本文選題：氣相色譜-質(zhì)譜聯(lián)用 + 正交偏最小二乘法-判別分析�。� 參考：《色譜》2015年04期

【摘要】：探索了乙型肝炎患者和健康人血清代謝組的差異,尋找與疾病相關(guān)的潛在標(biāo)志物。收集乙肝患者30例、健康對照35例,以氣相色譜-質(zhì)譜聯(lián)用技術(shù)作為研究平臺(tái),應(yīng)用主成分分析、正交偏最小二乘法-判別分析進(jìn)行模式識(shí)別,然后通過變量重要性因子、非參數(shù)檢驗(yàn),結(jié)合數(shù)據(jù)庫檢索篩選鑒定有差異的代謝物。確認(rèn)10個(gè)代謝物存在顯著差異,其中檸檬酸、烏頭酸、谷氨酰胺、N,N-二甲基甘氨酸、丙二酸與乙型肝炎患者組的相關(guān)性較好,受試者工作特征曲線下面積為0.975,具有較好的特異度和敏感度。因此這5個(gè)代謝物能夠作為潛在的區(qū)分乙型肝炎患者和正常人的血清小分子標(biāo)志物,有助于進(jìn)一步了解病理機(jī)制,確定治療目標(biāo)。
[Abstract]:To explore the difference of serum metabolism between patients with hepatitis B and healthy people, and to search for potential markers related to disease. A total of 30 patients with hepatitis B and 35 healthy controls were collected. Using gas chromatography-mass spectrometry (GC-MS) as the research platform, principal component analysis (PCA), orthogonal partial least squares (orthogonal partial least squares) discriminant analysis were used for pattern recognition, and then variable importance factors were used. Non-parametric test and database search screening were used to identify different metabolites. Among the 10 metabolites, citric acid, aconitic acid, glutamine N-dimethylglycine and malonic acid had good correlation with hepatitis B patients. The area under the operating characteristic curve was 0.975, which had good specificity and sensitivity. Therefore, these five metabolites can be used as potential small molecular markers to distinguish hepatitis B patients from normal people, which is helpful to further understand the pathological mechanism and determine the treatment target.
【作者單位】： 上海交通大學(xué)藥學(xué)院;大連醫(yī)科大學(xué)腫瘤干細(xì)胞研究院;大連醫(yī)科大學(xué)附屬第一醫(yī)院;
【基金】：國家自然科學(xué)基金項(xiàng)目(21175092,21105064) 國家重大科學(xué)儀器設(shè)備開發(fā)專項(xiàng)(2011YQ150072,2011YQ15007204,2011YQ15007207,2011YQ15007210) 上海市自然科學(xué)基金項(xiàng)目(12ZR1413600)
【分類號】：R512.62;O657.63

【參考文獻(xiàn)】

相關(guān)期刊論文 前2條

1 Jing-Jing Ren;Ying Liu;Wen Ren;Yan Qiu;Bing Wang;Ping Chen;Kai-Jin Xu;Shi-Gui Yang;Jun Yao;Lan-Juan Li;;Role of general practitioners in prevention and treatment of hepatitis B in China[J];Hepatobiliary & Pancreatic Diseases International;2014年05期

2 余欣尉;吳謙;呂望;王彥;馬小瓊;陳U，

本文編號：1816712