面向微流控芯片的微量血清分離方法的研究
發(fā)布時間：2018-10-09 15:49
【摘要】： 為了滿足微全分析系統(tǒng)對樣品分離的集成度要求,研究一種新型的微量血清分離技術(shù)已成為一個急待解決的問題。論文在查閱大量國內(nèi)外相關(guān)資料的基礎(chǔ)上提出采用微過濾法和離心分離的基本方案,并結(jié)合血清分離的特殊性,對微血清分離器進(jìn)行了理論分析、器件制作及實驗研究。 在研究微過濾理論的基礎(chǔ)上,利用MEMS技術(shù)設(shè)計制作了一種條柵狀的硅微結(jié)構(gòu)的過濾器,并對其進(jìn)行了實驗研究。結(jié)果表明,該過濾器能有效去除懸液中直徑14μm以上的微粒,但對具有較強(qiáng)變形能力的紅細(xì)胞過濾不徹底。 對毛細(xì)管離心分離進(jìn)行了原理研究和實驗分析。在此基礎(chǔ)上設(shè)計并制作了兩種不同封裝形式的離心式血清微分離芯片——全PDMS血清微分離芯片和硅-PDMS混合式血清微分離芯片,實現(xiàn)了微升量級血清的分離。文中分別介紹了兩種芯片的制作工藝,并對其性能進(jìn)行了實驗分析。分析討論了材料表面性質(zhì)、微通道管徑和離心運行參數(shù)對血清分離結(jié)果的影響。分析討論了硅模表面粗糙度和PDMS固化溫度對芯片鍵合強(qiáng)度的影響。 為了解決血清分離后的取樣問題,在離心式分離芯片的基礎(chǔ)上,將微閥和氣泵進(jìn)行了集成設(shè)計。利用改變表面張力可控制流體在微通道的流動的原理,設(shè)計了實現(xiàn)血液自動進(jìn)樣的毛細(xì)微泵;通過改變微通道幾何尺寸的實驗,設(shè)計了可使流體在通道狹窄處止流的毛細(xì)微閥。閥的操作模式表明,此類被動閥適合于在低壓、一次性使用的場合。
[Abstract]:In order to meet the requirement of integration of microanalysis system for sample separation, it has become an urgent problem to study a new microserum separation technology. On the basis of consulting a large number of related data at home and abroad, this paper puts forward the basic scheme of microfiltration and centrifugal separation, and combines the particularity of serum separation, carries on the theoretical analysis, the device fabrication and the experimental research to the microserum separator. On the basis of studying the theory of microfiltration, a kind of silicon microstructure filter with strip gate is designed and fabricated by using MEMS technology, and its experimental study is carried out. The results show that the filter can effectively remove the particles over 14 渭 m in diameter in the suspension, but it is not complete for the red blood cells with strong deformability. The principle and experimental analysis of capillary centrifugation were studied. On the basis of this, two kinds of centrifugal serum microseparation chips, full PDMS serum microseparation chip and silicon-PDMS mixed serum microseparation chip were designed and fabricated, which realized the separation of microserum in the order of magnitude. In this paper, the fabrication process of two kinds of chips are introduced, and their performances are analyzed experimentally. The effects of material surface properties, microchannel diameter and centrifugal operation parameters on serum separation results were analyzed and discussed. The effects of surface roughness and PDMS curing temperature on bonding strength of silicon die were analyzed and discussed. In order to solve the problem of sampling after serum separation, the microvalve and air pump were designed on the basis of centrifugal separation chip. Based on the principle that the fluid can be controlled by changing the surface tension in the microchannel, a capillary pump is designed to realize the automatic injection of blood, and by changing the geometric dimension of the microchannel, the capillary fine valve is designed to stop the fluid from flowing at the narrow point of the channel. The operating mode of the valve indicates that this type of passive valve is suitable for low pressure, one-time use.
【學(xué)位授予單位】：中國科學(xué)院研究生院（長春光學(xué)精密機(jī)械與物理研究所）
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2006
【分類號】：R-331
本文編號：2259893
[Abstract]:In order to meet the requirement of integration of microanalysis system for sample separation, it has become an urgent problem to study a new microserum separation technology. On the basis of consulting a large number of related data at home and abroad, this paper puts forward the basic scheme of microfiltration and centrifugal separation, and combines the particularity of serum separation, carries on the theoretical analysis, the device fabrication and the experimental research to the microserum separator. On the basis of studying the theory of microfiltration, a kind of silicon microstructure filter with strip gate is designed and fabricated by using MEMS technology, and its experimental study is carried out. The results show that the filter can effectively remove the particles over 14 渭 m in diameter in the suspension, but it is not complete for the red blood cells with strong deformability. The principle and experimental analysis of capillary centrifugation were studied. On the basis of this, two kinds of centrifugal serum microseparation chips, full PDMS serum microseparation chip and silicon-PDMS mixed serum microseparation chip were designed and fabricated, which realized the separation of microserum in the order of magnitude. In this paper, the fabrication process of two kinds of chips are introduced, and their performances are analyzed experimentally. The effects of material surface properties, microchannel diameter and centrifugal operation parameters on serum separation results were analyzed and discussed. The effects of surface roughness and PDMS curing temperature on bonding strength of silicon die were analyzed and discussed. In order to solve the problem of sampling after serum separation, the microvalve and air pump were designed on the basis of centrifugal separation chip. Based on the principle that the fluid can be controlled by changing the surface tension in the microchannel, a capillary pump is designed to realize the automatic injection of blood, and by changing the geometric dimension of the microchannel, the capillary fine valve is designed to stop the fluid from flowing at the narrow point of the channel. The operating mode of the valve indicates that this type of passive valve is suitable for low pressure, one-time use.
【學(xué)位授予單位】：中國科學(xué)院研究生院（長春光學(xué)精密機(jī)械與物理研究所）
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2006
【分類號】：R-331
【引證文獻(xiàn)】
相關(guān)博士學(xué)位論文 前1條
1 渠柏艷;基于血液樣品和微流控芯片技術(shù)的細(xì)胞分離、PCR擴(kuò)增及DNA測序方法研究[D];東北大學(xué);2009年
，本文編號：2259893
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