本文選題：慢性丙型肝炎 + 腫瘤壞死因子-α��； 參考：《新疆醫(yī)科大學(xué)》2013年碩士論文

【摘要】：目的：研究慢性丙型肝炎患者血清TNF-α、IL-10水平及其與HCV RNA載量、ALT、AST水平的關(guān)系,并探討其導(dǎo)致肝臟損害的機制。方法：用酶聯(lián)免疫吸附法(ELISA)檢測112例慢性丙型肝炎患者、40例健康對照者血清中TNF-α、IL-10的水平。用PCR-熒光探針法檢測血清HCVRNA水平。使用全自動生化分析儀檢測ALT、AST含量。結(jié)果：慢性丙型肝炎患者組TNF-α、IL-10的水平均高于健康對照組(P=0.000,=0.000),在慢丙肝患者中,輕度組TNF-α,的水平均低于中度重度組,而輕度與中度組間差異無統(tǒng)計學(xué)意義(P=0.023,P=0.000,P=0.106),而IL-10水平在輕、中、重三組之間差異均無統(tǒng)計學(xué)意義(F=1.555,P=0.221)。隨著HCV RNA載量的升高,血清IL-10水平逐漸升高,各組之間差異有統(tǒng)計學(xué)意義(F=13.429,P=0.000)。而TNF-α在不同HCV RNA載量組之間均無顯著性差異(F=1.212,P=0.302)。TNF-α與ALT、AST呈正相關(guān),而與HCV RNA無相關(guān)性(P=O.000,P=0.000,P=0.105),IL-10與HCV RNA呈正關(guān),與ALT、AST無相關(guān)性(P=0.000,P=0.147,P=0.102), HCV RNA載量與ALT. AST水平無相關(guān)性(P=0.065,P=0.071)。結(jié)論：血清TNF-a水平隨著病情程度的加重升高,IL-10隨著HCV RNA載量升高而升高,TNF-α與IL-10是引起丙型肝炎肝臟損害及慢性化的重要因素,檢測TNF-α與IL-10水平可作為判斷慢性HCV感染情況的指標(biāo)。
[Abstract]:Objective: to study the relationship between serum TNF- 偽 -IL-10 level and HCV RNA load and alt level in patients with chronic hepatitis C, and to explore the mechanism of liver damage. Methods: the serum levels of TNF- 偽 and IL-10 in 112 patients with chronic hepatitis C and 40 healthy controls were detected by enzyme linked immunosorbent assay (Elisa). The serum HCVRNA level was detected by PCR- fluorescence probe method. The content of alt was measured by automatic biochemical analyzer. Results: the levels of TNF- 偽 IL-10 in patients with chronic hepatitis C were higher than those in healthy controls. In patients with chronic hepatitis C, the levels of TNF- 偽 in mild group were lower than those in moderate group, but there was no significant difference between mild group and moderate group. There was no significant difference among the three groups. With the increase of HCV RNA load, the serum IL-10 level increased gradually, and the difference between the groups was statistically significant. However, there was no significant difference in TNF- 偽 between different HCV RNA load groups. There was a positive correlation between TNF- 偽 and alt AST, but no correlation between TNF- 偽 and HCV RNA. There was no positive correlation between IL-10 and HCV RNA, and there was no correlation between TNF- 偽 and ALTAST, and there was no correlation between HCV RNA load and ALT. The level of AST was not correlated with 0.065 and 0.071. Conclusion: the level of serum TNF-a increases with the severity of the disease. IL-10 increases with the increase of HCV RNA load. TNF- 偽 and IL-10 are important factors causing liver damage and chronic hepatitis C. Detection of TNF- 偽 and IL-10 levels can be used as an index to judge chronic HCV infection.
【學(xué)位授予單位】：新疆醫(yī)科大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2013
【分類號】：R512.63

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本文編號：1811729