本文選題：維拉帕米 + 丙型肝炎病毒��； 參考：《第二軍醫(yī)大學學報》2017年05期

【摘要】：目的探討抗高血壓藥維拉帕米是否可通過下調(diào)宿主硫氧還蛋白互作蛋白(TXNIP)的表達而抑制丙型肝炎病毒(HCV)的感染。方法用不同濃度梯度的維拉帕米處理人肝癌細胞系Huh7.5.1,用qPCR和蛋白質(zhì)印跡法檢測TXNIP的表達。用維拉帕米處理Huh7.5.1細胞,同時加入細胞培養(yǎng)產(chǎn)生的HCV(HCVcc),48 h后檢測HCVcc感染情況。用TXNIP siRNA轉(zhuǎn)染Huh7.5.1細胞,檢測下調(diào)TXNIP表達后,維拉帕米對HCVcc感染的影響;用TXNIP啟動子調(diào)控的增強型綠色熒光蛋白(EGFP)報告基因表達質(zhì)粒(pTXNIP-EGFP)轉(zhuǎn)染Huh7.5.1細胞,分析維拉帕米對TXNIP啟動子轉(zhuǎn)錄活性的影響。結(jié)果與對照孔相比,維拉帕米(100、200、400μmol/L)可下調(diào)Huh7.5.1細胞中TXNIP的表達,并呈現(xiàn)濃度依賴性(P0.05);并可抑制HCVcc對Huh7.5.1細胞的感染,且濃度越高抑制作用越明顯(P0.05)。進一步研究結(jié)果顯示,與對照組相比,維拉帕米能夠抑制pTXNIP-EGFP轉(zhuǎn)染細胞中EGFP的表達水平(P0.05)。結(jié)論維拉帕米可下調(diào)TXNIP的表達從而抑制HCV感染,這可能是通過抑制TXNIP啟動子的轉(zhuǎn)錄活性來實現(xiàn)的。
[Abstract]:Objective to investigate whether verapamil can inhibit hepatitis C virus (HCV) infection by down-regulating the expression of host thioredoxin interaction protein (TXNIPP). Methods Human hepatoma cell line Huh 7.5.1 was treated with verapamil at different concentrations and the expression of TXNIP was detected by qPCR and Western blotting. Huh7.5.1 cells were treated with verapamil and the HCVcc infection was detected after 48 h. The effect of verapamil on HCVcc infection was detected after down-regulation of TXNIP expression in Huh7.5.1 cells transfected with TXNIP siRNA, and pTXNIP-EGFP) was transfected into Huh7.5.1 cells by TXNIP promoter regulated enhanced green fluorescent protein (EGFP) reporter gene expression plasmid pTXNIP-EGFP. To analyze the effect of verapamil on the transcriptional activity of TXNIP promoter. Results verapamil 100200400 渭 mol / L could down-regulate the expression of TXNIP in Huh7.5.1 cells in a concentration-dependent manner, and inhibit the infection of Huh7.5.1 cells by HCVcc. The higher the concentration of verapamil was, the more obvious the inhibitory effect was on Huh7.5.1 cells. Further studies showed that verapamil could inhibit the expression of EGFP in pTXNIP-EGFP transfected cells compared with the control group (P 0.05). Conclusion verapamil can down-regulate the expression of TXNIP and inhibit HCV infection, which may be achieved by inhibiting the transcriptional activity of TXNIP promoter.
【作者單位】： 第二軍醫(yī)大學熱帶醫(yī)學與公共衛(wèi)生學系微生物學教研室上海市醫(yī)學生物防護重點實驗室;第二軍醫(yī)大學海軍醫(yī)學系學員5隊;第二軍醫(yī)大學學員旅10隊;
【基金】：國家自然科學基金(31570170) 上海市公共衛(wèi)生三年行動計劃重點學科建設項目(15GWZK0103)~~
【分類號】：R512.63

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