑檢測已知登革熱患者血清108份均陽性,陽性符合率(真陽性率)為100%,假陰性率為0。檢測139份已知陰性血清,其中127份陰性,12份陽性(核酸檢測為陰性),陰性符合率(真陰性率)為91.37%,假陽性率為8.63%。檢測15份干擾標(biāo)本均陰性,干擾標(biāo)本符合率為100%。國產(chǎn)酶聯(lián)免疫試劑與美國NS1試劑盒檢測的總符合率為95.14%,兩種方法的檢測結(jié)果差異有統(tǒng)計學(xué)意義(χ~2=4.6,P0.05)。結(jié)論國產(chǎn)酶聯(lián)免疫試劑檢測登革熱的靈敏度高,但特異性較美國NS1試劑盒差。
[Abstract]:Objective to evaluate the efficacy of enzyme linked immunosorbent (Elisa) developed in China for the detection of dengue virus and to promote the development of domestic dengue detection agent. Methods NS1 positive serum and negative serum of dengue fever were detected by enzyme-linked immunosorbent assay (Elisa) developed in China, and PCR amplification was carried out in the serum that was negative and negative in known positive serum. To exclude false negative or false positive. According to the test results, the positive coincidence rate (true positive rate) and negative coincidence rate (true negative rate) were calculated. The 蠂 2 test of paired data was carried out, and the sensitivity and specificity of the kit were comprehensively evaluated. Results 108 serum samples of known dengue fever patients were detected by domestic enzyme linked immunosorbent assay (Elisa). The positive coincidence rate (true positive rate) was 100 and false negative rate was 0. Of 139 known negative sera, 127 were negative and 12 were positive (nucleic acid test was negative, negative coincidence rate (true negative rate) was 91.37, false positive rate was 8.63. All 15 samples were negative, and the coincidence rate was 100%. The total coincidence rate between the domestic Elisa kit and the NS1 kit was 95.14, and the difference between the two methods was statistically significant (蠂 ~ (2 +) 4.6U P0.05). Conclusion Enzyme-linked immunosorbent assay is more sensitive than NS1 kit in detecting dengue fever.
【作者單位】： 曲靖市第一人民醫(yī)院生物實驗室;昆明市中醫(yī)醫(yī)院康復(fù)科;大理大學(xué)病原生物研究所;云南省寄生蟲病防治所云南省瘧疾研究中心;
【基金】：云南省惠民科技計劃項目(No.2016RA010) 上海市2015年度“科技創(chuàng)新行動計劃”國內(nèi)合作項目(No.15495800400)
【分類號】：R512.8
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本文編號：1929166