本文選題：骨橋蛋白 + 抗體�。� 參考：《石河子大學(xué)》2013年碩士論文

【摘要】：目的：建立沙鼠肝泡狀棘球蚴動物模型，外源性給予抗OPN抗體，根據(jù)在不同生長階段，沙鼠肝泡球蚴及轉(zhuǎn)移組織中IL-2、IL-5、IL-10及TNF-α因子的表達水平，來探討這四種因子在沙鼠肝泡狀棘球蚴侵襲性生長和轉(zhuǎn)移中的作用，觀察抗OPN抗體對泡狀棘球蚴生長發(fā)育的影響。 方法：選擇4-6周齡健康長爪沙鼠120只，雌性，體重（30±5）g。隨機分為三組，采用開腹直視下肝臟穿刺接種多房棘球蚴原頭節(jié)混懸液的方法(0.15ml/只)，建立肝泡狀棘球蚴動物模型。一組單純接種泡狀棘球蚴組（模型組），二組在接種泡狀棘球蚴同時予以抗OPN抗體干預(yù)（實驗組），三組在接種泡狀棘球蚴同時予以兔血清干預(yù)（對照組）。分別將三組接種泡狀棘球蚴的沙鼠隨機分成6小組，每小組10只，分別為感染140天、180天、220天、260天組。于相應(yīng)時間處死各組沙鼠，觀察泡狀棘球蚴的生長、轉(zhuǎn)移等情況；免疫組化法測定肝泡狀棘球蚴及轉(zhuǎn)移組織標(biāo)本中的IL-2，IL-5，IL-10及TNF-α因子的表達。 結(jié)果： 1.140天、180天、220天組時實驗組與對照組、實驗組與模型組囊濕重有顯著性差異（F值分別為8.647，36.96，9.12，p值均小于0.05），，而對照組與模型之間比較無差異（P㧐0.05）。 2.觀察泡狀棘球蚴轉(zhuǎn)移現(xiàn)象，發(fā)現(xiàn)肺轉(zhuǎn)移灶較少見，而胸廓淋巴結(jié)轉(zhuǎn)移率較高，未見腦轉(zhuǎn)移灶。統(tǒng)計結(jié)果顯示：140天、180天、組時實驗組與對照組、實驗組與模型組胸廓淋巴結(jié)轉(zhuǎn)移率有顯著性差異（P㩳0.05），220天組時實驗組與對照組，實驗組與模型組胸廓淋巴結(jié)轉(zhuǎn)移率無顯著性差異（P㧐0.05）。 3. HE染色結(jié)果顯示:在140天、180天、220天時間段，實驗組與對照組、實驗組與模型組間E.m囊壁病理的改變無顯著性差異（P㧐0.05）。 4.免疫組化染色結(jié)果顯示：肝泡型棘球蚴組織中可見IL-2，IL-5， IL-10及TNF-α因子不同程度的表達，定位于細胞漿，主要分布在肝泡狀棘球蚴纖維囊壁的生發(fā)層，細胞漿中可見黃色、棕黃色或棕褐色等陽性顆粒；140天、180天組時IL-5，IL-10的表達在實驗組與對照組、實驗組與模型組比較有顯著性差異（P㩳0.05）；140天、180天組時時間段，實驗組與對照組、實驗組與模型組IL-2及TNF-α的陽性細胞率比較無顯著性差異（P㧐0.05），220天時時間段，實驗組與對照組、實驗組與模型組IL-2，IL-5，IL-10及TNF-α的陽性細胞率比較無顯著性差異。 結(jié)論：IL-2，IL-5，IL-10及TNF-α共同參與了宿主與肝泡狀棘球蚴相互間中晚期免疫進程。外源性給予抗OPN抗體可使肝泡狀棘球蚴組織中IL-5，IL-10的表達減低，這可能是抑制肝泡狀棘球蚴中晚期侵襲性生長和轉(zhuǎn)移的機制之一。
[Abstract]:Objective: to establish an animal model of hepatic alveolar echinococcosis in gerbils, and give exogenous anti OPN antibody. According to the expression levels of IL-2, IL-5, IL-10 and TNF- 偽 in liver alveolar hydatid and metastatic tissues of gerbils at different growth stages. To investigate the role of these four factors in the invasive growth and metastasis of hydatid alveolar hydatid in gerbils, and to observe the effect of anti OPN antibody on the growth and development of hydatid alveolar hydatid. Methods: 120 healthy gerbils aged 4-6 weeks, female, weight 30 鹵5g. Three groups were randomly divided into three groups. The hepatic alveolar hydatid model was established by liver puncture and inoculation of multilocularis echinococci with the suspension of the original head ganglion of Echinococcus multilocularis (Echinococcus multilocularis). One group was inoculated with alveolar echinococcus (model group), the other group was inoculated with OPN antibody (experimental group) and three groups were inoculated with rabbit serum (control group). Three groups of gerbils inoculated with hydatid alveolar echinococci were randomly divided into 6 groups with 10 rats in each group. The growth and metastasis of hydatid alveolar echinococci were observed and the expression of IL-2IL-5 IL-10 and TNF- 偽 in liver alveolar echinococci and metastatic tissues were detected by immunohistochemical method. Results: There was significant difference between the experimental group and the control group in the wet weight of the capsule between the experimental group and the control group at day 1.140 and day 180 and 220d, respectively. The F value of the experimental group and the model group was 8.647 / 36.96 / 9.12p < 0.05 respectively, but there was no significant difference between the control group and the model group (P < 0.05). 2. The phenomenon of alveolar echinococcosis metastasis was observed. The pulmonary metastasis was rare, but the thoracic lymph node metastasis rate was higher, and no brain metastasis was found. The statistical results showed that there were significant differences between the experimental group and the control group at 140 days to 180 days, and the rates of thoracic lymph node metastasis between the experimental group and the model group at 220 days. There was no significant difference between the experimental group and the model group in the rate of thoracic lymph node metastasis. 3. The results of HE staining showed that there was no significant difference between the experimental group and the control group and between the experimental group and the model group in the pathological changes of the E m cyst wall at the time of 140 days, 180 days and 220 days, and there was no significant difference between the experimental group and the control group. 4. Immunohistochemical staining showed that IL-2IL-5, IL-10 and TNF- 偽 were expressed in different degrees in the liver alveolar hydatid tissues, located in the cytoplasm, mainly distributed in the germinal layer of the fibrous wall of the hepatic alveolar echinococcus, and yellow in the cytoplasm. The expression of IL-5 IL-10 in the experimental group and the control group was significantly different from that in the experimental group and the model group at the time of 140 days and 180 days respectively, and the experimental group and the control group had a significant difference in the expression of IL-5 and IL-10 during the period of 140 days and 180 days, respectively, between the experimental group and the model group, and between the experimental group and the control group. There was no significant difference in the positive cell rates of IL-2 and TNF- 偽 between the experimental group and the model group. There was no significant difference between the experimental group and the control group, between the experimental group and the model group in the positive cell rate of IL-10 and TNF- 偽. Conclusion IL-10 and TNF- 偽 play an important role in the immune process between the host and the liver alveolar hydatid. Exogenous anti OPN antibody can reduce the expression of IL-5 and IL-10 in liver alveolar hydatid tissues, which may be one of the mechanisms of inhibiting the invasive growth and metastasis of hepatic alveolar echinococci.
【學(xué)位授予單位】：石河子大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2013
【分類號】：R512.62

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