本文選題：阿片類毒品　切入點：HIV-　出處：《病毒學(xué)報》2015年02期 　論文類型：期刊論文

【摘要】：探討濫用阿片類毒品(Opiates)對Ⅰ型艾滋病病毒(Human immunodeficiency virus,HIV-1)感染者外周血單個核細胞(PBMCs)中TLR9基因表達水平的影響,為闡明阿片類毒品促進HIV-1復(fù)制的作用機制奠定基礎(chǔ)。首先在南寧、柳州、欽州市地區(qū)的美沙酮、艾滋病自愿咨詢檢測門診招募對象,經(jīng)研究對象知情同意,分為4組,即阿片類毒品濫用的HIV-1感染組(Opiates HIV(+)組)、阿片類毒品濫用非HIV-1感染組(Opiates HIV(-)組)、非阿片類毒品濫用的HIV感染組(Non-opiates HIV(+)組)和健康對照組(Control組),每組隨機招募50人。其次,以問卷形式調(diào)查對象的人口學(xué)特征,并采集其外周靜脈血,分離出PBMCs后提取RNA。最后采用qPCR、蛋白質(zhì)印跡法(Western blot,WB)法檢測4組人群PBMCs中TLR9的mRNA、蛋白表達水平。經(jīng)調(diào)查發(fā)現(xiàn)4組人群在年齡、性別、民族、戶籍所在地、婚姻狀況、文化程度和吸毒年限等人口學(xué)特征方面差異均無統(tǒng)計學(xué)意義(P0.05)。Opiates HIV(+)與Non-Opiates HIV(+)組病毒載量中位數(shù)分別為4.450×103和3.977×103 cp/mL,差異有統(tǒng)計學(xué)意義(P0.05)。TLR9 mRNA相對表達量在Opiates HIV(+)、Non-Opiates HIV(+)、Opiates HIV(-)和Control組中分別為(2.13±1.59)×10-3、(3.66±2.22)×10-3、(1.96±1.42)×10-3和(7.66±4.87)×10-3。阿片類毒品濫用和HIV感染對TLR9的表達存在交互作用(F=25.91,P=0.000)。經(jīng)單獨效應(yīng)分析,HIV陽性與陰性人群中,阿片類毒品濫用者TLR9相對表達量均明顯低于非毒品濫用者(P0.05);在吸毒人群中,HIV陽性者與HIV陰性者兩組間差異沒有統(tǒng)計學(xué)意義(P0.05);在不吸毒人群中,HIV陽性者低于正常組,兩組間差異有統(tǒng)計學(xué)意義(P0.05)。WB的結(jié)果顯示,Opiates HIV(+)、Non-Opiates HIV(+)、Opiates HIV(-)三組TLR9蛋白的表達量均低于Control組。上述結(jié)果表明阿片類毒品能夠下調(diào)HIV-1感染者PBMCs中TLR9的表達水平,提示阿片類毒品可能通過影響TLR9所介導(dǎo)的機體免疫效應(yīng),進而促進HIV-1的感染復(fù)制。
[Abstract]:To investigate the effect of opioid drug abuse (Opiateses) on the expression of TLR9 gene in peripheral blood mononuclear cells (PBMCs) of human immunodeficiency virus-1 (HIV-1) infected patients, and to lay a foundation for elucidating the mechanism of opioid drugs promoting HIV-1 replication. Methadone and HIV voluntary counseling and testing outpatient recruitment in Qinzhou area were divided into 4 groups with the informed consent of the subjects studied. The HIV-1 infection group of opioid drug abuse, the Opiates HIV () group, the opioid drug abuse non-#en1# infection group, the non-opioid drug abuse HIV infection group and the healthy control group were recruited at random with 50 persons in each group. The demographic characteristics of the subjects were investigated by questionnaire, and their peripheral venous blood was collected. PBMCs was isolated and extracted. At last, the mRNAs and protein expression levels of TLR9 in PBMCs were detected by qPCRand Western blotWB. the results showed that the four groups were in age, sex, nationality, domicile location and marital status. There was no significant difference in demographic characteristics such as educational level and drug abuse age. The median viral load of Non-Opiates and HIV group were 4.450 脳 10 ~ 3 and 3.977 脳 10 ~ 3 cp / mL, respectively. The difference was statistically significant in the relative expression of mRNA between Opiates non-Opiates and Opiates (Non-Opiates). In the Control group, 2.13 鹵1.59) 脳 10-3 (3.66 鹵2.22) 脳 10-3) 1.96 鹵1.42) 脳 10-3 and 7.66 鹵4.87) 脳 10-3 respectively. There was interaction between opioid drug abuse and HIV infection on the expression of TLR9. The relative expression of TLR9 in opioid drug abusers was significantly lower than that in non-drug abusers (P 0.05); there was no significant difference between the two groups in TLR9 positive and HIV negative in drug users; in non-drug abusers, the level of TLR9 positive was lower than that in the normal group. There was significant difference between the two groups in the expression of TLR9 protein in the three groups of Control. These results indicated that opioid drugs could down-regulate the expression of TLR9 in PBMCs of HIV-1 infected patients. The results suggest that opioid drugs may promote the replication of HIV-1 by affecting the immune effect mediated by TLR9.
【作者單位】： 廣西醫(yī)科大學(xué)公共衛(wèi)生學(xué)院 廣西艾滋病防治研究重點實驗室;廣西壯族自治區(qū)疾病預(yù)防控制中心消殺與媒介控制所;廣西醫(yī)科大學(xué)第一附屬醫(yī)院老年消化內(nèi)科;
【基金】：國家自然科學(xué)基金資助項目(81171624) 教育部博導(dǎo)課題(20114503110003) 廣西醫(yī)科大學(xué)青年科學(xué)基金項目(GXMUYSF201210)
【分類號】：R512.91
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本文編號：1594193