本文關(guān)鍵詞： 艾滋病病毒新發(fā)感染 金標(biāo)銀染免疫滲濾快速檢測(cè)方法 限制性抗原親和力方法 BED捕獲酶聯(lián)免疫法 抗病毒治療　出處：《中國(guó)艾滋病性病》2017年06期 　論文類(lèi)型：期刊論文

【摘要】：目的初步評(píng)價(jià)艾滋病病毒Ⅰ型(HIV-1)新發(fā)感染快速檢測(cè)方法,在橫斷面現(xiàn)場(chǎng)調(diào)查人群樣本中的應(yīng)用能力,并分析抗病毒治療對(duì)該方法的影響。方法應(yīng)用該研究前期構(gòu)建的金標(biāo)銀染免疫滲濾快速檢測(cè)方法(簡(jiǎn)稱(chēng)快速檢測(cè)法),檢測(cè)2014年江西省國(guó)家級(jí)哨點(diǎn)新報(bào)告的HIV-1感染者和抗病毒治療者的樣本。將新發(fā)感染判定的結(jié)果與限制性抗原親和力方法(LAg Avidity EIA,簡(jiǎn)稱(chēng)LAg法)、BED捕獲酶免疫法(BED CEIA,簡(jiǎn)稱(chēng)BED法)進(jìn)行一致性和相關(guān)性比較,進(jìn)一步分析三種方法檢測(cè)抗病毒治療者樣本的差異。結(jié)果納入研究的新發(fā)感染者樣本共122份,快速檢測(cè)法與LAg法、BED法判定新發(fā)感染結(jié)果的一致率分別為94.26%和88.52%。快速檢測(cè)法檢測(cè)探針灰度值與LAg法和BED法的ODn值具有較高的相關(guān)性。R2分別為0.947 5與0.923 4。經(jīng)配對(duì)卡方檢驗(yàn)分析,快速檢測(cè)法判定新發(fā)感染的比例顯著低于BED法(P0.001),而與LAg法差異無(wú)統(tǒng)計(jì)學(xué)意義(P=0.257)。納入研究的抗病毒治療者的樣本共117份,快速檢測(cè)法對(duì)其誤判為近期的比率為16.24%(19/117),而B(niǎo)ED方法和LAg方法的誤判比率分別為16.24%(19/117)、14.53%(17/117)。另外,快速檢測(cè)法對(duì)所有樣本常規(guī)HIV抗體檢測(cè)結(jié)果均正確。結(jié)論金標(biāo)銀染免疫滲濾快速檢測(cè)方法檢測(cè)HIV-1新發(fā)感染具有良好的準(zhǔn)確性和實(shí)際應(yīng)用性。但抗病毒治療對(duì)快速檢測(cè)法影響較大,應(yīng)在橫斷面人群調(diào)查時(shí)刪除抗病毒治療樣本。
[Abstract]:Objective to evaluate the ability of rapid detection of HIV type I (HIV-1) infection in a cross-sectional survey of population samples. The effect of antiviral therapy on this method was analyzed. Methods the rapid detection method of gold labeled silver staining immunofiltration was used in the early stage of the study. In 2014, the samples of newly reported HIV-1 infected persons and antiviral therapists in Jiangxi Province were detected. The results of the new infection determination were compared with the restrictive antigen affinity method (. LAg Avidity EIA. The consistency and correlation between the two methods were compared by LAg method and BED method. Further analysis of the three methods to detect the difference of antiviral treatment samples. Results A total of 122 newly infected individuals were included in the study, rapid detection method and LAg method. The results of BED were 94.26% and 88.52, respectively. The gray value of probe detected by rapid detection method had a high correlation with the ODn value of LAg method and BED method. 2 = 0.947 respectively. 5 and 0.923 4. Analysis by paired chi-square test. The proportion of new infections detected by rapid detection method was significantly lower than that by BED method (P 0.001). However, there was no significant difference between the two methods (P < 0. 257). A total of 117 samples of antiviral therapy were included in the study. The recent rate of miscalculation by the rapid detection method was 16.24 to 19 / 117, while that of the BED and LAg methods was 16.2419 / 117, respectively. 14.53 / 17 / 117. Conclusion the rapid detection method of gold standard silver staining immunofiltration has good accuracy and practical application in detecting new HIV-1 infection, but it is resistant to disease. Toxic therapy has great influence on rapid detection. Antiviral treatment samples should be removed from cross-sectional population surveys.
【作者單位】： 中國(guó)疾病預(yù)防控制中心性病艾滋病預(yù)防控制中心參比室;河北醫(yī)科大學(xué);江西省疾病預(yù)防控制中心;首都醫(yī)科大學(xué)附屬北京佑安醫(yī)院;北京金豪制藥股份有限公司;
【基金】：北京市科委課題(D161100000416001)~~
【分類(lèi)號(hào)】：R512.91
【正文快照】： Supported by Beijing Science and Technology Project(D161100000416001)艾滋病病毒(HIV)新發(fā)感染率是評(píng)價(jià)艾滋病(AIDS)流行趨勢(shì)和防治措施的重要公共衛(wèi)生指標(biāo)[1-2],目前普遍采用簡(jiǎn)便經(jīng)濟(jì)的實(shí)驗(yàn)室方法從橫斷面樣本中檢測(cè)新發(fā)感染來(lái)進(jìn)行估算。隨著實(shí)驗(yàn)技術(shù)的進(jìn)步,現(xiàn)在已有20

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