【摘要】： 目的:建立肝包蟲動物模型并觀察包蟲囊壁情況,研究骨橋蛋白在小鼠肝細粒棘球蚴囊壁周圍的分布特征及表達。 方法:選擇同窩同種小鼠180只,隨機分為免疫正常組及免疫抑制組,每組各90只,一組小鼠制作非特異性免疫抑制模型。將以上免疫正常及免疫抑制組按隨機的方法又分別分為1、2、3組,每組30只,分別采用門靜脈穿刺注射、開腹直視下肝臟穿刺注射及經皮肝臟穿刺注射細粒棘球蚴(Echinococcus granulosus,E.g)原頭節(jié)混懸液的方法,制備肝E. g動物模型,免疫正常組作HE染色觀察,免疫組化觀察小鼠肝細粒棘球蚴外囊壁中骨橋蛋白(Osteopontin ,OPN)的表達與分布。 結果:免疫正常組小鼠肝臟E. g感染率依據(jù)接種方法不同分別為93.1%、80.2%及59.3% ,免疫抑制組感染率分別為93.3%、79.3%及56.4%,兩組無明顯差異(P0.05)。HE切片染色顯示外囊與周圍組織具有明確界線,不存在漸變的過程, 83%肝細粒棘球蚴外囊壁外側有不同程度OPN表達,且分布與囊壁外側炎性細胞分布一致,在外囊層僅有少量OPN表達(P0.01)。 結論:門靜脈穿刺、開腹肝穿及經皮肝穿均可制備繼發(fā)性肝臟E. g動物模型,且免疫正常組與抑制組感染率、包囊直徑及包囊個數(shù)無明顯差別,其中門靜脈穿刺注射法較其他兩種方法接種感染率高,包囊個數(shù)多,為理想的肝E. g動物模型制備方法,同時在動物模型上證實了早期肝細粒棘球蚴外囊為肉芽腫反應結果, OPN主要分布在肝細粒棘球蚴壁囊外側炎性細胞中,并在外囊的形成過程中起重要作用。
[Abstract]:Aim: to establish an animal model of hepatic hydatid and to study the distribution and expression of osteopontin around the cyst wall of hepatic hydatid in mice. Methods: 180 homologous mice of the same nest were randomly divided into normal immune group and immunosuppressive group with 90 mice in each group. A non-specific immunosuppressive model was made in one group of mice. The above immune normal and immunosuppressive groups were randomly divided into 3 groups with 30 rats in each group. Each group was injected with portal vein puncture, liver puncture under open vision and percutaneous hepatic injection of (Echinococcus granulosus, (Echinococcus granulosus). E.G) the animal model of liver Eg was established by the suspension of proto-cephalic ganglion. The expression and distribution of osteopontin (Osteopontin, OPN) in the wall of hepatic hydatid of mice were observed by HE staining and immunohistochemistry. Results: according to the different inoculation methods, the liver Eg infection rate of the immunized normal mice was 93.2% and 59.3%, respectively. The infection rate of the immunosuppressive group was 93.3% and 56.4%, respectively, and that of the immunosuppressive group was 79.3% and 56.4%, respectively. There was no significant difference between the two groups (P0.05). HE staining showed that there was a clear boundary between the outer capsule and the surrounding tissues, and there was no gradual change. 83% of the patients had different degree of OPN expression in the outer wall of the epicystic wall of Echinococcus granulosus. The distribution was consistent with the distribution of inflammatory cells on the lateral wall of the capsule. There was only a small amount of OPN expression in the outer capsule (P0.01). Conclusion: portal vein puncture, open liver puncture and percutaneous liver puncture can make the animal model of secondary liver Eg, and there is no significant difference in infection rate, cyst diameter and number of cyst between immune normal group and inhibition group. Among them, the portal vein puncture injection method has higher infection rate and more cyst than the other two methods. It is an ideal method for the preparation of liver Eg animal model. At the same time, the results of granulomatous reaction of the early hepatic hydatid cyst were confirmed in the animal model. OPN mainly distributes in the inflammatory cells of the lateral wall of the hepatic hydatid wall and plays an important role in the formation of the outer capsule.
【學位授予單位】：石河子大學
【學位級別】：碩士
【學位授予年份】：2007
【分類號】：R-332

【參考文獻】

相關期刊論文 前9條

1 鄭宏,徐志新,溫浩,尹極峰,汪師貞;綿羊感染細粒棘球蚴疾病模型的制備及評價[J];地方病通報;2000年01期

2 彭心宇,吳向未,張示杰,牛建華;肝包蟲囊腫周圍纖維囊壁病理結構的再認識[J];世界華人消化雜志;2005年03期

3 趙耘,張文寶,張壯志,哈斯巴提,齊普生;包蟲病動物模型的建立[J];中國獸醫(yī)科技;1997年09期

4 彭心宇;肝包蟲病的外科治療新觀點[J];中國實用外科雜志;2003年11期

5 謝增如,劉大鵬,溫浩,買買提江·阿布都卡得爾,阿德力·阿布都熱西提,艾合買提·玉素甫;骨細粒棘球蚴病動物模型的建立[J];中國寄生蟲病防治雜志;2005年02期

6 吳向未,彭心宇,張示杰,牛建華,孫紅,習羽;肝脾棘球蚴囊周圍纖維性囊壁形成機制的差異及臨床意義[J];中國寄生蟲學與寄生蟲病雜志;2004年01期

7 李建輝;彭心宇;周宗瑤;吳向未;張示杰;牛建華;孫紅;;骨橋蛋白在肝細粒棘球蚴外囊壁中的表達[J];中國寄生蟲學與寄生蟲病雜志;2006年03期

8 吳向未,陳雪玲,彭心宇,張示杰,牛建華,孫紅;Ⅰ、Ⅲ、Ⅳ型膠原在肝包蟲囊腫周圍人體纖維囊壁中的特異性分層表達[J];中國人獸共患病雜志;2004年10期

9 馬玲,王紅梅,閆向東,鄭珊;免疫失衡動物模型復制初探[J];中國食品衛(wèi)生雜志;2001年06期

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