【摘要】：第一部分：大鼠胚腦皮質(zhì)神經(jīng)元細(xì)胞的原代培養(yǎng)、鑒定和缺氧模型的建立 目的：進行大鼠胚腦皮質(zhì)神經(jīng)元細(xì)胞培養(yǎng)和鑒定，并建立大鼠胚腦皮質(zhì)神經(jīng)元細(xì)胞培養(yǎng)的缺氧模型。 方法：(1)運用胰蛋白酶和DNase Ⅰ聯(lián)合酶消化法，選擇合適孔徑的篩網(wǎng)過濾，，并以L-多聚賴氨酸作為神經(jīng)元體外培養(yǎng)的生長基質(zhì)，進行大鼠胚腦皮質(zhì)神經(jīng)元的體外培養(yǎng)；(2)運用免疫熒光組織化學(xué)方法檢測神經(jīng)元特異的抗TubulinβⅢ單克隆抗體和神經(jīng)膠質(zhì)細(xì)胞特異的抗GFAP多克隆抗體分別進行神經(jīng)元細(xì)胞和神經(jīng)膠質(zhì)細(xì)胞的鑒定，檢測體外培養(yǎng)大鼠胚腦皮質(zhì)神經(jīng)元細(xì)胞的純度；(3)運用國產(chǎn)厭氧培養(yǎng)箱在37℃含85％N_2、5％CO_2、10％H_2混合氣體環(huán)境下建立大鼠胚腦皮質(zhì)神經(jīng)元細(xì)胞的缺氧模型。 結(jié)果：(1)通過體外培養(yǎng)獲得了純度達(dá)95％以上的大鼠胚腦皮質(zhì)神經(jīng)元細(xì)胞；(2)運用神經(jīng)元特異的抗TubulinβⅢ單克隆抗體和神
[Abstract]:The first part: primary culture, identification and anoxic model of rat embryonic cortical neurons objective: culture and identification of rat embryonic cortical neurons, An anoxic model of cultured rat embryonic cortical neurons was established. Methods: (1) the rat embryonic cortical neurons were cultured in vitro using trypsin and DNase 鈪

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