【摘要】：目的分析江西省2009-2012年甲型H3N2流感病毒M2以及NA基因的特點(diǎn),掌握其耐藥情況,為臨床治療和疾病控制提供參考。方法從江西省流感監(jiān)測(cè)網(wǎng)中隨機(jī)選擇19株甲型H3N2流感病毒,經(jīng)核酸提取和one-step RT-PCR擴(kuò)增M以及NA基因片段,雙向序列測(cè)定,采用DNAStar5.0和Mage4.0序列分析軟件分析M2以及NA基因特征以及耐藥性位點(diǎn)。結(jié)果 19株毒株M2基因31位氨基酸均由絲氨酸(S)突變?yōu)樘於０?N);所有毒株NA蛋白催化活性位點(diǎn)和輔助位點(diǎn)均未發(fā)生氨基酸替換。結(jié)論 19株毒株均對(duì)金剛烷胺類(lèi)藥物耐藥,對(duì)流感病毒神經(jīng)氨酸酶抑制劑藥物均敏感,但仍應(yīng)加強(qiáng)對(duì)流感病毒的耐藥性監(jiān)測(cè)。
[Abstract]:Objective to analyze the characteristics of M 2 and NA genes of influenza A virus (H3N2) in Jiangxi Province from 2009 to 2012, and to provide reference for clinical treatment and disease control. Methods 19 strains of influenza A virus (H3N2) were randomly selected from Jiangxi Influenza Surveillance Network. M and NA gene fragments were amplified by nucleic acid extraction and one-step RT-PCR and sequenced in two directions. The characteristics and drug resistance sites of M2 and NA genes were analyzed by DNAStar5.0 and Mage4.0 sequence analysis software. Results the amino acid position 31 of M2 gene of 19 strains was mutated from serine (S) to asparagine (N);. There were no amino acid substitutions in NA protein catalytic active sites and auxiliary sites of all strains. Conclusion all 19 strains are resistant to amantadine and sensitive to influenza virus neuraminidase inhibitors, but the surveillance of resistance to influenza virus should be strengthened.
【作者單位】： 江西省疾病預(yù)防控制中心;
【基金】：江西省衛(wèi)生廳科技項(xiàng)目(編號(hào):20112003)
【分類(lèi)號(hào)】：R373;R3416

【參考文獻(xiàn)】

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1 盧桂蘭;彭曉e，

本文編號(hào)：2464416