本文關鍵詞：微孔板稀釋法檢測結核分枝桿菌丙硫異煙胺耐藥結果分析　出處：《中國人獸共患病學報》2017年04期 　論文類型：期刊論文

  更多相關文章： 結核分枝桿菌 丙硫異煙胺 藥物敏感試驗

【摘要】：目的以MGIT960快速藥敏法為參照,微孔板稀釋法檢測結核分枝桿菌丙硫異煙胺耐藥的效果。方法采用MGIT960快速藥敏(MGIT法)和微孔板稀釋法對248株結核分枝桿菌臨床分離株進行丙硫異煙胺藥敏試驗,檢測結核分枝桿菌對該藥物的耐藥性。隨機選取117株菌株進行該藥物的11個濃度梯度(0.0625、0.125、0.25、0.5、1、2、4、8、16、32、64μg/mL)的藥敏檢測,比較兩種方法最低抑菌濃度的一致性。結果 MGIT法的平均檢測時間為10.1d,微孔板稀釋法檢測時間為8d,兩者差異有統(tǒng)計學意義(P0.001)。與MGIT法測定結果比較,微孔板稀釋法檢測的敏感度為96.5%(55/57),特異度為93.2%(178/191),符合率為94.0%(233/248),Kappa值為0.84。兩種方法所獲得的MIC值的總一致率為81.2%(95/117),其中在MIC值8.0μg/mL的分離株中,兩種方法的一致率為86.3%(82/95);在MIC值≥8.0μg/mL的分離株中,兩種方法的一致率僅為59.1%(13/22)。結論兩種方法對丙硫異煙胺進行耐藥檢測有較高的一致性,但也存在一定的差異。微孔板稀釋法比MGIT法簡單,快速,適合用于結核分枝桿菌對丙硫異煙胺耐藥性的快速檢測。
[Abstract]:Objective to use MGIT960 rapid drug sensitivity assay as a reference. Effect of micropore plate dilution method on the detection of mycobacterium tuberculosis resistance to propanolicotinamide. Methods MGIT960 rapid drug sensitive method was used to detect the resistance of Mycobacterium tuberculosis to isonicotinamide. And micropore plate dilution method was used to detect the drug sensitivity of 248 clinical isolates of Mycobacterium tuberculosis. The drug resistance of Mycobacterium tuberculosis to the drug was tested. 117 strains were randomly selected to carry out the drug's 11 concentration gradient of 0.0625 0.125 ~ 0.25 ~ (0.5) ~ (1) ~ (2) ~ (2) ~ (4). The drug sensitivity of the two methods was compared with that of the two methods. Results the average detection time of the MGIT method was 10.1 days. The detection time of microporous plate dilution method was 8 days, the difference was statistically significant (P 0.001). The results were compared with MGIT method. The sensitivity of microporous plate dilution method was 96.555 / 57, the specificity was 93.2and 17.8 / 191a, the coincidence rate was 94.0 / 243 / 248). The Kappa value was 0.84.The total consistent rate of the MIC value obtained by the two methods was 81.2% 117g / mL, and the MIC value was 8.0 渭 g / mL. The concordance rate of the two methods was 86.3 / 95. In the isolates with MIC 鈮，

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