本文選題：乙型肝炎病毒 + 乙肝病毒大蛋白��； 參考：《浙江大學(xué)》2014年碩士論文

【摘要】：1背景 世界上有20億人感染過HBV,目前全世界慢性HBV感染患者至少有3.8億,其中慢性HBV攜帶者75%分布在東南亞和次撒哈拉地區(qū),而我國就占了1.5億,其中有200萬人為慢性乙肝患者,每年因此病死亡的人已近50萬。估計全球每年有108-200萬人直接死于HBV持續(xù)感染。我國是乙肝的高流行地區(qū),乙肝病毒感染是極為重要的公共衛(wèi)生問題。而在學(xué)生升學(xué)體檢、公務(wù)員體檢、征兵體檢以及餐飲等行業(yè)中都對乙肝病毒的檢測有一定的規(guī)定。因此體檢中如何真實、快速的反映體檢人員的乙肝情況成為急待解決的問題,目前主要采取檢測HBV-DNA載量的方法來評估HBeAg陰性患者體內(nèi)的乙肝病毒復(fù)制情況,但此方法操作繁瑣、成本投入大、實驗要求高等原因,難以推廣應(yīng)用于大批量體檢標(biāo)本的檢測。 2目的 通過檢測公務(wù)員、學(xué)生、士兵等體檢人員中乙肝病毒大蛋白(HBV-LP),乙肝病毒DNA(HBV DNA)以及乙肝兩對半(HBV-M)的情況,探討乙肝病毒大蛋白用于公務(wù)員、學(xué)生、士兵、餐飲人員等不同體檢人員中的意義,為乙肝的公共體檢領(lǐng)域提供新的檢測方法。 3方法 乙肝病毒大蛋白檢測采用酶聯(lián)免疫法,試劑由北京熱景生物技術(shù)公司提供,cut-off值為0.105；HBV-M血清標(biāo)志物檢測采用酶聯(lián)免疫法,試劑盒購自上海科華生物有限公司；HBV-DNA定量檢測采用實時熒光定量RT-PCR,試劑盒購自廣州達(dá)安生物公司,以HBV DNA拷貝數(shù)≥1×103/ml作為HBV-DNA陽性標(biāo)準(zhǔn)；谷丙轉(zhuǎn)氨酶檢測采用化學(xué)法,試劑盒購自上海申能生物有限公司,以上均嚴(yán)格按試劑說明書進(jìn)行操作,有效期內(nèi)使用。采用SPSS12.0軟件進(jìn)行統(tǒng)計學(xué)分析.計量資料用X±S表示。HBV-LP與HBV-DNA陽性率的比較采用χ2檢驗,p0.05判定有統(tǒng)計學(xué)意義；HBV-LP S/OD值與HBV-DNA拷貝數(shù)之間的相關(guān)性采用線性相關(guān)分析。 4結(jié)果 4.1HBV-LP法與HBV-DNA法檢測709例中HBsAg陽性的體檢人員的檢測結(jié)果差異無統(tǒng)計學(xué)意義,χ2值和P值分別為1.47和0.23。 4.2不同乙肝兩對半模式中HBV-LP法與HBV-DNA法的檢測結(jié)果差異均無統(tǒng)計學(xué)意義,χ2值和P值分別為2.67,0.60,0.00和0.10,0.44,1.00。 4.3HBV-LP S/OD值與HBV-DNA的拷貝數(shù)呈正相關(guān)性(r=0.959,P0.05)。 5結(jié)論乙型肝炎病毒大蛋白(HBV-LP)在能夠有效、真實地反映HBV病毒復(fù)制情況,該方法可以在公務(wù)員、學(xué)生、士兵等體檢中進(jìn)行推廣
[Abstract]:1 background There are 2 billion people in the world who have been infected with HBV. At present, there are at least 380 million patients with chronic HBV infection in the world. Among them, 75% of the chronic HBV carriers are in Southeast Asia and Sub-Saharan region, and China accounts for 150 million, of which 2 million are chronic hepatitis B patients. Nearly 500000 people die each year from the disease. An estimated 108 to 2 million people worldwide die of persistent HBV infection every year. Hepatitis B virus infection is an extremely important public health problem in China. However, there are certain regulations on HBV detection in students' entrance examination, civil servant physical examination, conscription medical examination and catering industry. Therefore, it is urgent to solve the problem of how to truly and quickly reflect the hepatitis B situation of people in physical examination. At present, the method of detecting HBV-DNA load is mainly used to evaluate HBV replication in HBeAg negative patients, but the operation of this method is cumbersome. Because of the high cost and high demand, it is difficult to be applied to the detection of large numbers of physical examination specimens. 2 purpose In order to explore the application of hepatitis B virus large protein in civil servants, students, soldiers and so on, the hepatitis B virus large protein (HBV-LPU), hepatitis B virus DNA(HBV DNA (HBV DNA(HBV DNA) and hepatitis B virus (HBV-MN) were used in civil servants, students, soldiers, etc. The significance of different physical examination personnel, such as catering personnel, provides a new method for the detection of hepatitis B in the field of public health examination. 3 method The detection of hepatitis B virus large protein was performed by enzyme linked immunosorbent assay (Elisa), and the serum marker of HBV-M was determined by enzyme linked immunosorbent assay (Elisa), which was provided by Beijing Hejing Biotechnology Company with a cut-off value of 0.105 渭 m. The kit was purchased from Shanghai Kehua Biological Co., Ltd. The quantitative detection of HBV-DNA was carried out by real-time fluorescence quantitative RT-PCR.The kit was purchased from Guangzhou Da'an Biological Company, with the copy number of HBV DNA 鈮，

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