【摘要】：目的： 1．確認(rèn)在大鼠卵巢卵泡膜中有肌成纖維細(xì)胞存在 2．探討肌成纖維細(xì)胞與卵巢排卵的關(guān)系 方法： 1．采用免疫組織化學(xué)方法檢測(cè)α-SMA、desmin、vimentin、ET-AR和ET-BR在動(dòng)情期組(n=20)與非動(dòng)情期組(n=10)大鼠卵巢各級(jí)卵泡(竇前卵泡、小竇狀卵泡和大竇狀卵泡)卵泡膜細(xì)胞中的表達(dá)情況，并進(jìn)行圖像分析。 2．采用透射電鏡技術(shù)觀察動(dòng)情期大鼠竇狀卵泡卵泡膜細(xì)胞的超微結(jié)構(gòu)。 結(jié)果： 1．免疫組織化學(xué)結(jié)果： 1．1．α-SMA表達(dá)于動(dòng)情期和非動(dòng)情期各級(jí)卵泡的卵泡膜細(xì)胞中，其表達(dá)強(qiáng)度在組間同級(jí)卵泡間比較有顯著性差異(P＜0.05)，組內(nèi)各級(jí)卵泡間比較也有顯著性差異(P＜0.05)；在動(dòng)情期組其表達(dá)強(qiáng)度與卵泡直徑大小成正相關(guān)(r=0.631)，而非動(dòng)情期組兩者卻幾乎無(wú)相關(guān)性(r=-0.087)。 1．2．Desmin在動(dòng)情期組各級(jí)卵泡的卵泡膜細(xì)胞中表達(dá)，其表達(dá)強(qiáng)度在該組各級(jí)卵泡間比較有顯著性差異(P＜0.05)：在非動(dòng)情期組各級(jí)卵泡的卵泡膜細(xì)胞中無(wú)表達(dá)。 1．3．Vimentin表達(dá)于動(dòng)情期和非動(dòng)情期各級(jí)卵泡的卵泡膜細(xì)胞中，其表達(dá)強(qiáng)度在組間同級(jí)卵泡間比較有顯著性差異(P＜0.05)。動(dòng)情期組各級(jí)卵泡間比較有顯著性差異(P＜0.05)而且vimentin表達(dá)強(qiáng)度與卵泡直徑大小呈正相關(guān)(r=0.536)；非動(dòng)情期組竇前卵泡和小竇狀卵泡間比較無(wú)明顯差異(P＞0.05)，其他級(jí)別卵泡間比較有顯著性差異(P＜0.05)，而且vimentin表達(dá)強(qiáng)度與卵泡
[Abstract]:Objective: 1. To confirm the presence of myofibroblasts in the follicular membrane of rat ovary 2. To investigate the relationship between myofibroblasts and ovulation. 1.Immunohistochemical method was used to detect the ovarian follicles (preantral follicles) in the estrus group (nong20) and the non-estrous group (nm10). The expression of follicular membrane cells in antral follicles and large antral follicles, The ultrastructure of antral follicular membrane cells in estrus was observed by transmission electron microscopy. Results: 1. Immunohistochemical results: 1.1. 偽 -SMA was expressed in follicular membrane cells of all levels of follicles in estrus and non-estrous stage. There was a significant difference in the expression intensity between the same grade follicles (P < 0.05) and among the follicles of different levels in the group (P < 0.05). There was a positive correlation between the expression intensity and follicle diameter in estrus group (r 0. 631), but almost no correlation between the expression intensity and follicle diameter in non estrus group (r 0. 087). 1.2.Desmin was active in oestrus. In the oestrous stage group, the follicular membrane cells of all levels of follicles were expressed, There was significant difference in the expression intensity among the follicles (P < 0.05), and there was no expression in the theca cells of the follicles in the non-estrous period group. 1.3.Vimentin was expressed in follicular membrane cells of all levels of follicles in estrus and non-estrous stage. There was significant difference in expression intensity between the same grade follicles (P < 0.05). In estrus group, there was significant difference among follicles (P < 0. 05), and there was a positive correlation between vimentin expression and follicular diameter (r = 0. 536). There was no significant difference between preantral follicle and antral follicle in non-estrous stage group (P > 0. 05), but there was significant difference between other grade follicles (P < 0. 05), and the expression intensity of vimentin was higher than that of follicle group (P < 0. 05).
【學(xué)位授予單位】：暨南大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2006
【分類號(hào)】：R321

【參考文獻(xiàn)】

相關(guān)期刊論文 前4條

1 申洪;免疫組織化學(xué)染色定量方法研究（Ⅲ）[J];中國(guó)組織化學(xué)與細(xì)胞化學(xué)雜志;1995年01期

2 蔣永亮,戴愛國(guó);成肌纖維細(xì)胞與肺部疾病[J];國(guó)外醫(yī)學(xué).呼吸系統(tǒng)分冊(cè);2004年03期

3 徐斯凡，趙白鴿，周壽康，王忠興，，趙偉，吳曉云;內(nèi)皮素－1在大鼠卵巢內(nèi)的分布[J];江西醫(yī)學(xué)院學(xué)報(bào);1994年01期

4 劉英開,陸樹良;力學(xué)刺激對(duì)肌成纖維細(xì)胞轉(zhuǎn)歸的影響[J];中華創(chuàng)傷雜志;2002年11期

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