【摘要】： 目的建立腺嘌呤誘發(fā)的大鼠慢性腎衰模型，探討腎間質(zhì)纖維化的發(fā)生與a-平滑肌肌動(dòng)蛋白(a-SMA)表達(dá)的關(guān)系。 方法20只SD大鼠隨機(jī)分為正常對(duì)照組和腎衰模型組，正常組以生理鹽水10ml／kg／d灌胃；模型組以腺嘌呤250mg／kg／灌胃，共24天(第1～12天每日給藥1次，第12天后隔日給藥)。檢測(cè)血清尿素氮、肌酐，，Ca和P含量，觀察腎組織的病理改變和纖維的增生程度，免疫組化采用SP法觀察腎組織中a-SMA和Ⅳ型膠原的表達(dá)。 結(jié)果與正常組比較模型組大鼠血清尿素氮和肌酐含量明顯升高(p＜0.05)，Ca、P代謝失去平衡，腎小球數(shù)量明顯減少，腎間質(zhì)內(nèi)大量纖維組織增生，大量a-SMA陽(yáng)性細(xì)胞和Ⅳ型膠原在腎間質(zhì)中分布。 結(jié)論腺嘌呤誘發(fā)的大鼠慢性腎衰模型是研究慢性腎衰和腎間質(zhì)纖維化的較理想的動(dòng)物模型，a-SMA陽(yáng)性細(xì)胞在腎間質(zhì)纖維化的發(fā)生過(guò)程中發(fā)揮重要的作用。
[Abstract]:Objective to establish a rat model of chronic renal failure induced by adenine and to investigate the relationship between the pathogenesis of renal interstitial fibrosis and the expression of a- smooth muscle actin (a-SMA). Methods Twenty SD rats were randomly divided into two groups: normal control group and renal failure model group. Normal group was given normal saline 10ml/kg/d. In the model group, adenine 250mg/kg/ was given orally for 24 days (once a day on the 12th day and every other day after the 12th day). The contents of serum urea nitrogen, creatinine, Ca and P, the pathological changes of renal tissue and the degree of fibrous hyperplasia were observed. The expression of a-SMA and type 鈪

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