【摘要】： 目的 重復性經(jīng)顱磁刺激刺激(repetitive transcranial magnetic stimulation, rTMS)是一種新型非創(chuàng)傷性的神經(jīng)科學研究工具,并可能成為多種精神疾病的治療手段。目前對rTMS的抗抑郁(樣)作用的性別差異研究較少,而且不同作用方式的rTMS對學習和記憶的影響尚不明確,另外rTMS的作用機制可能與神經(jīng)可塑性有關。因此,本實驗研究重復性經(jīng)顱磁刺激的生物效應,重點研究rTMS的抗抑郁樣作用及其長期rTMS抗抑郁樣作用的性別差異;并考察rTMS對學習和記憶的影響,研究短期高頻率rTMS對空間學習和記憶過程的影響,長期低頻率rTMS對學習和記憶的影響;同時研究長期rTMS對大鼠海馬Bcl-2蛋白表達的影響。 方法 Wistar雄、雌性大鼠接受rTMS連續(xù)作用10 d(15 Hz,1.0 Tesla,共2000次刺激)。以強迫游泳實驗觀察抗抑郁作用及其性別差異。Wistar雄性大鼠接受rTMS連續(xù)作用5 d,分別在第1 d和第5 d rTMS結束后接受曠場實驗。 本研究中,Wistar雄性大鼠接受高頻率rTMS(10 Hz,1.0 Tesla,200μs)300次刺激。使用Morris水迷宮實驗,考察訓練前或后給予rTMS處理對空間參考記憶和工作記憶的影響。
[Abstract]:Objective repetitive transcranial magnetic stimulation (repetitive transcranial magnetic stimulation, rTMS) is a new non-traumatic neuroscience tool and may be used as a therapeutic tool for various mental disorders. At present, there are few studies on the gender difference of antidepressant (like) effects of rTMS, and the effects of rTMS on learning and memory are not clear. In addition, the mechanism of rTMS may be related to neural plasticity. Therefore, we studied the biological effects of repetitive transcranial magnetic stimulation (TMS), focusing on the antidepressant effect of rTMS and the gender difference of long-term antidepressant effect of rTMS. The effects of rTMS on learning and memory, the effects of short-term high frequency rTMS on spatial learning and memory, long-term and low frequency rTMS on learning and memory, and the effects of long-term rTMS on the expression of Bcl-2 protein in hippocampus of rats were studied. Methods Wistar male and female rats were treated with rTMS for 10 days (15 Hz,1.0 Tesla, for 2000 times). The antidepressant effect and its gender difference were observed by forced swimming test. Wistar male rats were treated with rTMS for 5 days, and the open field test was performed on the 1st and 5th day after rTMS. In this study, Wistar male rats were stimulated with high frequency rTMS (10 Hz,1.0 Tesla,200 渭 s) 300 times. Morris water maze experiment was used to investigate the effects of rTMS treatment on spatial reference memory and working memory before or after training.
【學位授予單位】：四川大學
【學位級別】：博士
【學位授予年份】：2006
【分類號】：R312

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