發(fā)布時間：2018-02-19 18:28

  本文關(guān)鍵詞： 血吸蟲 日本 巢式PCR 哨鼠　出處：《中國病原生物學(xué)雜志》2015年04期 　論文類型：期刊論文

【摘要】：目的建立巢式PCR法用以輔助日本血吸蟲易感水域中哨鼠的監(jiān)測。方法選擇日本血吸蟲基因組重復(fù)序列DNA SjR2(2456~3171bp)作為外道PCR的區(qū)域,其中2719~3019bp作為內(nèi)道PCR的擴增區(qū)域。以不同稀釋度的日本血吸蟲成蟲基因組DNA為模板進行敏感性測試,并對未感染和感染10條、40條尾蚴的小鼠血清進行檢測。將巢式PCR法用于2013年8個哨鼠監(jiān)測點的監(jiān)測,觀察其應(yīng)用價值。結(jié)果巢式PCR中內(nèi)道引物擴增產(chǎn)物為301bp,血吸蟲基因組DNA濃度低至10-5ng/μl時,仍擴增陽性;以10條和40條尾蚴感染4周的小鼠血清DNA為模板進行巢式PCR,結(jié)果為陽性。將巢式PCR用于2013年血吸蟲易感水域小范圍哨鼠的監(jiān)測,第2組的20只小鼠中有2只陽性,但解剖小鼠未檢出蟲體。結(jié)論巢式PCR法可輔助血吸蟲易感水域中哨鼠的監(jiān)測,特別對感染度較低水域的監(jiān)測有一定幫助。
[Abstract]:Objective to establish a nested PCR method to assist sentinel surveillance in susceptible waters of schistosomiasis japonicum. Methods the genomic repeat sequence of Schistosoma japonicum DNA SjR2C24563171bp) was selected as the outer channel PCR region. Among them, 2719 / 3019bp was used as the amplified region of internal PCR. The genomic DNA of Schistosoma japonicum with different dilution was used as a template for sensitivity test. The sera of 10 uninfected and infected mice with 40 cercariae were detected. The nested PCR method was used to monitor 8 sentinel mouse surveillance sites in 2013. Results the PCR products of nested PCR were 301 BP and the genomic DNA concentration of Schistosoma japonicum was as low as 10 ~ (-5) ng / 渭 l. Using 10 cercariae and 40 cercariae infected mice serum DNA as a template, the results were positive. In 2013, the nested PCR was used to monitor the sentinel mice in the susceptible area of schistosomiasis, 2 of the 20 mice in the second group were positive. Conclusion the nested PCR method can assist the surveillance of sentinel rats in susceptible areas of schistosomiasis, especially in the low infection areas.
【作者單位】： 南京醫(yī)科大學(xué)病原生物學(xué)系;衛(wèi)生部重要寄生蟲病預(yù)防與控制高技術(shù)重點實驗室江蘇省血吸蟲病防治研究所;
【基金】：國家十一五科技重大專項(No.2008ZX10004-011) 國家十二五科技重大專項(No.2012ZX10004220)
【分類號】：R532.21

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本文編號：1517816