O型口蹄疫病毒VP1蛋白的比較與牛O型表位肽基因工程疫苗的研制
發(fā)布時間：2018-11-05 12:34
【摘要】： 口蹄疫(Foot and mouth disease, FMD)是由口蹄疫病毒(Foot and mouth disease virus, FMDV)引起的主要侵害豬、牛等偶蹄動物的高度接觸傳染性疾病。在FMDV七個血清型中,O型是發(fā)現(xiàn)最早并流行最廣的型別之一。目前對其免疫控制的主要手段是滅活疫苗的接種,但有一定局限性,如成本高、接種量大及有散發(fā)活毒的危險。為此,我們釣取了O型FMDV流行株VP1的編碼基因,并結(jié)合中國及周邊國家病毒株序列的比較分析,在此基礎(chǔ)上,設(shè)計、構(gòu)建了兩株牛O型口蹄疫表位肽基因工程疫苗,并對其免疫效果進(jìn)行了檢測。 結(jié)果表明:一FMDV流行株的VP1基因與豬O型FMDV病毒株O/HKN/3/75的氨基酸同源性高達(dá)98%;O型FMDV西藏株(泛亞)VP1蛋白的RGD基序和G-H環(huán)的空間位置與文獻(xiàn)所報道的一致;構(gòu)建的新疆株牛O型蛋白疫苗和西藏株牛O型蛋白疫苗與豬O型FMDV疫苗免疫的陽性血清存在交叉識別;西藏株牛O型蛋白疫苗可在豚鼠誘生出西藏株口蹄疫病毒的特異性抗體。 本研究為研制更為有效的牛O型口蹄疫基因工程疫苗提供了可參考的實(shí)驗(yàn)依據(jù)。
[Abstract]:Foot-and-mouth disease (Foot and mouth disease, FMD) is a highly contagious disease caused by foot-and-mouth disease virus (Foot and mouth disease virus, FMDV), which mainly infects pigs, cattle and other cloven-hoofed animals. Among the seven serotypes of FMDV, type O is one of the earliest and most prevalent serotypes. At present, the main method of immunization control is inactivated vaccine, but it has some limitations, such as high cost, large amount of inoculation and risk of sporadic live virus. For this reason, we have isolated the coding gene of VP1 of type O FMDV epidemic strain, and combined with the comparative analysis of the sequence of virus strains in China and other countries, we have designed and constructed two strains of bovine foot-and-mouth disease epitope peptide gene engineering vaccine. The immune effect was tested. The results showed that the amino acid homology between the VP1 gene of a FMDV epidemic strain and O/HKN/3/75 of porcine type O FMDV virus was as high as 98%. The RGD motif and G-H ring of VP1 protein of O type FMDV Tibetan strain were consistent with those reported in the literature. The positive sera of Xinjiang bovine type O protein vaccine and Tibetan cattle type O protein vaccine immunized with porcine type O FMDV vaccine were cross-recognized, and Tibetan bovine type O protein vaccine could induce specific antibodies against the FMDV in guinea pigs. This study provides a reference experimental basis for the development of a more effective genetic engineering vaccine for bovine foot-and-mouth disease.
【學(xué)位授予單位】：吉林大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2007
【分類號】：R392
本文編號：2312099
[Abstract]:Foot-and-mouth disease (Foot and mouth disease, FMD) is a highly contagious disease caused by foot-and-mouth disease virus (Foot and mouth disease virus, FMDV), which mainly infects pigs, cattle and other cloven-hoofed animals. Among the seven serotypes of FMDV, type O is one of the earliest and most prevalent serotypes. At present, the main method of immunization control is inactivated vaccine, but it has some limitations, such as high cost, large amount of inoculation and risk of sporadic live virus. For this reason, we have isolated the coding gene of VP1 of type O FMDV epidemic strain, and combined with the comparative analysis of the sequence of virus strains in China and other countries, we have designed and constructed two strains of bovine foot-and-mouth disease epitope peptide gene engineering vaccine. The immune effect was tested. The results showed that the amino acid homology between the VP1 gene of a FMDV epidemic strain and O/HKN/3/75 of porcine type O FMDV virus was as high as 98%. The RGD motif and G-H ring of VP1 protein of O type FMDV Tibetan strain were consistent with those reported in the literature. The positive sera of Xinjiang bovine type O protein vaccine and Tibetan cattle type O protein vaccine immunized with porcine type O FMDV vaccine were cross-recognized, and Tibetan bovine type O protein vaccine could induce specific antibodies against the FMDV in guinea pigs. This study provides a reference experimental basis for the development of a more effective genetic engineering vaccine for bovine foot-and-mouth disease.
【學(xué)位授予單位】：吉林大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2007
【分類號】：R392
【參考文獻(xiàn)】
相關(guān)期刊論文 前2條
1 獨(dú)軍政,�；菔|,叢國正,林彤,邵軍軍,魏小娟,劉在新,謝慶閣;Asia1型口蹄疫病毒VP1基因的克隆、原核表達(dá)及純化[J];畜牧獸醫(yī)學(xué)報;2005年06期
2 劉光清,劉在新,謝慶閣;口蹄疫病毒持續(xù)性感染形成原因的探討[J];中國獸醫(yī)科技;2003年04期
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