本文關(guān)鍵詞：北京市2007-2014年O1群霍亂弧菌病原學(xué)特征分析　出處：《中國(guó)預(yù)防醫(yī)學(xué)雜志》2016年10期 　論文類型：期刊論文

  更多相關(guān)文章： 霍亂弧菌 藥物敏感試驗(yàn) 毒力基因 脈沖場(chǎng)凝膠電泳

【摘要】：目的分析北京市2007-2014年分離到的O1群霍亂弧菌的病原學(xué)特征。方法對(duì)霍亂感染者、水產(chǎn)品和外環(huán)境來源的O1群霍亂弧菌,采用血清分型、熒光定量PCR檢測(cè)霍亂毒素基因ctxAB、K-B法8種藥物(多西環(huán)素、環(huán)丙沙星、諾氟沙星、復(fù)方新諾明、丁胺卡那霉素、慶大霉素、頭孢曲松和呋喃妥因)進(jìn)行敏感性試驗(yàn)和脈沖場(chǎng)凝膠電泳(PFGE)分子分型方法,研究不同來源菌株在各方面的異同。結(jié)果北京市2007-2014年共分離得到不同來源O1群霍亂弧菌125株(感染者75株,水產(chǎn)品37株,外環(huán)境涂抹13株),小川型96株,稻葉型29株。其中16株菌ctxAB基因陽性(感染者5株,水產(chǎn)品9株,外環(huán)境涂抹2株),其他ctxAB基因陰性(感染者70株,水產(chǎn)品28株,外環(huán)境涂抹11株);小川型ctxAB基因陰性霍亂弧菌占70.40%(88/125);125株菌對(duì)慶大霉素、頭孢曲松和呋喃妥因完全敏感,其中25株菌(感染者10株,水產(chǎn)品11株,外環(huán)境4株)對(duì)5種抗菌藥物出現(xiàn)7種耐藥型別,有6株來自感染者和水產(chǎn)品的菌株產(chǎn)生雙重或三重耐藥,而4株外環(huán)境的耐藥菌株均為單一耐藥。PFGE分子分型結(jié)果顯示,125株菌株經(jīng)NotⅠ酶切后可分為91種型別,7起聚集性疫情中分離到的37株菌全部為小川型,可分為6個(gè)聚類,29株稻葉型菌株可分為3個(gè)聚類。結(jié)論北京市2007-2014年O1群霍亂弧菌病原學(xué)特征復(fù)雜多樣,在產(chǎn)毒株引起霍亂弧菌疫情減少的情況下,應(yīng)關(guān)注非產(chǎn)毒霍亂弧菌導(dǎo)致的疫情。
[Abstract]:Objective to analyze the pathogenic characteristics of Vibrio cholerae (Vibrio cholerae) isolated from the O1 group of Beijing in 2007-2014 years. The method of cholera infection, sources of environmental products and external water of Vibrio cholerae O1 serotype, using fluorescence quantitative PCR detection of cholera toxin gene ctxAB and K-B method of 8 kinds of drugs (doxycycline, norfloxacin, ciprofloxacin, cotrimoxazole, amikacin, gentamicin, ceftriaxone and nitrofurantoin) sensitivity test and pulsed field gel electrophoresis (PFGE) molecular typing method, to study the similarities and differences of different strains in all aspects. The results of 2007-2014 in Beijing city were isolated from different sources of Vibrio cholerae O1 125 strains (75 strains of infection, aquatic products of 37 strains, 13 strains with the external environment), 96 Ogawa strains, 29 strains of rice leaf. The ctxAB gene from 16 strains were positive (infected with 5 strains, 9 strains of aquatic products, the external environment with 2 strain), other ctxAB gene negative (infected 70 strains, 28 strains of aquatic products, the external environment with 11 strains); ctxAB gene negative Vibrio cholerae Ogawa accounted for 70.40% (88/125); 125 strains of micin, ceftriaxone and nitrofurantoin completely sensitive to the 25 strains (10 strains of infection, aquatic products of 11 strains, 4 strains of the external environment) to 5 kinds of antibiotics resistant type 7, 6 strains from infected and aquatic products isolates produced double or three heavy resistance, and the resistance of 4 strains were resistant to a single environment. PFGE molecular typing results showed that 125 strains were digested by Not I can be divided into 91 types, separated by 7 aggregation of the outbreak of the 37 strains all Ogawa, can be divided into 6 clusters, 29 strains of Inaba strains can be divided into 3 clusters. Conclusion the pathogenic characteristics of Vibrio cholerae of group O1 in Beijing city are complicated in the past 2007-2014 years. We should pay attention to the epidemic situation caused by Vibrio cholerae.
【作者單位】： 北京市疾病預(yù)防控制中心;北京市朝陽區(qū)疾病預(yù)防控制中心;中國(guó)疾病預(yù)防控制中心;
【基金】：國(guó)家科技重大專項(xiàng)項(xiàng)目(2012ZX10004215-003-001) 北京市衛(wèi)生系統(tǒng)高層次衛(wèi)生技術(shù)人才培養(yǎng)計(jì)劃(2013-3-099)
【分類號(hào)】：R516.5
【正文快照】： 霍亂是由O1血清群和O139血清群霍亂弧菌引起的急性腸道傳染病,是我國(guó)傳染病防治法規(guī)定管理的甲類傳染病�；魜y弧菌感染者(包括病例和帶菌者)是霍亂的傳染源,霍亂經(jīng)糞-口途徑傳播,主要傳播方式為攝入污染的食物或水、通過生活接觸等。O1群霍亂弧菌根據(jù)流行階段病原的特征,分為

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