【摘要】：骨折延遲愈合、不愈合、骨缺損與骨質(zhì)疏松癥臨床治療效果不理想。近年來，，現(xiàn)代骨組織工程學(xué)迅速發(fā)展，為解決這些難題提供了可能。組織種子細(xì)胞的獲得、培養(yǎng)與分化是此工程的基礎(chǔ)。研究者尋找到具有強(qiáng)大的體外增殖力和多向系分化潛能的人骨髓間充質(zhì)干細(xì)胞(human bone marrow mesenchymal stem cells，hBMSCs)。由于hBMSCs可在體外培養(yǎng)并能在植入體內(nèi)后成骨，故骨組織工程以hBMSCs作為種子細(xì)胞應(yīng)用于臨床已成為趨勢(shì)。將誘導(dǎo)成骨因素應(yīng)用于骨組織工程，是近年來該工程研究的一個(gè)主要課題。自古以來，骨碎補(bǔ)就作為一種骨傷科的良藥應(yīng)用于臨床�，F(xiàn)代研究證實(shí)骨碎補(bǔ)能促進(jìn)成骨，但機(jī)制不清楚。骨碎補(bǔ)種類有12種以上，制劑工藝多樣，各種成品活性成分各異。研究發(fā)現(xiàn)，柚皮甙是骨碎補(bǔ)的主要活性成分，本課題以柚皮甙為研究對(duì)象，探討其誘導(dǎo)hBMSCs分化為成骨細(xì)胞與骨細(xì)胞的影響和機(jī)制。 第一章 人骨髓間充質(zhì)干細(xì)胞的分離、體外培養(yǎng)及生物學(xué)特性的觀察 目的 分離、培養(yǎng)hBMSCs，觀察傳代與年齡對(duì)hBMSCs生物學(xué)特性的影響。 方法 利用公認(rèn)的hBMSCs分離方法密度梯度離心法配合貼壁篩選法、細(xì)胞形態(tài)學(xué)觀察、免疫細(xì)胞化學(xué)染色、流式細(xì)胞術(shù)檢測(cè)細(xì)胞表面標(biāo)志和后期誘導(dǎo)hBMSCs分化結(jié)果等方法鑒定所分離、培養(yǎng)的為hBMSCs。按供體年齡分為A、B兩組。用倒置相差顯微鏡、透射電鏡觀察細(xì)胞的一般形態(tài)、
[Abstract]:Delayed union, non-union, bone defect and osteoporosis are not effective. In recent years, the rapid development of modern bone tissue engineering provides the possibility to solve these problems. The acquisition, culture and differentiation of tissue seed cells are the basis of this project. The researchers looked for human bone marrow mesenchymal stem cells (human bone marrow mesenchymal stem cells,hBMSCs) with strong proliferative capacity and multilineage differentiation potential in vitro. Because hBMSCs can be cultured in vitro and osteogenic after implantation, it has become a trend to use hBMSCs as seed cells in bone tissue engineering. The application of osteogenic factors in bone tissue engineering is a major research topic in recent years. Since ancient times, broken bone has been used as a good medicine in orthopedics. Modern studies have shown that Oscillation can promote osteogenesis, but the mechanism is not clear. There are more than 12 kinds of pellet, the preparation process is various, and the active components of each kind of finished product are different. It was found that naringin is the main active component of osteoclastic material. In this study, naringin was used as the research object to investigate the effect and mechanism of naringin on the differentiation of hBMSCs into osteoblasts and osteoblasts. Chapter 1 isolation of human bone marrow mesenchymal stem cells in vitro culture and observation of biological characteristics objective to isolate the effects of passage and age of cultured hBMSCs, on the biological characteristics of hBMSCs. Methods the method of density gradient centrifugation, adherent screening, cell morphology observation, immunocytochemical staining, flow cytometry and late induction of hBMSCs differentiation were used to identify the hBMSCs. Cultured for hBMSCs. Group A was divided into two groups according to donor age. The general morphology of cells was observed by inverted phase contrast microscope and transmission electron microscope.
【學(xué)位授予單位】：中南大學(xué)
【學(xué)位級(jí)別】：博士
【學(xué)位授予年份】：2006
【分類號(hào)】：R329.2

【引證文獻(xiàn)】

相關(guān)期刊論文 前1條

1 郭延偉;李松;房殿吉;;杜仲水/醇提取物促進(jìn)兔脂肪基質(zhì)干細(xì)胞增殖及成骨分化[J];中國組織工程研究;2012年32期

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