【摘要】：目的探討鷓鴣禽流感病毒和人流感病毒唾液酸受體分布特點及其作用。方法用親和組化法檢測鷓鴣呼吸道與消化道流感病毒唾液酸受體的分布,熒光素Alexa488標(biāo)記禽流感病毒H9N1和人流感病毒H1N1,分別與鷓鴣呼吸道、消化道各解剖部位組織細(xì)胞結(jié)合。結(jié)果鷓鴣呼吸道同時表達(dá)SAα-2,3Gal和SAα-2,6Gal兩種受體,但其消化道僅表達(dá)SAα-2,3Gal受體。SAα-2,3Gal受體在鷓鴣呼吸道各部位和消化道結(jié)腸上皮細(xì)胞呈強(qiáng)陽性彌漫分布;SAα-2,6Gal受體在呼吸道氣管、支氣管和次級支氣管呈強(qiáng)陽性彌漫分布,而副支氣管、消化道結(jié)腸均為缺乏或僅表達(dá)極少量。H9N1禽流感病毒與鷓鴣呼吸道各部位和消化道結(jié)腸均結(jié)合;人流感病毒H1N1與鷓鴣的氣管、支氣管、次級支氣管結(jié)合,但未見與副支氣管和結(jié)腸結(jié)合,此結(jié)果與鷓鴣呼吸道和消化道SAα-2,3Gal和SAα-2,6Gal受體分布總體一致。結(jié)論?dān)p鴣同時表達(dá)SAα-2,3Gal和SAα-2,6Gal受體,能與禽流感病毒和人流感病毒結(jié)合,有助于禽流感病毒與人流感病毒基因重配,提示其可充當(dāng)流感病毒潛在的中間宿主。
[Abstract]:Objective to investigate the distribution and function of sialic acid receptors of partridge avian influenza virus and human influenza virus. Methods the distribution of sialic acid receptor (sialic acid receptor) of influenza virus in respiratory tract and digestive tract of partridge was detected by affinity histochemical method. H9N1 of avian influenza virus and H1 1 of human influenza virus were labeled with fluorescein Alexa488 and combined with tissues and cells of respiratory tract and digestive tract of partridge respectively. Results both SA 偽 -2Gal and SA 偽 -2Gal were expressed in the respiratory tract of Partridge, but only SA 偽 -2O3Gal receptor was expressed in the digestive tract. SA 偽 -2O3Gal receptor was strongly distributed in the colonic epithelium of the partridge respiratory tract and the colon epithelium of the digestive tract, and SA 偽 -26Gal receptor was found in the trachea of the partridge trachea. The bronchi and secondary bronchi were strongly positive and diffuse, while the accessory bronchus and digestive tract colon were deficient or only expressed a little. H9N1 avian influenza virus combined with partridge respiratory tract and digestive tract colon. Human influenza virus (H1N1) binds to the trachea, bronchi and secondary bronchi of partridges, but not to the accessory bronchi and colon. The results are consistent with the distribution of SA 偽 -2Gal and SA 偽 -2O6Gal receptors in the respiratory tract and digestive tract of Partridge. Conclusion Partridge expresses both SA 偽 -2Gal and SA 偽 -2Gal receptors, which can bind to avian influenza virus and human influenza virus, which is helpful to rematch the gene between avian influenza virus and human influenza virus, suggesting that the partridge can act as a potential intermediate host of influenza virus.
【作者單位】： 廣西醫(yī)科大學(xué)微生物學(xué)教研室;
【基金】：國家自然科學(xué)基金(No.30960345,No.31260033) 廣西自然科學(xué)基金(No.2013GXNSFAA019118) 廣西科學(xué)研究與技術(shù)開發(fā)計劃課題(桂科No.1298014-23) 廣西醫(yī)學(xué)科學(xué)實驗中心開放基金項目(No.KFJJ2010-29) 廣西研究生教育創(chuàng)新計劃項目(No.YCSZ20120033)聯(lián)合資助~~
【分類號】：R373.13

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