本文選題：瘧原蟲 + 免疫膠體金法�。� 參考：《中國醫(yī)藥導(dǎo)報(bào)》2016年34期

【摘要】：目的評價(jià)一種新型惡性瘧和間日瘧聯(lián)合快速檢測膠體金試劑盒的檢測性能。方法對2001年1月~2016年4月解放軍第三0二醫(yī)院及協(xié)助醫(yī)院留取的瘧原蟲感染患者及其他發(fā)熱患者全血樣本345例,分別采用瘧原蟲免疫膠體金法與顯微鏡檢法進(jìn)行瘧原蟲檢測,以顯微鏡檢法結(jié)果為金標(biāo)準(zhǔn)評價(jià)免疫膠體金快速檢測試劑的靈敏度、特異度和一致性。結(jié)果發(fā)熱患者中血涂片鏡檢法檢出瘧原蟲感染204例,快速檢測膠體金試劑盒檢出瘧原蟲感染207例。其中惡性瘧原蟲免疫膠體金法檢出111例,顯微鏡檢法檢出112例,靈敏度為99.11%,特異度為100.00%,兩種方法一致性檢驗(yàn)Kappa值為0.993,兩種檢測結(jié)果具有很好的一致性。間日瘧原蟲免疫膠體金法檢測出96例,顯微鏡檢法檢出92例,靈敏度為100.00%,特異度為98.42%,兩種方法的一致性檢驗(yàn)Kappa值0.971,兩種檢測結(jié)果具有很好的一致性。結(jié)論新型快速檢測試劑盒瘧原蟲抗體免疫膠體金法與顯微鏡檢法結(jié)果具有較好的一致性,且具有操作簡便、快速、無需專業(yè)儀器和專門技術(shù)人員等優(yōu)點(diǎn),適合于臨床實(shí)驗(yàn)室瘧疾的輔助診斷和篩查。
[Abstract]:Objective to evaluate the detection performance of a new type of colloidal gold kit for rapid detection of falciparum malaria (falciparum malaria) and vivax malaria (vivax malaria). Methods from January 2001 to April 2016, 345 whole blood samples of patients with malaria parasite infection and other febrile patients were collected from the 30th and 2nd Hospital of PLA and the assisting Hospital. The Plasmodium malaria parasites were detected by the method of immuno-colloidal gold and microscope, respectively. The sensitivity, specificity and consistency of immuno-colloidal gold rapid detection reagent were evaluated by using the results of microscope as gold standard. Results 204 cases of Plasmodium infection were detected by blood smear microscopy and 207 cases were detected by rapid detection of colloidal gold kit. Among them, 111 cases of Plasmodium falciparum were detected by immuno-colloidal gold method, 112 cases were detected by microscope, the sensitivity was 99.11 and the specificity was 100.000.The Kappa value of the two methods was 0.993.The results of the two methods were in good agreement. 96 cases of Plasmodium vivax were detected by immuno-colloidal gold method, 92 cases were detected by microscope, the sensitivity was 100.00and the specificity was 98.42. The Kappa value of the two methods was 0.971. Conclusion the results of the new rapid detection kit for Plasmodium falciparum antibody are in good agreement with the results of microscope, and have the advantages of simple operation, fast operation and no need of professional instruments and technicians. It is suitable for assistant diagnosis and screening of malaria in clinical laboratory.
【作者單位】： 全軍感染病臨床實(shí)驗(yàn)診斷中心解放軍第三0二醫(yī)院臨床檢驗(yàn)醫(yī)學(xué)中心;
【基金】：國家重大新藥創(chuàng)制課題(2013ZX09J13106-06A) 解放軍第三0二醫(yī)院院長創(chuàng)新課題
【分類號】：R531.3
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本文編號：1892745