【摘要】： 紅色毛癬菌是重要的淺表感染真菌。世界范圍內(nèi),60%的淺部真菌病由該菌引起。本文構(gòu)建了紅色毛癬菌BMU01672的cDNA文庫,并進(jìn)行大規(guī)模測序。通過聚類分析獲得了10188條無冗余基因表達(dá)序列標(biāo)簽(Expression Sequence Tags, EST)。通過與現(xiàn)有數(shù)據(jù)庫進(jìn)行比較和利用COG軟件對這些EST進(jìn)行功能分類,明確了部分基因的功能以及代謝途徑。 為了進(jìn)一步研究紅色毛癬菌的的生理過程、致病機(jī)制和代謝特點(diǎn),我們構(gòu)建了包含10250條紅色毛癬菌EST的cDNA芯片。并用此對紅色毛癬菌的孢子萌發(fā)過程進(jìn)行了研究。根據(jù)實(shí)驗(yàn)觀察和文獻(xiàn)報(bào)道,我們將紅色毛癬菌孢子萌發(fā)過程分為16個(gè)時(shí)相,分別提取其mRNA作芯片雜交。 在對所得數(shù)據(jù)進(jìn)行篩選后,總共獲得共1576個(gè)有效數(shù)據(jù)。對這1576個(gè)基因進(jìn)行聚類分析,使用K-means聚類,最后選擇的聚類clusters數(shù)目為5,經(jīng)過SDSS 10.0統(tǒng)計(jì)軟件驗(yàn)證,證明了聚類間的統(tǒng)計(jì)學(xué)意義。 對紅色毛癬菌在孢子生長過程中的基因表達(dá)變化和具體的生理進(jìn)程之間的關(guān)系進(jìn)行研究。根據(jù)GO數(shù)據(jù)庫分類,對實(shí)驗(yàn)中所獲得的差異基因進(jìn)行了分類研究,以對不同功能的基因在孢子萌發(fā)時(shí)可能的作用進(jìn)行分析。 同時(shí)將這1576個(gè)ESTs與酵母基因數(shù)據(jù)庫(the Saccharomyces Genome Database,SGD)進(jìn)行了對比研究,通過比對同源基因在紅色毛癬菌孢子生長過程中表達(dá)量的變化,分別對這些信號通路在紅色毛癬菌孢子萌發(fā)中的作用進(jìn)行了分析。
[Abstract]:Trichophyton rubrum is an important superficial infective fungus. Worldwide, 60% of superficial mycosis is caused by this fungus. The cDNA library of Trichophyton rubrum BMU01672 was constructed and sequenced on a large scale. 10188 non-redundant gene expression sequence tags (Expression Sequence Tags, EST). Were obtained by cluster analysis. By comparing with existing databases and using COG software to classify the functions of these EST, the functions and metabolic pathways of some genes were clarified. In order to further study the physiological process, pathogenic mechanism and metabolic characteristics of Trichophyton rubrum, we constructed a cDNA microarray containing 10250 EST of Trichophyton rubrum. The spore germination process of Trichophyton rubrum was studied. According to the experimental observation and literature reports, we divided the spore germination process of Trichophyton rubrum into 16 time phases and extracted its mRNA for microarray hybridization. After screening, a total of 1576 valid data were obtained. The 1576 genes were clustered by K-means, and the number of cluster clusters was 5, which was verified by SDSS 10.0 statistical software, which proved the statistical significance of clustering. The relationship between gene expression and physiological process of Trichophyton rubrum during spore growth was studied. According to the classification of GO database, the differential genes obtained in the experiment were classified and studied to analyze the possible role of genes with different functions in spores germination. At the same time, 1576 ESTs were compared with yeast gene database (the Saccharomyces Genome Database,SGD. By comparing the expression of homologous genes in the spore growth process of Trichophyton rubrum, we compared the expression levels of homologous genes in the spores of Trichophyton rubrum. The effects of these signaling pathways on spore germination of Trichophyton rubrum were analyzed.
【學(xué)位授予單位】：吉林大學(xué)
【學(xué)位級別】：博士
【學(xué)位授予年份】：2007
【分類號】：R346

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