本文關鍵詞： 艾滋病病毒新發(fā)感染 金標銀染免疫滲濾快速檢測方法 限制性抗原親和力方法 BED捕獲酶聯(lián)免疫法 抗病毒治療　出處：《中國艾滋病性病》2017年06期 　論文類型：期刊論文

【摘要】：目的初步評價艾滋病病毒Ⅰ型(HIV-1)新發(fā)感染快速檢測方法,在橫斷面現(xiàn)場調查人群樣本中的應用能力,并分析抗病毒治療對該方法的影響。方法應用該研究前期構建的金標銀染免疫滲濾快速檢測方法(簡稱快速檢測法),檢測2014年江西省國家級哨點新報告的HIV-1感染者和抗病毒治療者的樣本。將新發(fā)感染判定的結果與限制性抗原親和力方法(LAg Avidity EIA,簡稱LAg法)、BED捕獲酶免疫法(BED CEIA,簡稱BED法)進行一致性和相關性比較,進一步分析三種方法檢測抗病毒治療者樣本的差異。結果納入研究的新發(fā)感染者樣本共122份,快速檢測法與LAg法、BED法判定新發(fā)感染結果的一致率分別為94.26%和88.52%。快速檢測法檢測探針灰度值與LAg法和BED法的ODn值具有較高的相關性。R2分別為0.947 5與0.923 4。經(jīng)配對卡方檢驗分析,快速檢測法判定新發(fā)感染的比例顯著低于BED法(P0.001),而與LAg法差異無統(tǒng)計學意義(P=0.257)。納入研究的抗病毒治療者的樣本共117份,快速檢測法對其誤判為近期的比率為16.24%(19/117),而BED方法和LAg方法的誤判比率分別為16.24%(19/117)、14.53%(17/117)。另外,快速檢測法對所有樣本常規(guī)HIV抗體檢測結果均正確。結論金標銀染免疫滲濾快速檢測方法檢測HIV-1新發(fā)感染具有良好的準確性和實際應用性。但抗病毒治療對快速檢測法影響較大,應在橫斷面人群調查時刪除抗病毒治療樣本。
[Abstract]:Objective to evaluate the ability of rapid detection of HIV type I (HIV-1) infection in a cross-sectional survey of population samples. The effect of antiviral therapy on this method was analyzed. Methods the rapid detection method of gold labeled silver staining immunofiltration was used in the early stage of the study. In 2014, the samples of newly reported HIV-1 infected persons and antiviral therapists in Jiangxi Province were detected. The results of the new infection determination were compared with the restrictive antigen affinity method (. LAg Avidity EIA. The consistency and correlation between the two methods were compared by LAg method and BED method. Further analysis of the three methods to detect the difference of antiviral treatment samples. Results A total of 122 newly infected individuals were included in the study, rapid detection method and LAg method. The results of BED were 94.26% and 88.52, respectively. The gray value of probe detected by rapid detection method had a high correlation with the ODn value of LAg method and BED method. 2 = 0.947 respectively. 5 and 0.923 4. Analysis by paired chi-square test. The proportion of new infections detected by rapid detection method was significantly lower than that by BED method (P 0.001). However, there was no significant difference between the two methods (P < 0. 257). A total of 117 samples of antiviral therapy were included in the study. The recent rate of miscalculation by the rapid detection method was 16.24 to 19 / 117, while that of the BED and LAg methods was 16.2419 / 117, respectively. 14.53 / 17 / 117. Conclusion the rapid detection method of gold standard silver staining immunofiltration has good accuracy and practical application in detecting new HIV-1 infection, but it is resistant to disease. Toxic therapy has great influence on rapid detection. Antiviral treatment samples should be removed from cross-sectional population surveys.
【作者單位】： 中國疾病預防控制中心性病艾滋病預防控制中心參比室;河北醫(yī)科大學;江西省疾病預防控制中心;首都醫(yī)科大學附屬北京佑安醫(yī)院;北京金豪制藥股份有限公司;
【基金】：北京市科委課題(D161100000416001)~~
【分類號】：R512.91
【正文快照】： Supported by Beijing Science and Technology Project(D161100000416001)艾滋病病毒(HIV)新發(fā)感染率是評價艾滋病(AIDS)流行趨勢和防治措施的重要公共衛(wèi)生指標[1-2],目前普遍采用簡便經(jīng)濟的實驗室方法從橫斷面樣本中檢測新發(fā)感染來進行估算。隨著實驗技術的進步,現(xiàn)在已有20

【相似文獻】

相關期刊論文 前3條

1 孫獻武;美推出艾滋病快速檢測法[J];解放軍健康;1997年03期

2 鄭鈴,詹麗欽,陳貽鍇;丙型肝炎病毒抗體一步快速檢測法及與ELISA的比較[J];中華微生物學和免疫學雜志;1998年05期

3 ;[J];;年期

，

本文編號：1485721