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羥基脲制作SD大鼠腎陽虛證模型的規(guī)范化研究

發(fā)布時間:2018-11-14 11:56
【摘要】:一、研究目的 1.探索用羥基脲(Hydroxyacrbamide,Hu)制作SD大鼠腎陽虛證動物模型的具體方法,包括造模要素的選擇,造模過程的技術(shù)質(zhì)控,模型穩(wěn)定性研究、模型自然恢復(fù)期的研究,不同造模方法的比較研究等。為腎陽虛證的實驗和藥學(xué)研究提供較為標(biāo)準的中醫(yī)證候動物模型。 2.探索符合中醫(yī)學(xué)理論和經(jīng)驗的腎陽虛證大鼠模型評價體系,包括模型病因診斷、癥狀體征診斷、客觀指標(biāo)確立、治療反證等。為腎陽虛證動物模型和其他中醫(yī)證候動物模型規(guī)范化研究提供參考。 二、研究方法 1.理論研究 系統(tǒng)回顧了中醫(yī)證候動物模型的發(fā)展概況,對腎陽虛證的基礎(chǔ)理論、腎陽虛證動物模型的制作方法等方面進行了整理,對羥基脲腎陽虛證動物模型的研究現(xiàn)狀進行了總結(jié)。 2.實驗研究 2.1實驗一:篩選羥基脲腎陽虛證大鼠模型的造模方法 本實驗選擇SPF級雄性SD大鼠,用文獻記載的用藥劑量:190mg/kg、280mg/kg、375mg/kg、450mg/kg造模,分別在第7天、12天、17天、22天連續(xù)采血,做出各劑量組動物血清皮質(zhì)醇(Cort)、睪酮(T)、雌二醇(E_2)、甲狀腺素T_3和T_4、環(huán)磷酸腺苷(cAMP)、環(huán)磷酸鳥苷(cGMP)等各項指標(biāo)隨劑量、時間變化的動態(tài)曲線,與空白對照組作對照,確定用藥劑量和給藥時間,篩選出較為規(guī)范的造模方法。 2.2實驗二:羥基脲造模法對雄性SD大鼠癥狀及其它指標(biāo)的影響 按實驗一篩選的造模方法造模,在造模過程中對動物進行一般情況的觀察,并進行耐力耐寒試驗、抓取激惹試驗、跑步機試驗、曠場試驗、母鼠懷孕率等試驗,與空白對照組作對照。通過對癥狀的科學(xué)觀察,補充實驗一客觀指標(biāo)檢測的不足,并進一步完善腎陽虛證動物模型癥狀診斷評價體系。
[Abstract]:First, the purpose of the study 1. To explore the specific methods of making the animal model of kidney yang deficiency syndrome with hydroxyurea (Hydroxyacrbamide,Hu) in SD rats, including the selection of model elements, the technical quality control of model making process, the study of model stability, and the study of the natural recovery period of the model. Comparative study of different modeling methods. To provide a more standard animal model of TCM syndromes for the experimental and pharmacological study of kidney-yang deficiency syndrome. 2. To explore the evaluation system of rat model of kidney-yang deficiency syndrome in accordance with the theory and experience of traditional Chinese medicine, including model etiology diagnosis, diagnosis of symptoms and signs, establishment of objective indexes, treatment of adverse evidence, and so on. To provide a reference for the standardization of animal model of kidney yang deficiency syndrome and other animal models of TCM syndrome. Second, research method 1. The development of animal model of TCM syndromes was reviewed systematically, and the basic theory of kidney-yang deficiency syndrome and the method of making animal model of kidney-yang deficiency syndrome were sorted out. The present situation of research on the animal model of kidney yang deficiency syndrome with hydroxyurea was summarized. 2. Experimental study 2.1 experiment 1: screening the model of rat model of kidney yang deficiency syndrome with hydroxyurea. In this experiment, the male SD rats of SPF grade were selected. The dose recorded in the literature: 190mg / kg 280 mg / kg 250 mg 路kg ~ (-1) 路kg ~ (-1) 350 mg 路kg ~ (-1) 路kg ~ (-1). Blood samples were taken on the 7th, 12th, 17th and 22nd days, respectively, and the serum cortisol (Cort), testosterone (T), was made in each dose group. The dynamic curves of estradiol (E _ 2), thyroxine T _ 3 and T _ 4, cyclic adenosine (cAMP), cyclic guanosine phosphate (cGMP) with dose and time were compared with those of the blank control group. The dosage and time of administration were determined, and the more standard modeling method was screened out. 2.2 experiment 2: the effects of hydroxyurea modeling on symptoms and other indexes of male SD rats were studied according to the model making method selected in experiment 1, and the general conditions of the animals were observed during the course of modeling. Endurance cold tolerance test, grasping irritation test, treadmill test, open field test, pregnant rate of female mice were carried out, and compared with the blank control group. Through the scientific observation of the symptoms, the deficiency of the objective index detection was supplemented, and the diagnosis and evaluation system of the symptoms of the animal model of kidney yang deficiency syndrome was further improved.
【學(xué)位授予單位】:廣州中醫(yī)藥大學(xué)
【學(xué)位級別】:碩士
【學(xué)位授予年份】:2005
【分類號】:R-332

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