本文關(guān)鍵詞： 艾滋病病毒 未治療病人 耐藥基因突變 耐藥警戒線調(diào)查方法　出處：《中國艾滋病性病》2017年08期 　論文類型：期刊論文

【摘要】：目的對溫州地區(qū)未治療艾滋病(AIDS)病人體內(nèi)艾滋病病毒(HIV)毒株耐藥基因的突變情況進行調(diào)查分析,了解該地區(qū)AIDS病人的本底耐藥情況。方法對50例未接受抗病毒治療的AIDS病人HIVpol區(qū)的蛋白酶(PR)全長和部分反轉(zhuǎn)錄酶(RT)基因進行擴增、脫氧核糖核酸(DNA)測序、亞型鑒定,并與國際HIV耐藥數(shù)據(jù)庫比對分析。結(jié)果共獲得38份有效序列,根據(jù)pol區(qū)亞型分析發(fā)現(xiàn),CRF01_AE占36.8%(14例),CRF07_BC重組占36.8%(14例),C亞型占18.4%(7例),B亞型占7.89%(3例)。未檢出針對蛋白酶抑制劑(PIs)、核苷類反轉(zhuǎn)錄酶抑制劑(NRTIs)的耐藥病例。針對非核苷類反轉(zhuǎn)錄酶抑制劑(NNRTIs)檢出3例耐藥病例,突變類型分別為V179D、E138A和K103N。同時,2015年溫州HIV耐藥毒株的傳播水平5%,處于低傳播水平。結(jié)論對于未經(jīng)抗病毒治療組,耐藥基因型以CRF01_AE重組、BC重組為主,HIV耐藥毒株在溫州仍處于低度傳播水平,發(fā)現(xiàn)一個重要耐藥基因突變位點是K103N。
[Abstract]:The purpose of the Wenzhou area is not the treatment of AIDS (AIDS) in patients with AIDS virus (HIV) mutation strains of resistant genes were investigated, understand the bottom resistance of AIDS patients in this area. Methods of protein enzyme in 50 cases did not receive antiviral treatment in patients with AIDS HIVpol region (PR) gene and part reverse transcriptase (RT) gene was amplified, deoxyribonucleic acid (DNA) subtype identification, sequencing, and analysis and international HIV resistance database. Results 38 valid sequences were obtained, according to the pol genotype analysis showed that CRF01_AE accounted for 36.8% (14 cases), recombinant CRF07_BC accounted for 36.8% (14 cases), C subtype 18.4% (7 cases), B subtype accounted for 7.89% (3 cases). Not detected against protease inhibitors (PIs), nucleoside reverse transcriptase inhibitors (NRTIs) resistance. In the case of non nucleoside reverse transcriptase inhibitor (NNRTIs) were detected in 3 cases of drug resistant cases, mutation types were V179D, E138A and K1 03N. at the same time, the 2015 Wenzhou HIV resistant strains spread level 5%, at a low level. Conclusion for communication without antiviral treatment group, antibiotic resistance genes by recombinant CRF01_AE, BC recombinant, HIV is still in the low level of resistant strains spread in Wenzhou, found an important resistance gene mutation site is K103N.

【作者單位】： 溫州市疾病預(yù)防控制中心;
【基金】：溫州市科技計劃項目(Y20160503)~~
【分類號】：R512.91
【正文快照】： 高效抗反轉(zhuǎn)錄病毒治療(HAART)可降低艾滋病病毒(HIV)感染者體內(nèi)的病毒載量,恢復(fù)部分免疫功能,不過長期接受HAART加上病人依從性差,會引起耐藥[1]。HIV感染者在接受HAART之后,其攜帶的耐藥毒株可能會發(fā)展成為優(yōu)勢株。一旦該情況發(fā)生,就有可能將攜帶耐藥突變的毒株傳播到未經(jīng)治

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