本文選題：HIF-1α　切入點：VEGF　出處：《蘇州大學》2006年碩士論文

【摘要】： 目的:從小鼠體內(nèi)和體外兩方面,探討LPS誘導的急性炎癥組織中和體外培養(yǎng)的巨噬細胞內(nèi)HIF-1α表達的改變及其意義 方法:將Balb/c小鼠分為正常對照組及炎癥組,炎癥組采用卡介苗(BCG)配合脂多糖(LPS)尾靜脈注射法建立小鼠急性全身性炎癥損傷模型。取出肝臟和肺臟進行常規(guī)石蠟切片、HE染色觀察臟器的病理改變,免疫組織化學SP法觀察HIF-1α及VEGF在組織中的表達改變。另取正常Balb/c小鼠,分離腹腔巨噬細胞分為正常對照組、LPS刺激組,刺激組給予LPS、對照組給予PBS作用10小時后,采用RT-PCR法半定量檢測HIF-1α、VEGF基因mRNA的表達水平,應用細胞免疫化學染色法檢測巨噬細胞HIF-1α蛋白的表達水平。 結(jié)果:炎癥組小鼠肝臟及肺組織HIF-1α、VEGF表達較正常對照組顯著增加(p均0.01)。體外培養(yǎng)中,與正常對照組比較,LPS刺激的巨噬細胞HIF-1α基因表達無明顯改變(P0.05),但HIF-1α蛋白表達顯著增加且VEGF基因表達上調(diào)(p均0.01)。 結(jié)論:由LPS引起小鼠全身急性炎性時,肝和肺組織表達的HIF-1α及VEGF蛋白水平升高;體外常氧條件下,LPS刺激的小鼠巨噬細胞可在轉(zhuǎn)錄后水平上調(diào)HIF-1α蛋白質(zhì)的表達,并可上調(diào)靶基因VEGF的表達;提示常氧條件下,LPS可通過轉(zhuǎn)錄后水平上調(diào)HIF-1α蛋白的表達從而參與體內(nèi)的急性炎癥反應。
[Abstract]:Objective: to investigate the changes and significance of HIF-1 偽 expression in acute inflammatory tissue induced by LPS and macrophages cultured in vitro and in vivo in mice. Methods: Balb/c mice were divided into normal control group and inflammatory group. The acute systemic inflammatory injury model of mice was established by BCG combined with lipopolysaccharide (LPS) injection into tail vein. The liver and lung were taken out for routine paraffin section and HE staining to observe the pathological changes of viscera. Immunohistochemical SP method was used to observe the expression of HIF-1 偽 and VEGF in normal Balb/c mice. The peritoneal macrophages were isolated and divided into two groups: the normal control group was stimulated by PBS, and the control group was treated with PBS for 10 hours. The expression of HIF-1 偽 -VEGF gene mRNA was detected by semi-quantitative RT-PCR method, and the expression of HIF-1 偽 protein in macrophages was detected by cytoimmunochemical staining. Results: compared with the normal control group, the expression of HIF-1 偽 -VEGF in the liver and lung tissues of the inflammatory group increased significantly. The expression of HIF-1 偽 gene in LPS-stimulated macrophages was not significantly changed, but the expression of HIF-1 偽 protein was significantly increased and the expression of VEGF gene was up-regulated. Conclusion: the expression of HIF-1 偽 and VEGF protein in liver and lung tissues increased during acute inflammation induced by LPS, and the expression of HIF-1 偽 protein was up-regulated by LPS-stimulated mouse macrophages at posttranscriptional level. The expression of target gene VEGF could be upregulated, which suggested that lipopolysaccharide could up-regulate the expression of HIF-1 偽 protein at posttranscriptional level and participate in the acute inflammatory response in vivo.
【學位授予單位】：蘇州大學
【學位級別】：碩士
【學位授予年份】：2006
【分類號】：R363

【參考文獻】

相關期刊論文 前4條

1 許春梅,董衛(wèi)國,余保平,羅和生,于皆平;低氧誘導因子-1α及誘導型一氧化氮合酶在炎癥性腸病中的表達[J];第四軍醫(yī)大學學報;2004年17期

2 劉金波,丁俊清,王莉;炎癥牙髓中缺氧誘導因子-1α的免疫組化研究[J];臨床口腔醫(yī)學雜志;2003年02期

3 許春梅,董衛(wèi)國,余保平,羅和生,于皆平;炎癥性腸病中低氧誘導因子-1α表達的研究[J];胃腸病學和肝病學雜志;2004年03期

4 謝可鳴,茆勇,謝平,顧永平;當歸、阿魏酸鈉對小鼠炎性肝損傷的抑制效應及其與ICAM-1和E-selectin表達的關系研究[J];中國病理生理雜志;2004年12期

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