【摘要】：目的：1．觀察地塞米松對(duì)紅色毛癬菌生長(zhǎng)、菌落產(chǎn)色及抗真菌藥物敏感性的影響，為臨床及時(shí)準(zhǔn)確的診斷和合理的用藥提供理論依據(jù)。2．探討紅色毛癬菌在地塞米松干預(yù)前后基因型的差異，從基因水平探討地塞米松影響下紅色毛癬菌表型特征發(fā)生變化的可能分子生物學(xué)機(jī)制。 方法：1．對(duì)臨床上經(jīng)分離鑒定的紅色毛癬菌首先采用大培養(yǎng)測(cè)定含不同濃度地塞米松沙氏培養(yǎng)基上菌落生長(zhǎng)直徑，并繪制生長(zhǎng)曲線，然后采用分光光度計(jì)測(cè)定含不同濃度地塞米松沙氏液體培養(yǎng)基的吸光度，最后參照美國(guó)國(guó)家實(shí)驗(yàn)室標(biāo)準(zhǔn)委員會(huì)(NCCLS M-38A)方案，采用微量液基法檢測(cè)不同濃度地塞米松干預(yù)下特比萘芬對(duì)紅色毛癬菌的MIC變化。2．取在含不同濃度地塞米松培養(yǎng)基上生長(zhǎng)的紅色毛癬菌，采用真菌通用引物ITS1、ITS4對(duì)其ITS區(qū)進(jìn)行PCR擴(kuò)增，從分子水平對(duì)其進(jìn)行鑒定，經(jīng)鑒定的紅色毛癬菌進(jìn)一步對(duì)其NTS區(qū)進(jìn)行PCR擴(kuò)增，觀察不同濃度地塞米松干預(yù)前后基因條帶的變化。 結(jié)果：1．從繪制的生長(zhǎng)曲線看，當(dāng)?shù)厝姿傻臐舛葹?.05％、0.1％和0.2％時(shí)，與對(duì)照組比較無(wú)明顯的變化，當(dāng)?shù)厝姿傻臐舛葹?.4％、0.8％時(shí)前五天對(duì)紅色毛癬菌生長(zhǎng)有明顯的抑制作用，其后促進(jìn)紅色毛癬菌的生長(zhǎng)，當(dāng)?shù)厝姿傻臐舛葹?.0％時(shí)對(duì)紅色毛癬菌生長(zhǎng)有明顯的抑制作用。2．不含地塞米松組紅色毛癬菌的吸光度值(OD)幾何均數(shù)為0.117±0.032，特比萘芬MIC為0.063μg／ml，當(dāng)?shù)厝姿傻臐舛葹?.05％、0.1％和0.2％時(shí)，，紅色毛癬菌的OD幾何均數(shù)分別為0.118±0.032、0.118±0.032和0.132±0.030，MIC分別為0.081μg／ml、0.092μg／ml和0.109μg／ml與空白組比較無(wú)明顯差異(P＞0.05)；當(dāng)其濃度為0.4％、0.8％時(shí)，紅色毛癬菌的OD幾何均數(shù)分別為0.165±0.081、0.175±0.085，特比萘芬MIC分別為0.196μg／ml、0.319μg／ml與空白組比較有明顯的差異(P＜0.05)。3．引物ITS1、ITS4在不同濃度地塞米松沙氏培養(yǎng)基上生長(zhǎng)的紅色毛癬菌均擴(kuò)增出一約690bp特異條帶，5株紅色毛癬菌用引物TrNTSF-2和
[Abstract]:Objective: 1. To observe the effect of dexamethasone on the growth, colony color production and antifungal drug sensitivity of Trichophyton rubrum. To provide theoretical basis for timely and accurate diagnosis and reasonable drug use in clinic. 2. To explore the genotypic difference of Trichophyton rubrum before and after dexamethasone intervention. To explore the possible molecular biological mechanism of phenotypic changes of Trichophyton rubrum under the influence of dexamethasone at gene level. Methods: 1. The colony growth diameter of Trichophyton rubrum isolated and identified in clinic was determined by large culture, and the growth curve was drawn. Then the absorbance of the liquid medium containing different concentrations of dexamethasone was determined by spectrophotometer. Finally, the National Laboratory Standards Committee (NCCLS M 鈮

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