發(fā)布時(shí)間：2018-10-26 12:36

【摘要】：目的探討臨床分離的對(duì)環(huán)丙沙星和左氧氟沙星均耐藥的銅綠假單胞菌耐藥機(jī)制。方法收集臨床分離經(jīng)VITEK-2(C0mpact細(xì)菌鑒定儀檢測(cè)環(huán)丙沙星和左氧氟沙星均耐藥的銅綠假單胞菌,瓊脂稀釋法測(cè)定環(huán)丙沙星和左氧氟沙星的MIC值,PCR擴(kuò)增DNA促旋酶基因gyrA和gyrB以及DNA拓?fù)洚悩?gòu)酶Ⅳ的parC和parE基因,實(shí)時(shí)-RT-PCR分析細(xì)菌外排系統(tǒng)表達(dá)情況。結(jié)果瓊脂稀釋法檢測(cè)結(jié)果與VITEK-2 Compact細(xì)菌鑒定儀檢測(cè)結(jié)果相符。PCR擴(kuò)增測(cè)序發(fā)現(xiàn)以DNA促旋酶基因gyrA(在位點(diǎn)941處插入堿基C)和gyrB(3株在位點(diǎn)1588處缺失堿基A,其他菌株在位點(diǎn)1543處插入堿基T)基因缺失或者插入導(dǎo)致移碼突變?yōu)橹?parE基因有3株在位點(diǎn)1895處插入堿基C。實(shí)時(shí)定量PCR檢測(cè)發(fā)現(xiàn)以mexA和mexC表達(dá)增加為主。結(jié)論檢出的耐環(huán)丙沙星和左氧氟沙星銅綠假單胞菌是由于DNA促旋酶基因gyrA和gyrB基因的突變和mexAB-OprM和mexCD-OprJ表達(dá)增加共同作用的結(jié)果。
[Abstract]:Objective to investigate the mechanism of resistance to ciprofloxacin and levofloxacin in clinical isolates of Pseudomonas aeruginosa. Methods Clinical isolates of Pseudomonas aeruginosa resistant to ciprofloxacin and levofloxacin were detected by VITEK-2 (C0mpact bacterial identification instrument). The MIC values of ciprofloxacin and levofloxacin were determined by Agar dilution method. The parC and parE genes of DNA promoter gene gyrA and gyrB and DNA topoisomerase 鈪，

本文編號(hào)：2295741