發(fā)布時(shí)間：2018-01-26 08:19

  本文關(guān)鍵詞： 隱匿性乙型肝炎病毒感染 HBsAg 抗-HBc 獻(xiàn)血者 核酸擴(kuò)增技術(shù)　出處：《中國(guó)輸血雜志》2017年07期 　論文類(lèi)型：期刊論文

【摘要】：目的研究分析嘉興地區(qū)獻(xiàn)血人群隱匿性乙肝病毒(OBI)感染血清學(xué)及病毒學(xué)特征。方法采用常規(guī)的ELISA(HBsAg、抗-HCV、抗-HIV、抗-TP)和核酸擴(kuò)增技術(shù)(NAT)對(duì)本中心52 698份無(wú)償獻(xiàn)血者標(biāo)本進(jìn)行聯(lián)合篩查,對(duì)NAT+標(biāo)本做進(jìn)一步鑒別檢測(cè)病毒類(lèi)型;收集HBs Ag-/HBV-DNA+的標(biāo)本再另選3種不同的HBs Ag酶免試劑盒定性檢測(cè),并選用化學(xué)發(fā)光對(duì)HBsAg和抗-HBs定量檢測(cè),同時(shí)采用實(shí)時(shí)熒光PCR(QPCR)進(jìn)行HBV核酸病毒載量測(cè)定;再結(jié)合血清學(xué)乙肝三系標(biāo)志物、追蹤檢測(cè)和一般流行病統(tǒng)計(jì)學(xué)資料(性別、獻(xiàn)血次數(shù)和年齡)來(lái)進(jìn)一步分析研究OBI的血清學(xué)及病毒學(xué)相關(guān)分布情況。結(jié)果共確認(rèn)47例OBI感染者,OBI流行率為0.89‰(1∶1 121),窗口期(WP)2例(1∶26 349);HBsAg、HBeAg檢測(cè)結(jié)果均為陰性,發(fā)現(xiàn)6種OBI血清學(xué)模式,抗-HBs定量100 m IU/m L的標(biāo)本占27.66%(13/47),抗-HBc+占91.49%(43/47),HBV-DNA核酸定量范圍(4.10-1.82)×10~3(IU/m L)(中位數(shù)15.83),5例陽(yáng)性對(duì)照HBsAg+/HBV-DNA+病毒載量范圍(61.47-1.28)×104(IU/m L)(中位數(shù)538.15),兩組結(jié)果比較,其差異有統(tǒng)計(jì)學(xué)意義(P0.05);40歲以上的男性獻(xiàn)血者OBI感染率高(P0.05),多次獻(xiàn)血者與首次獻(xiàn)血者OBI感染率差異有統(tǒng)計(jì)學(xué)意義(0.01P0.05)。結(jié)論 OBI感染者病毒載量低,以抗-HBc+為主要血清學(xué)表現(xiàn)形式;NAT可以檢出OBI,縮短窗口期,有利于保障臨床血液安全。
[Abstract]:Objective to study the serological and virological characteristics of occult hepatitis B virus (OBI) infection among blood donors in Jiaxing. Methods routine Elisa HBsAg, anti-HCV and anti-HIV were used. Anti-TP-) and nucleic acid amplification technique (Nat) were used to screen 52 698 volunteer blood donor samples in our center, and NAT samples were further identified and tested for virus types. The samples of HBsAg-/-r HBV-DNA were collected and three different kinds of HBsAg enzyme immunoassay kits were selected for qualitative detection, and chemiluminescence was used to detect HBsAg and anti-HBs quantitatively. At the same time, the viral load of HBV nucleic acid was determined by real-time fluorescence PCR. Combined with serological hepatitis B three series markers, tracking detection and general epidemiological statistical data (gender). To further analyze the serological and virological distribution of OBI. Results 47 cases of OBI infection were confirmed. The prevalence rate of OBI was 0.89 鈥，

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