【摘要】： 目的:用環(huán)磷酰胺腹腔注射造成免疫抑制小鼠動(dòng)物模型,研究青春雙歧桿菌在體內(nèi)對(duì)免疫抑制小鼠的免疫增強(qiáng)作用。方法:先用環(huán)磷酰胺給正常小鼠腹腔注射連續(xù)4天,出現(xiàn)免疫抑制狀態(tài)后,再用青春雙歧桿菌菌液3種不同濃度干預(yù)治療免疫抑制小鼠,同時(shí)設(shè)黃芪治療組,正常生理鹽水對(duì)照組和單純免疫抑制組,每組又分為7天治療和14天治療,分別測(cè)各組小鼠血中IL-2含量,胸腺、脾臟、肝臟指數(shù),小鼠體重增長(zhǎng)曲線,糞便青春雙歧桿菌(Bifido adolescentis,BFA)菌落計(jì)數(shù),血白細(xì)胞數(shù)、胸腺、肝、脾HE梁色切片,采用SPSS12.0統(tǒng)計(jì)軟件包進(jìn)行數(shù)據(jù)處理分析,統(tǒng)計(jì)學(xué)方法采用雙因素重復(fù)測(cè)量資料的方法分析單因素隨機(jī)化設(shè)計(jì)的方差分析,卡方檢驗(yàn),多組均數(shù)比較的非參數(shù)秩和檢驗(yàn)。結(jié)果:隨著治療天數(shù)的增加,BFA糞便菌落數(shù)逐漸減少,雙歧桿菌高劑量組、生理鹽水組、單純免疫抑制組比較有統(tǒng)計(jì)學(xué)意義,提示BFA隨攝入增多,腸道定植也增多;根據(jù)肝、脾、胸腺指數(shù)的比較結(jié)果,說(shuō)明BFA對(duì)免疫器官有一定增重作用;血IL-2,白細(xì)胞含量比較,BFA高劑量組最高,提示雙歧桿菌對(duì)免疫抑制小鼠免疫細(xì)胞分泌IL-2,以及白細(xì)胞生成有一定促進(jìn)作用,體重曲線也表現(xiàn)出高劑量組雙歧桿菌對(duì)體重促進(jìn)作用最明顯,組織HE染色形態(tài)觀察也提示雙歧桿菌對(duì)胸腺、肝、脾免疫淋巴組織有恢復(fù)作用。結(jié)論:雙歧桿菌對(duì)環(huán)磷酰胺免疫抑制小鼠有一定的免疫增強(qiáng)作用。
[Abstract]:Aim: to establish an immunosuppressive mouse model by intraperitoneal injection of cyclophosphamide and to study the immunoenhancement effect of Bifidobacterium pubertidis on immunosuppressive mice in vivo. Methods: normal mice were injected intraperitoneally with cyclophosphamide for 4 days, and then treated with three different concentrations of Bifidobacterium pubescens solution. The mice were treated with astragalus membranaceus (Astragalus membranaceus) at the same time. Normal saline control group and immunosuppressive group, each group was divided into 7 days treatment and 14 days treatment. Blood IL-2 content, thymus, spleen, liver index, weight growth curve of mice in each group were measured, respectively. Fecal Bifido adolescentis bacillus colony count, white blood cell count, thymus, liver, spleen HE color sections were analyzed by SPSS12.0 statistical software package. Statistical methods were used to analyze the ANOVA, chi-square test, and nonparametric rank sum test of multivariate mean comparison in single factor randomization design. Results: with the increase of treatment days, the fecal colony number of BFA gradually decreased. There were significant differences among the high dose bifidobacterium group, normal saline group and immunosuppressive group, suggesting that BFA increased with the intake, intestinal colonization also increased, according to liver, spleen, The comparison of thymus index indicated that BFA had a certain weight gain effect on immune organs, and the contents of IL-2 and WBC were the highest in high dose group. It was suggested that Bifidobacterium could promote the secretion of IL-2 and leukocyte formation in immunosuppressive mice, and the body weight curve showed that Bifidobacterium in high dose group had the most obvious effect on body weight. The morphological observation of HE staining also showed that Bifidobacterium could recover the immune lymphoid tissue of thymus, liver and spleen. Conclusion: Bifidobacterium has a certain immunoenhancement effect on cyclophosphamide immunosuppressive mice.
【學(xué)位授予單位】：新疆醫(yī)科大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2007
【分類號(hào)】：R371

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