【摘要】： 目的一般認為長壽的機理是多種因素、綜合作用的結果,不是單一因素所決定的。人類的長壽現(xiàn)象更多的是一個體與外界的生存環(huán)境相適應的結果。在與長壽有關的因素中,遺傳因素無疑是最重要或最主要的因素之一。人類基因組計劃的研究表明,人類基因中99.9%的遺傳密碼DNA都是一樣的,只有大約0.1%的基因不同,具有個體差異。因此,與人類長壽有關的遺傳因素很可能與這0.1%個體差異相關。目前,人類最大的遺傳多態(tài)性是人類白細胞抗原系統(tǒng)(Human Leukocyte Antigen, HLA)和微衛(wèi)星DNA(short tandem repeat,STR)。因此本文選定HLA-I類基因和15個STR系統(tǒng)的等位基因,旨在研究與人類長壽相關的遺傳因素。 方法選取大連地區(qū),年齡在90歲以上的男性老人18人,作為觀察組。對照組隨機選取大連地區(qū)無償獻血志愿者男性50名,身體健康,體檢標準符合“全國采供血管理規(guī)范”。家族無遺傳病史,年齡在18-55周歲。 樣本采集,采靜脈血2ml,EDTA抗凝。HLA-A、B基因分型方法,采用SSO方法。首先,提取DNA,進行PCR擴增。然后,按試劑盒說明書進行變性與中和后再與磁珠雜交,用SAPE染色,洗滌后,讀板,計算機自動按順序讀取數(shù)據(jù)并分析出A、B、DR的特異性。 STR檢測采用Identifiler PlusTM (ABI公司,美國)試劑盒,擴增位點為D8S1179、D21S11、D7S820、CSF1PO、D3S1358、THO1、D13S317、D16S539、D2S1338、D19S433、VWA、TPOX、D18S51、D5S818、FGA和Amelogenin性別位點;采用310型DNA
[Abstract]:Objectives It is generally believed that the mechanism of longevity is determined by many factors and not by a single factor. Human longevity is more likely to be the result of adaptation to the external environment. Among the factors related to longevity, genetic factors are undoubtedly one of the most important or most important. Studies have shown that 99.9% of the genetic code DNA in human genes is the same, only about 0.1% of the genes are different and have individual differences. And microsatellite DNA (short tandem repeat, STR). Therefore, HLA-I and 15 STR alleles were selected to study the genetic factors related to human longevity.
Methods Eighteen male elderly people over 90 years old in Dalian area were selected as the observation group. Fifty male volunteers in Dalian area were randomly selected as the control group.
Sample collection, venous blood 2 ml, EDTA anticoagulant, HLA-A, B genotyping method, using SSO method. First, extract DNA, PCR amplification. Then, according to the kit instructions for denaturation and neutralization and then hybridization with magnetic beads, stained with SAPE, washed, read the board, computer automatically read the data in order to analyze the specificity of A, B, DR.
STR was detected by Identifiler PlusTM (ABI Company, USA) kit. The amplified sites were D8S1179, D21S11, D7S820, CSF1PO, D3S1358, THO1, D13S317, D16S539, D2S1338, D19S433, VWA, TPOX, D18S51, D5S818, FGA and Amelogenen. Gender loci 310 were used.
【學位授予單位】：大連醫(yī)科大學
【學位級別】：碩士
【學位授予年份】：2006
【分類號】：R394

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