本文關(guān)鍵詞：廣東省鼠傷寒沙門菌的分子流行病學(xué)研究　出處：《南方醫(yī)科大學(xué)》2014年碩士論文　論文類型：學(xué)位論文

  更多相關(guān)文章： 鼠傷寒沙門菌 加強(qiáng)監(jiān)測 耐藥譜 脈沖場凝膠電泳(Pulse-Field Gel Electrophoresis PFGE) 多位點(diǎn)可變數(shù)目串聯(lián)重復(fù)序列分析(Multiple-locus Variable-numbers Tandem Repeat Analysis MLVA)

【摘要】：研究背景 食源性疾病是各種致病因子通過攝食進(jìn)入人體內(nèi)引起的一類感染性或中毒性疾病,每年全球僅食源性腹瀉就造成幾百萬兒童死亡,即使在發(fā)達(dá)國家也至少有三分之一的人患食源性疾病,沙門菌(Salmonella)是主要的病原體之一。隨著生產(chǎn)生活模式的改變,地域之間人員、食品往來、物資流通更加廣泛,食品加工、供給及飲食方式的多樣化,每年世界范圍包括我國在內(nèi)都會(huì)有沙門菌引起的食源性疾病報(bào)道,所造成的公共衛(wèi)生問題不容忽視,衛(wèi)生經(jīng)濟(jì)負(fù)擔(dān)逐漸引起相關(guān)部門的重視。沙門菌有2500多個(gè)血清型,其中鼠傷寒(Salmonella enterica serovar Typhimurium, S. Typhimurium)是引起人類胃腸炎或食物中毒最常見,最重要的血清型之一,寄生于人體和爬行類動(dòng)物的腸道,是一種重要的人畜共患病原菌,臨床主要表現(xiàn)為胃腸炎型、敗血癥等沙門菌病,由該菌引起的食源性疾病暴發(fā)在國內(nèi)外經(jīng)常發(fā)生,并造成重大的經(jīng)濟(jì)損失。 廣東省每年都會(huì)有鼠傷寒沙門菌引起的食物中毒事件報(bào)道,腹瀉暴發(fā)事件由以前的點(diǎn)源性集中暴發(fā),越來越多地轉(zhuǎn)變?yōu)榭绲貐^(qū)的“散在暴發(fā)”,使污染源及傳播途經(jīng)的發(fā)現(xiàn)越來越依賴于分子分型手段的輔助及確認(rèn)。目前已有比較成熟的國際通用標(biāo)準(zhǔn)化脈沖場凝膠電泳(Pulse-Field Gel Electrophoresis, PFGE)分型方案、第二代分子分型技術(shù)多位點(diǎn)可變數(shù)目串聯(lián)重復(fù)序列分析(Multiple-locus Variable-numbers Tandem Repeat Analysis, MLVA)以及英國華威大學(xué)公布的7個(gè)管家基因位點(diǎn)的多位點(diǎn)序列分析(Multiple Locus Sequence Typing, MLST)分型方法,并成功應(yīng)用于多起由鼠傷寒沙門菌引起的食源性疾病暴發(fā)的溯源調(diào)查。為了建立以實(shí)驗(yàn)室為基礎(chǔ)的主動(dòng)監(jiān)測系統(tǒng),完善分子分型網(wǎng)絡(luò)庫,廣東省自2009年正式啟動(dòng)沙門菌加強(qiáng)監(jiān)測(Enhance Salmonella Surveillance, ESS),對(duì)散發(fā)的腹瀉患者進(jìn)行監(jiān)測,通過監(jiān)測發(fā)現(xiàn)鼠傷寒沙門菌為本省非傷寒沙門菌感染性腹瀉病例的優(yōu)勢菌型。 本研究旨在廣東地區(qū)開展鼠傷寒沙門菌分子分型監(jiān)測研究,對(duì)2009-2011年ESS的鼠傷寒沙門菌臨床分離株進(jìn)行PFGE和MLVA分子分型,以獲取菌株的核酸指紋基礎(chǔ)數(shù)據(jù),并分析本省監(jiān)測點(diǎn)醫(yī)院腹瀉病例臨床分離株之間的遺傳學(xué)關(guān)聯(lián)性,了解其分子遺傳特征,為發(fā)現(xiàn)鼠傷寒沙門菌可能引起的暴發(fā)提供本底發(fā)病水平,為實(shí)驗(yàn)室監(jiān)測、鑒定溯源及防控預(yù)警提供技術(shù)支持和數(shù)據(jù)參考,科學(xué)防控鼠傷寒沙門菌所致的食源性疾病提供決策依據(jù),提高食源性疾病的檢測和防控能力。 研究目的 1、了解廣東省2009-2011年沙門菌加強(qiáng)監(jiān)測系統(tǒng)的現(xiàn)狀,主要分析優(yōu)勢血清型鼠傷寒的流行病學(xué)特征、抗生素敏感性和多重耐藥的特點(diǎn)。 2、對(duì)三年加強(qiáng)監(jiān)測收集的鼠傷寒沙門菌進(jìn)行PFGE分型,對(duì)菌株帶型的聚集和分布規(guī)律進(jìn)行分析,以發(fā)現(xiàn)帶型的流行病學(xué)規(guī)律,為監(jiān)測和暴發(fā)研究提供基礎(chǔ)的分子分型數(shù)據(jù)庫。 3、參考PulseNet公布的鼠傷寒沙門菌MLVA分型標(biāo)準(zhǔn)方案,進(jìn)行相關(guān)地優(yōu)化,并將其應(yīng)用于加強(qiáng)監(jiān)測系統(tǒng)的分型分析,對(duì)MLVA型別的親緣進(jìn)化關(guān)系和分布規(guī)律進(jìn)行分析,并與PFGE分型進(jìn)行比較,綜合評(píng)價(jià)MLVA的分型能力,以闡明該方法實(shí)際應(yīng)用的價(jià)值和意義,實(shí)現(xiàn)簡便、快速的分型技術(shù)提高實(shí)驗(yàn)室主動(dòng)監(jiān)測能力的目標(biāo)。 研究方法 1、采用WHO推薦的改良K-B紙片擴(kuò)散法對(duì)廣東省2009-2011年ESS的鼠傷寒沙門菌進(jìn)行抗生素敏感性實(shí)驗(yàn)。根據(jù)美國2012版CLSI選擇12種抗生素紙片和判讀敏感性結(jié)果,最后使用WHONET5.4進(jìn)行抗生素敏感性試驗(yàn)數(shù)據(jù)分析。菌株的流行病學(xué)資料和檢測情況錄入Excel2003,并將數(shù)值導(dǎo)入SPSS13.0軟件進(jìn)行統(tǒng)計(jì)分析,對(duì)2009-2011年加強(qiáng)監(jiān)測系統(tǒng)沙門菌的陽性檢出率狀況,優(yōu)勢血清型鼠傷寒的地區(qū)、時(shí)間及人群分布特點(diǎn),以及抗生素敏感性和多重耐藥的特點(diǎn)進(jìn)行描述性統(tǒng)計(jì)分析。 2、參照國際PulseNet的沙門菌PFGE分子分型標(biāo)準(zhǔn)操作方案,對(duì)上述沙門菌的優(yōu)勢血清型鼠傷寒進(jìn)行分子分型。菌株先經(jīng)限制性內(nèi)切酶消化,脈沖場凝膠電泳后,使用凝膠成像分析系統(tǒng)生成圖像條帶,BioNumerics軟件對(duì)圖像條帶進(jìn)行識(shí)別、處理,H9812菌株作為標(biāo)準(zhǔn)分子量進(jìn)行校準(zhǔn)。選擇非加權(quán)配對(duì)算術(shù)平均法進(jìn)行條帶的聚類分析,根據(jù)分析結(jié)果使用統(tǒng)一命名規(guī)則對(duì)條帶進(jìn)行編號(hào),建立沙門菌加強(qiáng)監(jiān)測的PFGE分子分型數(shù)據(jù)庫。結(jié)合菌株的流行病學(xué)資料,使用描述性分析歸納帶型的聚集和分布規(guī)律,主要是三間分布規(guī)律；并對(duì)優(yōu)勢帶型的三間分布差異進(jìn)行卡方檢驗(yàn),同時(shí)結(jié)合耐藥譜型進(jìn)行關(guān)聯(lián)性分析。 3、參考PulseNet推薦的7個(gè)位點(diǎn)MLVA標(biāo)準(zhǔn)操作方案,對(duì)反應(yīng)體系和檢測步驟進(jìn)行相關(guān)的優(yōu)化,并將其應(yīng)用于加強(qiáng)監(jiān)測系統(tǒng)鼠傷寒沙門菌的分型分析。使用ABI3730XL測序儀進(jìn)行PCR產(chǎn)物片段大小的檢測,GeneMapper軟件對(duì)片段大小對(duì)應(yīng)的毛細(xì)管熒光信號(hào)進(jìn)行識(shí)別,參照普通瓊脂糖電泳的結(jié)果,對(duì)片段大小進(jìn)行確認(rèn),計(jì)算相應(yīng)位點(diǎn)的重復(fù)數(shù),BioNumerics軟件對(duì)重復(fù)數(shù)進(jìn)行最小生成樹分析,根據(jù)分析結(jié)果使用既定命名規(guī)則對(duì)MLVA型別進(jìn)行編號(hào),建立沙門菌加強(qiáng)監(jiān)測的MLVA分子分型數(shù)據(jù)庫。結(jié)合菌株的流行病學(xué)資料,對(duì)MLVA型別的親緣進(jìn)化關(guān)系、分布規(guī)律以及與耐藥譜的關(guān)系進(jìn)行分析,同時(shí)與PFGE分型進(jìn)行比較,綜合評(píng)價(jià)MLVA的分型能力和實(shí)際應(yīng)用價(jià)值。 研究結(jié)果 1、三年監(jiān)測收集總的樣本數(shù)為19895份,沙門菌的年均檢出率為4.03%(802/19895),鼠傷寒沙門菌的年均檢出率、構(gòu)成比分別為1.38%(275/19895)、34.29%(275/802),鼠傷寒為廣東省沙門菌的優(yōu)勢血清型。275株鼠傷寒沙門菌,以2010年的構(gòu)成比最大(136/275,44.88%),每年的5月、9月為發(fā)病高峰,男女比例為1.57：1,不同年份性別間差異無統(tǒng)計(jì)學(xué)意義(P0.05),0-1歲的嬰幼兒為主要發(fā)病人群(157/275,57.09%),各年份不同年齡段差異有統(tǒng)計(jì)學(xué)意義(P0.05),主要來源于廣州(95/275,34.55%)、中山(59/275,21.45%)等珠三角城市群。 2、275株鼠傷寒沙門菌抗生素敏感性試驗(yàn)結(jié)果顯示,對(duì)頭孢類抗生素敏感性高,均為80%以上,而對(duì)環(huán)丙沙星耐藥率最低(6.2%),對(duì)其余8種抗生素高度耐藥,耐藥率在68.4%-90.5%之間；除了環(huán)丙沙星、鏈霉素,各年份的抗生素耐藥率變化趨勢一致,不同年份的菌株對(duì)氨芐西林、萘啶酸、磺胺甲嗯唑和四環(huán)素耐藥率差異均有統(tǒng)計(jì)學(xué)意義(P0.05)；3株鼠傷寒沙門菌(編號(hào)ESS221.463.797)對(duì)所檢測的12種抗生素全部耐受,255株鼠傷寒為多重耐藥株(92.7％),AMP+CHL+NAL+SMX+STR+TCY+TMP+GEN為廣東省最主要的多重耐藥譜型(104/275,37.8%)。 3、275株鼠傷寒沙門菌經(jīng)Xba I酶切電泳后,相似值為56.09%-100%,分為124種帶型、7大聚類簇,分型D值為0.9286,每種帶型包含1-71株菌株,平均每種帶型有2.22株菌。30種帶型在不同地區(qū)交叉出現(xiàn),17種帶型在不同年份交叉出現(xiàn)。優(yōu)勢帶型為JPXX01.GD0004共71株,主要包括廣州21株(29.6%)、中山17株(23.9%)、東莞14株(19.7%),主要集中于2010年(51/71,71.8%),5月(14/71,19.72%)、9月(11/71,15.49%)為高峰期,以0-1歲男嬰為主,優(yōu)勢條帶的分布符合菌株的流行特征,不同地區(qū)、年代、性別以及年齡組間的分布差異有統(tǒng)計(jì)學(xué)意義(P0.05)；優(yōu)勢帶型主要對(duì)5-9種抗生素耐藥,帶型JPXX01.GD0035和JPXX01.GD0047的耐藥譜為AMP+CHL+NAL+SMX+STR+TCY+TMP+GEN. 4、275株鼠傷寒沙門菌的MLVA分型結(jié)果顯示,所有菌株分為143種型別、5個(gè)親緣進(jìn)化群,D值為0.9665,每種型別有1-42株菌株,平均每種帶型包含1.92株菌；29種型別在不同地區(qū)交叉出現(xiàn),14種型別在不同年份交叉出現(xiàn),優(yōu)勢型別為001(5-10-4-8-24-11-33)42株,流行病學(xué)特點(diǎn)與PFGE的優(yōu)勢帶型相似；與始祖GDMT001親緣關(guān)系越近,對(duì)各種抗生素的耐藥性表現(xiàn)越相似,其中001-007(除了004)型對(duì)環(huán)丙沙星之外的其他抗生素耐藥率較高,MLVA型別與耐藥譜型AMP+CHL+NAL+SMX+STR+TCY+TMP+GEN完全一致的菌株比較多,尤其是在含單株的型別中比較常見。 5、MLVA與PFGE型能力比較顯示,MLVA分型的D值(0.9665)大于PFGE-Xba Ⅰ單酶切分型的D值(0.9286),而與PFGE-Xba Ⅰ-Bln Ⅰ雙酶切分辨能力(D值為0.9891)相當(dāng)；6株001型、4株002型、2株003型、3株005型及2株007型均對(duì)應(yīng)于PFGE-Bln Ⅰ的部分JPXA26.GD0001型及分別對(duì)應(yīng)于部分JPXA26. GD0006、0005、0036、0050、0017型,即MLVA能將Bln I酶切后的條帶進(jìn)一步區(qū)分為不同的型別。 研究結(jié)論 1、鼠傷寒為廣東省沙門菌的的優(yōu)勢血清型,2010年的構(gòu)成比最大,集中分布于廣州、中山等珠三角城市群,每年的5月、9月為發(fā)病高峰,以0-1歲的男嬰患者為主；所有鼠傷寒沙門菌對(duì)頭孢類抗生素敏感性高,對(duì)環(huán)丙沙星耐藥率最低,對(duì)其余8種抗生素高度耐藥,多重耐藥形式嚴(yán)峻,AMP+CHL+NAL+SMX+STR+TCY+TMP+GEN為最主要的多重耐藥譜型。 2、初步建立了廣東省ESS的分子分型數(shù)據(jù)庫,PFGE和MLVA分子分型方法均顯示廣東省鼠傷寒沙門菌具有遺傳多樣性。型別的聚集和分散符合菌株的總體流行病學(xué)特征。 3、兩種分型方法的DNA指紋圖譜和耐藥譜無明顯一致性。 4、MLVA的分辨能力高,不僅從宏觀上對(duì)菌株的相似性進(jìn)行分析,而且從微觀上揭示細(xì)菌的親緣進(jìn)化關(guān)系,在確認(rèn)鼠傷寒沙門菌引起的暴發(fā)事件時(shí),采用MLVA分型方法可滿足菌株聚集性分析。
[Abstract]:Research background
Foodborne disease is a kind of infectious or toxic diseases caused by various pathogenic factors feeding into the human body, every year around the world only diarrhea caused the death of millions of children, even in developed countries and at least 1/3 people suffering from foodborne disease, Salmonella (Salmonella) is one of the main pathogens of production. With the change of lifestyle, regional personnel, food contact, material circulation is more extensive, food processing, food supply and diversification of the year all over the world including China have reported foodborne disease caused by Salmonella, public health problems caused by the economic burden of health can not be ignored, gradually attracted the attention of relevant departments. There are more than 2500 serotypes of Salmonella among them, typhimurium (Salmonella enterica serovar Typhimurium, S. Typhimurium) is caused by human gastrointestinal inflammation or food poisoning The most common, one of the most important serotypes, parasitic in humans and reptiles of the animal intestinal tract is an important zoonotic pathogens. The main clinical manifestations of gastroenteritis, septicemia and other diseases caused by the bacteria Salmonella, foodborne disease outbreaks often occur at home and abroad, and cause significant economic losses.
Guangdong Province, every year there are reports of poisoning caused by Salmonella typhimurium food diarrhea outbreak concentrated outbreak by point source of the previous, more and more into the cross region "scattered in the outbreak, the spread of pollution sources and found more and more dependent on aid and confirmation on the molecular typing methods at present. The international standard has been relatively mature of pulsed field gel electrophoresis (Pulse-Field Gel Electrophoresis, PFGE) classification scheme, the second generation of molecular typing techniques of multilocus variable number tandem repeat analysis (Multiple-locus Variable-numbers Tandem Repeat Analysis, MLVA) and multilocus 7 housekeeping genes of British University of Warwick published sequence analysis (Multiple Locus Sequence Typing, MLST) typing method, investigation and successful application of food borne diseases in the outbreak caused by Salmonella typhimurium . in order to establish active monitoring system based on laboratory, improve the molecular typing of network library, Guangdong province since 2009 officially launched to strengthen the monitoring of Salmonella (Enhance Salmonella Surveillance, ESS), the monitoring of patients with sporadic diarrhea, by monitoring found Salmonella typhimurium as the dominant serotype of Salmonella typhi in non infectious diarrhea cases in the province.
The purpose of this study is to Guangdong to carry out Salmonella typhimurium molecular field monitoring, Salmonella typhimurium isolates of 2009-2011 ESS were PFGE and MLVA genotyping, DNA fingerprint based data to obtain the strains, and the analysis of monitoring points of the province hospital diarrhea Genetics Association Lin bed separation between strains, understand the molecular genetic characteristics, to find the outbreak of Salmonella typhimurium may cause the bottom incidence level, for laboratory monitoring, provide technical support and data reference identification investigation and prevention and early warning, provide decision basis for scientific prevention and control of Salmonella typhimurium caused by foodborne diseases, improve food borne disease detection and prevention and control ability.
research objective
1, we need to know the current status of surveillance system for Salmonella in Guangdong Province in the past 2009-2011 years. We mainly analyzed the epidemiological characteristics of the dominant serotype typhus, the sensitivity of antibiotics and the characteristics of multidrug resistance.
2, three years of surveillance and collection of Salmonella typhimurium were carried out for PFGE typing. The accumulation and distribution patterns of strains were analyzed to find out the epidemiological rule of banding pattern, and provide a basic molecular typing database for monitoring and outbreaks.
3, Salmonella typhimurium MLVA reference published by the PulseNet classification scheme, were optimized, and applied to strengthen monitoring system analysis, phylogenetic relationships and distribution of type MLVA were analyzed and compared with PFGE typing, typing MLVA comprehensive evaluation, value and the significance, clarify the practical application of the method is easy to realize fast typing techniques to improve laboratory active monitoring ability.
research method
1, the antibiotic susceptibility tests in Guangdong Province during 2009-2011 ESS in Salmonella typhimurium were recommended by WHO modified K-B Bauer method. According to the 2012 edition of the CLSI paper choose 12 antibiotics and interpretation sensitivity results, and finally the use of WHONET5.4 for antibiotic susceptibility test data analysis. Strains of epidemiological data and detection and input Excel2003. Numerical import SPSS13.0 software for statistical analysis, to strengthen the 2009-2011 monitoring system of the positive rate of Salmonella serovar typhimurium, advantage area, time and population distribution characteristics, descriptive statistical analysis and characteristics of antibiotic sensitivity and multidrug resistance.
2, Salmonella PFGE molecular typing according to the international PulseNet standard for the operation of the program, the advantages of Salmonella serotype Typhimurium was used for molecular typing of strains. After restriction endonuclease digestion, pulsed field gel electrophoresis, analysis system generates image strips using gel imaging, BioNumerics software for image processing, strip recognition. H9812 strain as standard molecular weight calibration. Select the unweighted pair group method with arithmetic mean of band clustering, according to the results of the analysis using the unified naming rules are numbered on the strip, the establishment of Salmonella PFGE molecular typing to strengthen monitoring database. Combined with the epidemiological data of strains, using descriptive analysis with the accumulation and distribution is the main distribution, three; chi square test for the three distribution differences and belt type of advantage, combined with the type of drug resistance spectrum correlation points Analysis.
3, 7 loci MLVA PulseNet recommended reference standard operation plan, optimize the reaction system and inspection procedures, and applied to strengthen the type monitoring system of Salmonella typhimurium analysis. Detection of PCR products amplified using ABI3730XL sequencing, the fragment size of capillary fluorescence signal corresponding to the GeneMapper software according to the common recognition, the results of agarose gel electrophoresis, the fragment size for confirmation, repeat number calculation sites, BioNumerics software for the repeat number of minimum spanning tree are analyzed, according to the analysis results using the established naming rules are numbered for MLVA type, establish Salmonella typing enhanced MLVA molecular monitoring database. Combined with the epidemiological data of strains, genetic the evolutionary relationship of the type of MLVA, analyze the distribution regularity and the relationship with the resistance spectrum, and PFGE classification were compared in To evaluate the classification ability and practical application value of MLVA.
Research results
1, three years of monitoring collection total sample number is 19895, the average annual Salmonella detection rate was 4.03% (802/19895), the average detection rate of Salmonella typhimurium, accounted for 1.38% (275/19895), 34.29% (275/802), the dominant serotype Typhimurium.275 Guangdong Salmonella strains of Salmonella typhimurium, to in 2010 the largest proportion (136/275,44.88%), every May, September is the peak incidence, male to female ratio was 1.57:1, there was no significant difference between the sexes in different years (P0.05), 0-1 years old children as the main disease groups (157/275,57.09%), there was statistical significance each year is different in different ages (P0.05), mainly from Guangzhou (95/275,34.55%), Zhongshan (59/275,21.45%) and the Pearl River Delta city group.
2275 strains of Salmonella typhimurium antibiotic sensitivity test results show that the sensitivity of the cephalosporin antibiotic, was more than 80%, while the lowest rate of ciprofloxacin (6.2%), the other 8 kinds of antibiotics were highly resistant, resistant rate was 68.4%-90.5%; in addition to ciprofloxacin, streptomycin, antibiotic resistance rate of each year is consistent in different years the strains to ampicillin, nalidixic acid, sulfamethoxazole and tetracycline resistance rate differences were statistically significant (P0.05); 3 strains of Salmonella typhimurium (ESS221.463.797) to 12 antibiotics detected all 255 strains of Salmonella typhimurium tolerance, multi drug resistance strains (92.7%), AMP+CHL+NAL+SMX+STR+TCY+TMP+GEN is Guangdong's leading multi drug resistance province profile (104/275,37.8%).
3275 strains of Salmonella typhimurium by Xba I enzyme digestion electrophoresis after the similarity value is 56.09%-100%, divided into 124 types, 7 types of clusters, the D value is 0.9286, each type contains the 1-71 strain, the average of each zone type 2.22 isolates with type.30 in different areas of cross 17, belt type appeared in different years. The advantages of cross type JPXX01.GD0004 with a total of 71 strains, including 21 strains from Guangzhou (29.6%), Zhongshan (23.9%), 17 strains of Dongguan 14 strains (19.7%), mainly in 2010 (51/71,71.8%), May (14/71,19.72%), September (11/71,15.49%) to the peak in 0-1 years old baby boy, the distribution of dominant bands consistent with the epidemiological characteristics of strains in different regions, age, gender and age distribution was statistically significant between group differences (P0.05); the predominant type of antibiotic resistant 5-9 resistant, with type JPXX01.GD0035 and JPXX01.GD0047 spectrum for AMP+CHL+NAL+SMX+STR+TCY+TMP+GEN.
MLVA of the 4275 strains of Salmonella typhimurium typing results showed that all strains were divided into 143 types, 5 phylogenetic groups, the D value is 0.9665, each type of 1-42 strain, the average of each zone type contains 1.92 strains; 29 types of cross in different regions, 14 types in different years cross, the dominant type 001 (5-10-4-8-24-11-33) of 42 strains, the epidemiological characteristics and the advantages of PFGE and similar bands; GDMT001 closer genetic relationship between ancestor, resistant to various antibiotics is similar to the 001-007 (except 004) type of ciprofloxacin outside of other antibiotic resistant rate of type MLVA and type of drug resistance spectrum AMP+CHL+NAL+SMX+STR+TCY+TMP+GEN were identical more, especially in water plant types.
5, compared to MLVA and PFGE type, MLVA type D (0.9665) than PFGE-Xba I single RFLP D value (0.9286), and resolution of PFGE-Xba I and -Bln I double enzyme (D = 0.9891); 6 strains of type 001, type 002 and 4 strains, 2 strains type 003, 3 strains of type 005 and 2 strains of type 007 are corresponding to the PFGE-Bln I JPXA26.GD0001 type and corresponding to the part of the JPXA26. GD00060005003600500017, MLVA Bln can be digested with restriction enzyme I band is further divided into different types.
research conclusion
1, Guangdong Province, Salmonella typhimurium serotypes, 2010 which is the largest, located in Guangzhou, Zhongshan and other Pearl River Delta city group, every May, September is the peak incidence, 0-1 years old male patients; all Salmonella typhimurium to cephalosporin antibiotic sensitivity to ciprofloxacin the lowest rate of other 8 kinds of antibiotics were highly resistant, multi drug resistant forms of severe, AMP+CHL+NAL+SMX+STR+TCY+TMP+GEN is the main type of multi drug resistant spectrum.
2, the molecular typing database of ESS in Guangdong province was preliminarily established. The typing methods of PFGE and MLVA showed that the Salmonella typhimurium in Guangdong province had genetic diversity. The aggregation and dispersion of type were in line with the general epidemiological characteristics of the strain.
3, there was no obvious consistency between the DNA fingerprints and the resistance spectrum of the two types of typing.
4, MLVA has high resolving power. It not only analyzes the similarity of bacteria from macroscopic view, but also reveals the phylogenetic relationship of bacteria from microcosmic aspect. When confirming the outbreak caused by Salmonella typhimurium, MLVA typing method can be applied to analyze the aggregation of bacteria.

【學(xué)位授予單位】：南方醫(yī)科大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2014
【分類號(hào)】：R516.3

【參考文獻(xiàn)】

相關(guān)期刊論文 前10條

1 李劍森;梁駿華;柯碧霞;盧玲玲;何冬梅;鄧小玲;柯昌文;黃蔚;黃熙;李世聰;黃瓊;;2012年廣東省食源性疾病監(jiān)測結(jié)果分析[J];華南預(yù)防醫(yī)學(xué);2013年06期

2 石曉路;劉小立;黃薇;王冰;林一曼;賀連華;吳平芳;扈慶華;;脈沖場凝膠電泳在沙門菌食物中毒溯源中的應(yīng)用研究[J];疾病控制雜志;2007年05期

3 譚瑤;趙清;舒為群;陳浩;;K-B紙片擴(kuò)散法藥敏試驗(yàn)[J];檢驗(yàn)醫(yī)學(xué)與臨床;2010年20期

4 張京云;聶艷妮;陳春霞;刁保衛(wèi);婁靜;闞飆;閆梅英;;脈沖場凝膠電泳和多位點(diǎn)串聯(lián)重復(fù)序列分析應(yīng)用于中國鼠傷寒沙門菌分型能力的評(píng)價(jià)[J];疾病監(jiān)測;2011年04期

5 周世明,賈杰;耐藥性傷寒的抗菌治療新進(jìn)展[J];中國熱帶醫(yī)學(xué);2003年04期

6 許學(xué)斌;金匯明;肖文佳;陳敏;冉陸;刁保衛(wèi);崔志剛;闞飚;;上海市鼠傷寒沙門菌流行特征及分子分型研究[J];上海預(yù)防醫(yī)學(xué);2010年02期

7 邱亞群;林一曼;扈慶華;石曉路;李迎慧;劉濤;;深圳市119株沙門菌的菌型分布及耐藥情況分析[J];實(shí)用預(yù)防醫(yī)學(xué);2010年08期

8 王茂起,冉陸,王竹天,李志剛,姚景惠,付萍,楊寶蘭,徐進(jìn),李迎惠;2001年中國食源性致病菌及其耐藥性主動(dòng)監(jiān)測研究[J];衛(wèi)生研究;2004年01期

9 趙晉;楊小蓉;徐耀方;何樹森;;2007年四川省鼠傷寒沙門菌PFGE分型及溯源[J];預(yù)防醫(yī)學(xué)情報(bào)雜志;2009年12期

10 孔繁林;儲(chǔ)從家;;甲型副傷寒沙門菌的耐藥現(xiàn)狀與臨床用藥[J];中國微生態(tài)學(xué)雜志;2007年01期

，

本文編號(hào)：1439749