本文選題：艾滋病病毒　切入點(diǎn)：耐藥　出處：《中國艾滋病性病》2017年04期 　論文類型：期刊論文

【摘要】：目的了解紹興市1型艾滋病病毒(HIV-1)株的耐藥變異情況。方法分析2013-2014年紹興地區(qū)新確認(rèn)未經(jīng)抗病毒治療的130例HIV-1感染者,以及2014年前抗病毒治療滿一年且病毒未抑制的34例感染者樣本,采用反轉(zhuǎn)錄-聚合酶鏈?zhǔn)椒磻?yīng)(RT-PCR)和巢式-聚合酶鏈?zhǔn)椒磻?yīng)(Nested-PCR)方法擴(kuò)增HIV-1的pol基因區(qū)全長,參照美國斯坦福大學(xué)HIV耐藥數(shù)據(jù)庫,確定HIV亞型及耐藥突變位點(diǎn)。結(jié)果 34例新診斷HIV感染者及33例病毒未抑制感染者的樣品檢測到耐藥突變,主要亞型為CRF01_AE和CRF07_BC。僅在病毒未抑制感染者中檢測到針對核苷類反轉(zhuǎn)錄酶抑制劑(NRTIs)的耐藥突變,主要類型有M184V(42.42%)、K70R(15.15%)、D67N(12.12%)。在新確認(rèn)感染者和病毒未抑制感染者兩組人群中,均檢測到針對非核苷類反轉(zhuǎn)錄酶抑制劑(NNRTIs)耐藥的突變,主要類型有V179D/E/T(10.00%和18.18%)、K103N(2.22%和21.21%);在病毒未抑制感染者中還有Y181CY(21.21%)、V90I(12.12%)、G190A(15.15%)。在反轉(zhuǎn)錄酶(RT)區(qū)和整合酶(IN)區(qū)僅檢測到少量次要耐藥突變。結(jié)論紹興市HIV經(jīng)抗病毒治療后易產(chǎn)生耐藥性,部分可在新感染人群中傳播,應(yīng)對HIV耐藥變異加強(qiáng)監(jiān)測。
[Abstract]:Objective to investigate the variation of drug resistance of HIV-1 strain in Shaoxing city. Methods 130 new cases of HIV-1 infected patients who were not treated with antiviral therapy in Shaoxing from 2013 to 2014 were analyzed. The total length of HIV-1 pol gene was amplified by reverse transcription-polymerase chain reaction (RT-PCR) and nested polymerase chain reaction (Nested-PCR) in 34 infected patients who had been treated with antiviral therapy for more than one year before 2014. According to the HIV database of Stanford University in the United States, the subtype of HIV and the mutation site of drug resistance were determined. Results Drug resistance mutations were detected in 34 newly diagnosed HIV infected patients and 33 uninhibited viral infections. The main subtypes were CRF01_AE and CRF07S BC.The resistant mutations against nucleoside reverse transcriptase inhibitor (NRT Isa) were detected only in those infected with the virus without inhibition. The main types of drug resistance were M1844 VX 42.42TK70RK70RGN 12.120.In the two groups of newly identified and uninhibited infected persons, The mutation of NNRT Isa, a non-nucleoside reverse transcriptase inhibitor, was detected. The main types were V 179D / T / T 10.00% and 18.1818 / K103N 2.22% and 21.21% respectively. Among the infected patients, there were also Y181 CY21. 21. 21 / 21 V90I and 12. 12% G190A0. Only a small number of minor drug resistance mutations were detected in reverse transcriptase (RT) region and integrase inine region. Conclusion HIV is susceptible to drug resistance after antiviral therapy in Shaoxing City, and it is easy to develop drug resistance after antiviral therapy in Shaoxing City. Some of them can be spread in the newly infected population, and the surveillance of drug resistance variation of HIV should be strengthened.
【作者單位】： 紹興市疾病預(yù)防控制中心;
【基金】：紹興市科技局科技計劃(2013B70065)~~
【分類號】：R512.91

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