本文選題：登革病毒　切入點(diǎn)：RT-PCR技術(shù)　出處：《第一軍醫(yī)大學(xué)》2006年碩士論文

【摘要】：一、研究背景和目的 登革病毒(Dengue virus，DEN)為黃病毒屬成員，是引起登革熱(DF)和登革出血熱(DHF)／登革休克綜合癥(DSS)的病原體，有4種不同的血清型(1-4型)。主要傳播媒介是埃及伊蚊和白蚊伊蚊。流行于熱帶和亞熱帶的100多個(gè)國家和地區(qū)，我國自1978年以來主要在海南、廣東、廣西、臺(tái)灣和福建等東南沿海地區(qū)發(fā)生。世界衛(wèi)生組織估計(jì)全球每年DF發(fā)病人數(shù)約5千萬到1億，并導(dǎo)致25至50萬DHF病例和2.4萬人死亡。DF已成為全球范圍內(nèi)嚴(yán)重的公共衛(wèi)生問題，因此加強(qiáng)對(duì)登革病毒致病機(jī)理及疫苗研究具有重要的現(xiàn)實(shí)意義。 登革病毒屬黃病毒屬(Flavivirus)，是一類有包膜的單股、正鏈RNA病毒，在復(fù)制過程中不形成DNA中間體，因此要在基因水平研究其特征存在一定的困難，因?yàn)橥蛔儭⒅亟M、克隆等分子生物學(xué)技術(shù)都是在DNA水平上的操作。感染性轉(zhuǎn)錄體技術(shù)提供了解決這一難題的新途徑。該技術(shù)是把病毒RNA逆轉(zhuǎn)錄成cDNA，并置于RNA聚合酶啟動(dòng)子的下游，在RNA聚合酶的作用下體外轉(zhuǎn)錄出全長正鏈RNA，，經(jīng)轉(zhuǎn)染敏感細(xì)胞后復(fù)制出子代病毒顆粒。由于這種子代病毒來自cDNA，研究者就可以在DNA水平上進(jìn)行操作，從而達(dá)到人為改造病毒，研究其復(fù)制、表達(dá)與致病機(jī)理的目的。 本研究選擇登革2型病毒(Dengue 2 virus，DEN2)NGC株進(jìn)行感染性轉(zhuǎn)
[Abstract]:I. background and purpose of the study. Dengue virus (DEN), a member of the genus Flavovirus, is the cause of dengue fever (DFD) and dengue haemorrhagic fever (DHFF / DSS). There are four different serotypes of Aedes aegypti and Aedes albopictus. Aedes aegypti and Aedes albopictus are prevalent in more than 100 countries and regions in the tropics and subtropics. China has mainly been in Hainan, Guangdong, Guangxi since 1978. Southeast coastal areas such as Taiwan and Fujian. The World Health Organization estimates that about 50 to 100 million people worldwide suffer from DF every year, causing 25 to 500000 DHF cases and 24000 deaths. DF has become a serious public health problem worldwide. Therefore, it is of great practical significance to strengthen the research on the pathogenesis and vaccine of dengue virus. Dengue virus belongs to Flavivirus, a class of single-stranded, positive stranded RNA virus with envelope, which does not form DNA intermediates during replication, so it is difficult to study its characteristics at the gene level because of mutation and recombination. Molecular biological techniques such as cloning are operated at the DNA level. Infectious transcriptome techniques provide a new way to solve this problem. This technique is to reverse the viral RNA into cDNAs and place them downstream of the RNA polymerase promoter. Under the action of RNA polymerase, full-length positive RNAs are transcribed in vitro, and then transferred into sensitive cells to replicate the offspring virus particles. Because the progeny virus comes from DNA, the researchers can manipulate it at the DNA level, so that the virus can be artificially modified. To study its replication, expression and pathogenesis. In this study, we selected dengue 2 virus Dengue 2 virus DEN2NGC strain for infectious transformation.
【學(xué)位授予單位】：第一軍醫(yī)大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2006
【分類號(hào)】：R373

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