本文關鍵詞： 細粒棘球蚴病 免疫球蛋白G亞型 受試者工作特征曲線 囊液　出處：《中國寄生蟲學與寄生蟲病雜志》2015年02期 　論文類型：期刊論文

【摘要】：目的用細粒棘球蚴囊液抗原檢測甘肅省環(huán)縣地區(qū)細粒棘球蚴病患者血清中特異性Ig G及其亞型抗體。方法采集甘肅省環(huán)縣地區(qū)經B超確診的37例細粒棘球蚴病患者和29例健康人血清,ELISA法檢測血清中抗棘球蚴囊液抗原的特異性Ig G抗體及其亞型Ig G1、Ig G2和Ig G4。應用Med Calc軟件,以B超檢測結果為金標準,根據ELISA結果繪制受試者工作特征(ROC)曲線,通過曲線下面積的配對z檢驗,比較這4種抗體的診斷性能差異,確定最佳診斷閾值。用卡方檢驗分析并比較棘球蚴囊液抗原用于檢測4種抗體的靈敏性和特異性。結果用囊液抗原檢測Ig G、Ig G1、Ig G2和Ig G4抗體的ROC曲線下面積分別為0.722、0.919、0.712和0.835,其中Ig G1的曲線下面積顯著大于Ig G和Ig G2的面積(P0.05)。棘球蚴囊液抗原檢測這4種抗體的靈敏性分別為54.1%、91.9%、67.6%和75.7%,其中Ig G1的靈敏性高于Ig G、Ig G2和Ig G4(P0.05);特異性分別為89.7%、82.8%、72.4%和89.7%,Ig G1的特異性與Ig G、Ig G2和Ig G4相比,差異無統(tǒng)計學意義(P0.05)。檢測Ig G4抗體在CEⅠ-Ⅲ型病例中的靈敏性高于CEⅣ-Ⅴ型病例(P0.05)。結論棘球蚴囊液抗原檢測血清中Ig G1抗體的靈敏性優(yōu)于其他3種抗體,但特異性與其他3種抗體間差異無統(tǒng)計學意義。
[Abstract]:Objective to detect specific Ig in sera of patients with echinococcosis in Huanxian district of Gansu province by using hydatid fluid antigen of hydatid granulosus (Echinococcus granulosus). Methods Serum samples from 37 patients with hydatid granulosus and 29 healthy persons were collected from Huanxian district of Gansu province. The specific IgG antibody and its subtype Ig G 2 and IgG G 4 were detected by ELISA. Med Calc software was used. According to the results of B-ultrasound as the gold standard, according to the ELISA results, the operating characteristics of the subjects were drawn. The diagnostic performance of the four antibodies was compared by the paired z test of the area under the curve. The best diagnostic threshold was determined. The sensitivity and specificity of the four antibodies were analyzed and compared by chi-square test. The areas under the ROC curve of IgG G2 and IgG G4 antibodies were 0.722 ~ 0.919 ~ 0.712 and 0.835, respectively. The area under the curve of IgG1 was significantly larger than that of IgG and IgG2.The sensitivity of Echinococcus cyst fluid antigen in detecting the four antibodies was 54.1%, respectively. The sensitivity of Ig G 1 was higher than that of IgG G 2 and G 4 P 0.05; The specificity of Ig G1 was 82.7% and 89.7%, respectively. The specificity of Ig G 1 was higher than that of IgG G 2 and G 4. The sensitivity of detecting IgG4 antibody in CE 鈪，

本文編號：1471750