【摘要】：在胚胎早期神經(jīng)營養(yǎng)素-3(neurotrophin-3，NT-3)對神經(jīng)元的存活、突起生長及靶選擇發(fā)揮著重要的作用，隨著胚胎的發(fā)育其表達逐漸減少，功能作用也隨之減弱。在成年動物神經(jīng)損傷后，NT-3的表達再次增高，，參與到損傷修復(fù)的過程中。有實驗顯示成年貓部分背根損傷后備用背根節(jié)中NT-3mRNA表達上調(diào)，同時保留的備用背根在脊髓內(nèi)發(fā)生了側(cè)支出芽，以代償丟失的初級傳入纖維，提示增多的NT-3很可能參與了上述的代償、修復(fù)過程。 為了探明成年貓正常背根節(jié)和部分去背根后備用背根節(jié)內(nèi)源性NT-3對節(jié)內(nèi)神經(jīng)元的作用和作用模式，NT-3對備用背根側(cè)支出芽的影響。以及背根節(jié)內(nèi)與NT-3功能可能有相關(guān)關(guān)系的神經(jīng)肽的表達及作用，本文采用CTB神經(jīng)束路追蹤、分離神經(jīng)元培養(yǎng)、免疫組織化學(xué)、免疫熒光、酶聯(lián)免疫吸附等技術(shù)從三個方面進行研究：NT-3促背根節(jié)神經(jīng)突起生長作用探討；備用背根節(jié)內(nèi)NT-3表達的變化及作用模式探討：背根節(jié)神經(jīng)元內(nèi)NT-3與肽類物質(zhì)的共存及相關(guān)作用的探討。
[Abstract]:Neurotrophin-3 (neurotrophin-3,NT-3) plays an important role in neuronal survival, process growth and target selection in early embryonic development. After nerve injury in adult animals, the expression of NT-3 was increased again and involved in the process of injury and repair. Some experiments showed that the expression of NT-3mRNA was up-regulated in the spare dorsal root ganglion after partial dorsal root injury in adult cats, while the reserved dorsal root developed lateral sprouts in the spinal cord to compensate for the lost primary afferent fibers. It is suggested that increased NT-3 may be involved in the above compensatory and repair process. In order to investigate the effect and mode of endogenous NT-3 on neurons in normal dorsal root ganglion (DRG) and partial dorsal root ganglia (DRG) of adult cats, the effect of NT-3 on spare dorsal root outgrowth bud was studied. The expression and role of neuropeptides in dorsal root ganglion which may be related to NT-3 function were studied by CTB nerve tract tracing, neuronal culture, immunohistochemistry and immunofluorescence. Enzyme-linked immunosorbent assay (Elisa) was used to study the effects of NT-3 on the growth of dorsal root ganglion (DRG) neurites. The changes of NT-3 expression in dorsal root ganglion and its mechanism of action: the coexistence of NT-3 and peptide in dorsal root ganglion neurons and the correlation between them.
【學(xué)位授予單位】：四川大學(xué)
【學(xué)位級別】：博士
【學(xué)位授予年份】：2005
【分類號】：R322

【參考文獻】

相關(guān)期刊論文 前6條

1 王廷華,吳良芳,廖德陽,周雪,陳彥紅,A.Takeda;針刺對神經(jīng)營養(yǎng)素3在脊髓可塑性中表達的影響[J];華西醫(yī)科大學(xué)學(xué)報;2002年01期

2 王廷華,劉芬,張曉,王特為,柯青,周雪,吳良芳;部分背根切斷對備用背根節(jié)NT-3表達的影響[J];解剖學(xué)報;2003年06期

3 陳麗華,吳良芳,周雪;腦源性神經(jīng)營養(yǎng)因子在部分去傳入貓脊髓背角的表達[J];神經(jīng)解剖學(xué)雜志;2000年03期

4 龍雙漣,吳良芳,保天然;部分去背根傳入貓脊髓Ⅱ板層神經(jīng)生長因子的免疫組化研究[J];神經(jīng)解剖學(xué)雜志;1997年04期

5 鄒曉菊;吳良芳;;貓后肢背根全切后背核突觸性終末的可塑性—電鏡定量研究[J];神經(jīng)解剖學(xué)雜志;1993年01期

6 周雪,吳良芳,王廷華,諶宏鳴,保天然,廖德陽;針刺對部分去背根貓脊髓背角與備用背根節(jié)神經(jīng)營養(yǎng)素家族及其mRNA表達的影響[J];中國針灸;2002年11期

本文編號：2267751