本文選題：諾如病毒 + 感染狀況　； 參考：《現(xiàn)代預(yù)防醫(yī)學(xué)》2017年22期

【摘要】：目的分析2015年鎮(zhèn)江地區(qū)諾如病毒的感染狀況及進(jìn)化重組現(xiàn)象。方法收集2015年鎮(zhèn)江地區(qū)哨點醫(yī)院腹瀉患者的糞便標(biāo)本及突發(fā)疫情的病例標(biāo)本,采用實時熒光定量PCR(q RT-PCR)方法進(jìn)行諾如病毒GI/GII核酸檢測,陽性樣本采用逆轉(zhuǎn)錄PCR(RT-PCR)方法擴增諾如病毒基因的Rd Rp區(qū)和Capsid區(qū),序列測定、基因分型,運用Mega軟件和RDP軟件進(jìn)行序列進(jìn)化和基因重組分析。結(jié)果 832份樣本中諾如病毒陽性率為5.05%(42/832),GII組陽性率為4.81%(40/832),占95.24%,GII組在10~20歲的中青年中陽性率最高,達(dá)25.00%(14/56)。2015年諾如病毒的檢出率以1月最高,11月、2月次之,以GII.17型所占比例最高為63.89%(23/36),新GII.17型變異株為最主要的流行株。主要的重組事件為諾如病毒GII.P12_GII.3型的鎮(zhèn)江毒株ZJ111/2015/CHN和ZJ119/2015/CHN,是由加拿大的參考株U02030/Toronto/CA/GII.3和英國的參考株X86557/Lordsdale/UK/GII.4重組而成。結(jié)論 2015年鎮(zhèn)江地區(qū)諾如病毒感染以GII組為主,中青年陽性率最高,新GII.17型變異株為最主要的流行基因型,主要的重組事件為GII.3與GII.4的重組。
[Abstract]:Objective to analyze the infection status and evolutionary recombination of Norovirus in Zhenjiang area in 2015.Methods fecal specimens of diarrhea patients in Zhenjiang sentinel hospital in 2015 were collected, and real-time fluorescence quantitative PCR(q RT-PCR method was used to detect norovirus GI/GII nucleic acid.The Rd RP region and Capsid region of Norovirus gene were amplified by reverse transcription PCR RT-PCR method. Sequence determination, genotyping, sequence evolution and gene recombination analysis were performed by Mega software and RDP software.Results among 832 samples, the positive rate of norovirus was 5.05% / 832% in GII group (4.81% / 832%), accounting for 95.2420 years old, the highest positive rate was in young people aged 10 to 20 years, reaching 25.00% 14% 56%. In 2015, the positive rate of norovirus virus was highest in January, November and second month.The highest percentage of GII.17 type was 63.89%, and the new GII.17 variant was the main epidemic strain.The major recombination events were the Zhenjiang strain of Norovirus GII.P12_GII.3 ZJ111/2015/CHN and ZJ119 / 2015 / CHN, which were recombined from the reference strain U02030/Toronto/CA/GII.3 of Canada and the reference strain X86557/Lordsdale/UK/GII.4 of the United Kingdom.Conclusion the infection of Norovirus in Zhenjiang area in 2015 was mainly in GII group, the positive rate was the highest in young and middle-aged people, the new GII.17 variant was the main prevalent genotype, and the main recombinant event was the recombination of GII.3 and GII.4.
【作者單位】： 鎮(zhèn)江市疾病預(yù)防控制中心;
【基金】：鎮(zhèn)江市衛(wèi)生科技重點專項項目(SHW2015015) 鎮(zhèn)江市科技計劃社會發(fā)展項目(SH2015083)
【分類號】：R440;R512.5

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