本文選題：汞　切入點(diǎn)：�；撬徜\　出處：《天津醫(yī)科大學(xué)》2006年碩士論文

【摘要】：目的 汞是強(qiáng)神經(jīng)毒物,牛磺酸和鋅對(duì)腦發(fā)育有重要調(diào)節(jié)作用。本實(shí)驗(yàn)通過(guò)觀測(cè)補(bǔ)充�；撬徜\(TZC)對(duì)染Hg大鼠皮層和海馬一氧化氮合酶(NOS)活力、神經(jīng)元型一氧化氮合酶(nNOS)蛋白和基因表達(dá)的影響,以及觀察在體外培養(yǎng)皮層神經(jīng)元時(shí),TZC對(duì)染Hg大鼠神經(jīng)元生長(zhǎng)活力及突起長(zhǎng)度的影響,研究TZC拮抗Hg對(duì)神經(jīng)系統(tǒng)損害的機(jī)理。 方法 選用雄性健康(出生后21d)Wistar大鼠60只,體重(2004±20)g,隨機(jī)分為對(duì)照組、染汞組、汞低�；撬徜\組和汞高牛磺酸鋅組;分別飲用蒸餾水,4.3mg/kg·d HgCl_2飲水,4.3mg/kg·d HgCl_2飲水和0.23 g/L TZC飲水,4.3mg/kg·d HgCl_2飲水和0.46g/L TZC飲水,每組15只大鼠。喂養(yǎng)75天時(shí),用Y-迷宮實(shí)驗(yàn)測(cè)試大鼠學(xué)習(xí)記憶能力;喂養(yǎng)90天時(shí),用NADPH-d組織化學(xué)、nNOS免疫組織化學(xué)方法分別測(cè)定大鼠海馬NOS活力、nNOS蛋白和基因表達(dá)。 成年雄性健康昆明系小鼠20只,體重(20±2.0)g,隨機(jī)分為對(duì)照組、小劑量氯化汞組、更小劑量氯化汞組和小劑量氯化汞+牛磺酸鋅組;每組5只,隔天一次對(duì)小鼠進(jìn)行腹腔注射HgCl_2(0.1mg/mL,0.05mg/mL),按10ml/kg體重注射:4小時(shí)后注射等劑量的TZC(100mg/mL)。10天后取小鼠腦組織,用RT-PCR測(cè)定nNOS的基因表達(dá)水平。 通過(guò)建立皮層神經(jīng)元原代培養(yǎng)實(shí)驗(yàn)技術(shù),觀察不同濃度TZC(0.25×10~-3mg/ml、0.5×10~-3mg/ml、1.0×10~-3mg/ml、2.0×10~-3mg/ml、4.0×10~-3mg/ml)和不同劑量HgCl_2(8×10~-6mg/ml、8×10~-5mg/ml、8×10~-4mg/ml、8×10~-3mg/ml、8×10~-2mg/ml)對(duì)培養(yǎng)皮層神經(jīng)元生長(zhǎng)活力及突起長(zhǎng)度的影響。 結(jié)果 1.行為學(xué)測(cè)試結(jié)果 染汞75天后,與對(duì)照組大鼠相比,單純?nèi)竟M大鼠Y-迷宮實(shí)驗(yàn)學(xué)會(huì)次數(shù)明顯增多(P0.05)。與單純?nèi)竟M相比,給予高TZC干預(yù)的染汞組大鼠Y-迷宮實(shí)驗(yàn)學(xué)會(huì)次數(shù)明顯減少(P0.05)。
[Abstract]:Objective Mercury is a strong neurotoxin, taurine and zinc play an important role in regulating brain development. The activities of nitric oxide synthase (NOS) in cortex and hippocampus of rats exposed to Hg were observed by supplementation of TZC with zinc taurine. The effects of neuronal nitric oxide synthase (nNOS) protein and gene expression, and the effects of TZC on the growth activity and neurite length of neurons in cultured cortical neurons in vitro were observed, and the mechanism of TZC antagonistic effect of Hg on nervous system damage was studied. Methods 60 healthy male 21d)Wistar rats were randomly divided into control group, low taurine zinc group and high taurine zinc mercury group. Drinking distilled water 4.3mg / kg d HgCl_2 drinking water 4.3mg / kg d HgCl_2 drinking water and 0.23g / L TZC drinking water drinking water 4.3mg / kg d HgCl_2 drinking water and 0.46g/L TZC drinking water respectively. After 75 days of feeding, the learning and memory abilities of rats were tested by Ymaze test, and 90 days after feeding, NADPH-d histochemistry and nNOS immunohistochemical method were used to detect the activity of NOS protein and gene expression in hippocampus of rats. Twenty adult male healthy Kunming mice were randomly divided into control group, low dose mercuric chloride group, lower dose mercuric chloride group and small dose mercuric acid taurine zinc group, with 5 rats in each group. Mice were injected intraperitoneally with 0.1 mg / mL HgCl _ 2C _ (0.1) mg 路m ~ (-1) 路L ~ (-1) and 0.05 mg 路m ~ (-1) 路mL ~ (-1) by intraperitoneal injection the next day. The brain tissues of mice were taken after injection of the same dose of TZC(100mg/mL).10 4 hours later according to the weight of 10ml/kg, and the expression of nNOS gene was determined by RT-PCR. By establishing the primary culture technique of cortical neurons, the effects of different concentrations of TZC(0.25 脳 10 ~ (-3) mg 路ml ~ (-1) ~ (0.5 脳 10 ~ (-3) mg 路ml ~ (-1)) 1.0 脳 10 ~ (-3) mg 路ml ~ (-1) 路ml ~ (-1) ~ (2) 脳 10 ~ (-3) mg 路ml ~ (-1) and different doses of HgCl_2(8 脳 10 ~ (-3) ~ (-3) mg 路ml ~ (-1) ~ (8 脳 10 ~ (-10) ~ (-3) mg 路ml ~ (-1) ~ (-1)) on the growth activity and the length of neuronal protuberance were observed. Results. 1. After 75 days of behavior test, compared with the control group, the number of Y- labyrinth experimental societies of rats exposed to mercury alone increased significantly (P 0.05), and compared with that of the control group, there was a significant increase in the number of Y- labyrinth experimental societies of rats exposed to mercury alone. The number of Y- labyrinth experiment in mercury exposed group treated with high TZC significantly decreased P0. 05.
【學(xué)位授予單位】：天津醫(yī)科大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2006
【分類(lèi)號(hào)】：R363

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