【摘要】：目的 探討不同濃度的羥乙基淀粉類血漿代用品(賀斯,6%HAES 200/0.5)對正常人體免疫系統(tǒng)白介素-2,10,12(IL-2,10,12)及干擾素-γ(IFN-γ)的影響,以檢驗其是否抑制正常人體的免疫系統(tǒng),為在圍術(shù)期替代輸血提供試驗依據(jù),以降低輸血造成的惡性腫瘤的復(fù)發(fā)率及術(shù)后感染率和病死率。 方法 正常志愿者12名,嚴(yán)格無菌條件下抽取靜脈血各20毫升,并以肝素抗凝,在超凈臺內(nèi)分離出單個核細(xì)胞,計數(shù)后將細(xì)胞平均分成5組:1640培養(yǎng)液陰性對照組、10%血漿對照組、10%賀斯試驗組、20%賀斯試驗組、40%賀斯試驗組,然后再將各組分成陰性對照組、加脂多糖(LPS)組、加植物血凝素(PHA)組,使單個核細(xì)胞在不同的培養(yǎng)液中培養(yǎng)48小時后,離心提取上清液,以酶聯(lián)免疫吸附法(ELISA法)檢測各上清液中細(xì)胞因子白介素-2,10,12(IL-2,IL-10,IL-12)和干擾素-γ(IFN-γ)的濃度。 結(jié)果 三個濃度的賀斯試驗組與1640培養(yǎng)液及血漿對照組在三種環(huán)境(陰性對照、加LPS、加PHA)中比較,雖然四種細(xì)胞因子的濃度值稍有參差,但統(tǒng)計學(xué)顯示無顯著性差異(p0.05);在IL-2和IFN-γ,陰性對照組與加LPS組無顯著性差異(p0.05),而兩者均與加PHA組有顯著性差異(p0.05);在IL-10,IL-12,陰性對照組、加LPS組及加PHA組之間均示有顯著性差異(p0.05)。 結(jié)論 三個濃度的賀斯下培養(yǎng)單個核細(xì)胞48小時后與同條件下的血漿組及陰性培養(yǎng)液組的細(xì)胞因子濃度無顯著差異,提示三個濃度的賀斯在體外環(huán)境中不影響免疫細(xì)胞的生存、克隆及細(xì)胞因子的產(chǎn)生,即:賀斯在體外試驗
[Abstract]:Objective to investigate the effects of different concentrations of hydroxyethyl starch plasma substitutes (HES 200 / 0.5) on the levels of interleukin-2siloxacin 1012 (IL-2m10N12) and interferon- 緯 (IFN- 緯) in normal human immune system in order to determine whether they inhibit the immune system of normal human beings. In order to reduce the recurrence rate, postoperative infection rate and mortality of malignant tumor caused by blood transfusion, we can provide experimental basis for replacing blood transfusion in perioperative period. Methods Mononuclear cells were isolated from 12 normal volunteers under strict aseptic conditions by extracting 20 ml of venous blood and using heparin as anticoagulant. After counting, the cells were divided into 5 groups, 10% plasma control group, 10% Horse test group and 40% Horse test group, then each group was divided into negative control group, lipopolysaccharide (LPS) group and phytohemagglutinin (PHA) group. After the mononuclear cells were cultured in different culture medium for 48 hours, the supernatants were extracted by centrifugation. The concentrations of cytokines interleukin-210-10 (IL-12) and interferon- 緯 (IFN- 緯) in the supernatants were detected by enzyme-linked immunosorbent assay (ELISA). Results the concentrations of four cytokines were slightly different between the three concentrations of Horse test group and 1640 medium and plasma control group in three environments (negative control, plus LPS, plus PHA), although the concentrations of four cytokines were slightly different. In IL-2 and IFN- 緯, there was no significant difference between the negative control group and the LPS group (p0.05), but there was a significant difference between the two groups (p0.05), and there was a significant difference between the IL-10 IL-12 group, the negative control group, the LPS group and the PHA group (p0.05). Conclusion there was no significant difference in cytokine concentrations between the three concentrations of Hos cultured mononuclear cells in vitro and plasma and negative medium groups after 48 hours, suggesting that the three concentrations of Hos did not affect the survival of immune cells in vitro. Cloning and production of cytokines, I. e., Hoss in vitro
【學(xué)位授予單位】：天津醫(yī)科大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2005
【分類號】：R392.1

【參考文獻(xiàn)】

相關(guān)期刊論文 前10條

1 董曉軍;白細(xì)胞介素-10檢測及其臨床意義[J];標(biāo)記免疫分析與臨床;2001年04期

2 王珊娟;血漿代用品的臨床應(yīng)用進(jìn)展[J];國外醫(yī)學(xué).麻醉學(xué)與復(fù)蘇分冊;1995年02期

3 徐雪;人工血漿代用品對凝血功能的影響[J];國外醫(yī)學(xué).麻醉學(xué)與復(fù)蘇分冊;2001年02期

4 徐從高,管增偉;IL-12抗腫瘤活性的研究進(jìn)展[J];國外醫(yī)學(xué)(腫瘤學(xué)分冊);1996年06期

5 袁世熒,姚尚龍,夏磊銘,衛(wèi)富強;羥乙基淀粉溶液對手術(shù)病人血漿細(xì)胞因子的影響[J];臨床麻醉學(xué)雜志;2002年08期

6 鄧碩曾,劉進(jìn);建議中華麻醉學(xué)會制定輸血指南[J];臨床麻醉學(xué)雜志;1999年03期

7 李文碩;血漿代用品的臨床應(yīng)用[J];天津護理;2002年05期

8 王軍,任久林,魏繼東;白細(xì)胞介素與心血管疾病研究進(jìn)展[J];醫(yī)學(xué)綜述;2002年12期

9 馬騏,張濤;最新一代血漿代用品——中分子羥乙基淀粉(賀斯)[J];The Chinese-German Journal of Clinical Oncology;1999年04期

10 張進(jìn)玉，，楊世勇;細(xì)胞因子與臨床[J];中級醫(yī)刊;1996年01期

本文編號：2162499