發(fā)布時(shí)間：2018-11-11 21:13

【摘要】：目的在晶狀體上皮細(xì)胞中通過(guò)蛋白酶抑制劑MG132抑制泛素-蛋白酶體途徑活性,探索其對(duì)自噬作用的影響及相關(guān)機(jī)制。方法使用蛋白酶抑制劑MG132作用于晶狀體上皮細(xì)胞,阻斷泛素-蛋白酶體途徑的功能,通過(guò)蛋白免疫印跡法檢測(cè)泛素化蛋白質(zhì)水平評(píng)價(jià)泛素-蛋白酶體系統(tǒng)活性。蛋白免疫印跡法觀察自噬作用活性標(biāo)志蛋白LC3-Ⅱ的表達(dá)情況,共聚焦免疫熒光顯微鏡下觀察LC3、p62的表達(dá)與分布。蛋白免疫印跡法檢測(cè)p62表達(dá)的變化。結(jié)果在MG132作用后,晶狀體上皮細(xì)胞中泛素化蛋白聚集物明顯增加。隨著抑制時(shí)間的延長(zhǎng),LC3-Ⅱ的表達(dá)量也隨之增加,自噬作用活性增強(qiáng),p62的表達(dá)也明顯增加。結(jié)論在晶狀體上皮細(xì)胞中,當(dāng)泛素-蛋白酶體系統(tǒng)活性受抑制時(shí)自噬作用活性增強(qiáng)。
[Abstract]:Aim to investigate the effect of MG132 on autophagy and its mechanism in lens epithelial cells by inhibiting the activity of ubiquitin proteasome pathway. Methods the function of ubiquitin proteasome pathway was blocked by MG132, a protease inhibitor, and the activity of ubiquitin proteasome system was evaluated by Western blot. The expression of autophagy activity marker protein LC3- 鈪，

本文編號(hào)：2326147