本文選題：L-精氨酸　切入點：暴發(fā)性胰腺炎　出處：《汕頭大學(xué)》2006年碩士論文

【摘要】：背景目的：暴發(fā)性胰腺炎(fulminant acute pancreatitis，F(xiàn)AP)作為一種特殊類型的胰腺炎(acute pancreatitis，AP)，其臨床特點是發(fā)病迅速、來勢兇險、早期即可出現(xiàn)多器官功能不全、死亡率高。雖然目前臨床上常有這類胰腺炎病因、治療等方面的報道，但國內(nèi)外對FAP以及胰外器官損傷情況研究極少，認識也極其有限。因此，建立FAP動物模型，進而對FAP以及胰外器官損傷情況進行系統(tǒng)性研究，降低臨床FAP死亡率，有極其重要的意義。本實驗擬用L-精氨酸(L-arginine，L-Arg)作為AP誘導(dǎo)劑，通過新的注射方式來探討大鼠FAP模型的建立。 方法：144只雄性Wistar大鼠，隨機分為實驗組(L-Arg組，n=72)和對照組(NS組，n=72)。L-Arg組：20％L-精氨酸先從腹腔內(nèi)注射(450mg／100g大鼠體重)一次，間隔2h后，再從皮下注射(150mg／100g大鼠體重)一次。NS組：以同樣的方式注射等體積的生理鹽水。兩組于末次注射后各取30只大鼠，觀察0h—120h內(nèi)大鼠病死率；在0、6、12、24、48、72和120h各相應(yīng)時點各取6只大鼠處死，觀察大鼠血清淀粉酶、脂肪酶、胰腺濕／干比率、血常規(guī)、肝功能、腎功能、肺功能的變化；應(yīng)用HE染色觀察胰腺、肝臟、腎臟、肺臟在以上各時點的病理改變，并給予病理評分或評級。 結(jié)果：L-Arg組和NS組分別經(jīng)兩次間隔2h于腹腔和皮下注射藥物后： (1)L-Arg組30只大鼠，0h～6h死亡0只，6～12h死亡1只，12～24h死亡2只，24～48h死亡7只，48～72h死亡5只，72～120h死亡1只，總死亡率為16／30=53.33％。NS組30只大鼠120h內(nèi)無死亡。 (2)L-Arg組血清淀粉酶活性6h開始增高，24h達到高峰，6、12、24、48h血清淀粉酶活性與NS組比較差異有顯著(P＜0.01)； (3)L-Arg組血清脂肪酶活性6h顯著升高，12h達峰值，6h以后其活性與NS組比較差異均有統(tǒng)計意義(P＜0.01)； (4)L-Arg組胰腺濕／干比率6h升高，48h達峰值，6h以后其活性與NS組比較差異均有統(tǒng)計意義(P＜0.05)；
[Abstract]:Background: as a special type of acute pancreatitis, fulminant acute pancreattis (FAPP) is characterized by rapid onset of acute pancreatitis, severe onset, early multiple organ dysfunction and high mortality.Although the etiology and treatment of this kind of pancreatitis are often reported in clinic at present, there are few researches on FAP and the damage of extrapancreatic organs at home and abroad, and the understanding is extremely limited.Therefore, it is of great significance to establish FAP animal model, and then to systematically study the injury of FAP and extrapancreatic organs, and to reduce the death rate of clinical FAP.In this experiment, L-arginine L-arginine L-Arg was used as the inducer of AP to explore the establishment of rat FAP model by a new injection method.Methods one hundred and forty-four male Wistar rats were randomly divided into two groups: the experimental group (L-Arg group) and the control group (NS group (n = 72). The L-Arg group (n = 20) was injected intraperitoneally with arginine (n = 450 mg / 100 g).Once again, the NS group was injected with the same volume of normal saline in the same way.After the last injection, 30 rats in each group were taken to observe the mortality rate of rats in 0h-120h, 6 rats were killed at the corresponding time points of 0: 12, 24, 4872 and 120 hours, and the serum amylase, lipase, wet / dry ratio of pancreas, blood routine, liver function were observed.The pathological changes of pancreas, liver, kidney and lung were observed by HE staining at each time point above, and the pathological scores or ratings were given.Results the two groups were injected intraperitoneally and subcutaneously after 2 h interval.In the L-Arg group, 30 rats died for 6 hours and 0 rats died for 12 hours. One rat died at 24 hours. 7 rats died for 48 hours and 5 rats died for 72 hours. The total death rate was that 30 rats in 16/30=53.33%.NS group did not die within 120 hours.The activity of serum amylase in L-Arg group began to increase at 6h and reached the peak at 6121224h at 48h. There was a significant difference in serum amylase activity between group A and group NS (P < 0.01).The activity of serum lipase in the L-Arg group was significantly higher than that in the NS group at 6 h and reached the peak at 12 h and reached the peak level at 6 h. The difference was statistically significant compared with that in NS group (P < 0.01).The wet / dry ratio of pancreas in the L-Arg group was significantly higher than that in the NS group (P < 0.05).
【學(xué)位授予單位】：汕頭大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2006
【分類號】：R-332

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