本文選題：B7-H4　切入點：同種異體移植　出處：《第二軍醫(yī)大學(xué)》2007年碩士論文

【摘要】： 本課題利用攜帶免疫負調(diào)控分子B7-H4的重組質(zhì)粒轉(zhuǎn)染自BABL/c小鼠分離得到的胰島細胞,并采用腎包膜下注射的方法將其移植給誘發(fā)Ⅰ型糖尿病的C57BL/6小鼠模型,觀察移植物的功能狀態(tài)和存活時間,探索避免同種異體胰島移植排斥反應(yīng)的有效方法。在體外將基因修飾的胰島細胞與T淋巴細胞混合培養(yǎng),觀察其對T細胞增殖和細胞因子分泌的影響。結(jié)果顯示:該法能明顯改善受體鼠糖尿病癥狀,有效控制血糖水平,顯著延長同種異體移植胰島的存活期;B7-H4基因修飾的胰島細胞在體外刺激T淋巴細胞增殖的能力顯著下降,并可明顯抑制IL-2、IFN-γ的分泌。為Ⅰ型糖尿病的胰島移植治療研究提供了新的途徑,為進一步的免疫負向調(diào)控分子的理論和應(yīng)用研究提供了新的依據(jù)和方法。
[Abstract]:In this study, islet cells isolated from BABL/c mice were transfected with recombinant plasmid carrying B7-H4, and transplanted into C57BL/6 mice model of type 1 diabetes mellitus by subcapsular injection.To observe the functional state and survival time of grafts and to explore an effective method to avoid allograft rejection.The effects of gene modified islet cells and T lymphocytes on T cell proliferation and cytokine secretion were observed in vitro.The results showed that this method could significantly improve the symptoms of diabetes in recipient mice, effectively control the level of blood glucose, and prolong the survival period of islet allografts significantly. The ability of stimulating T lymphocyte proliferation of islet cells modified with B7-H4 gene was significantly decreased in vitro.It also inhibited the secretion of IFN- 緯.It provides a new approach for the study of islet transplantation in type I diabetes mellitus, and provides a new basis and method for the further study of the theory and application of immuno-negative regulation molecules.
【學(xué)位授予單位】：第二軍醫(yī)大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2007
【分類號】：R392

【參考文獻】

相關(guān)期刊論文 前3條

1 王寶金,代解杰;糧尿病動物模型的研究進展[J];上海實驗動物科學(xué);2003年02期

2 張程亮;向明;鄒曉蕾;彭佳蓓;;髓源性樹突狀細胞過繼轉(zhuǎn)移誘導(dǎo)1型糖尿病小鼠免疫耐受及其機制[J];世界華人消化雜志;2006年33期

3 孫子林,葛祖愷;糖尿病動物模型及其進展[J];中國糖尿病雜志;1999年04期

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本文編號：1708811