本文選題：DPPII　切入點：DPPIII　出處：《吉林大學》2007年碩士論文

【摘要】： 目的:探討DPPII及DPPIII在正常大鼠眼球各組織中的免疫表達。 方法:選取正常兩性Wistar大鼠,共40只,分為2組,將大鼠處死后取出眼球,放入10%甲醛固定,石蠟包埋,用切片機切取4μm厚切片。應(yīng)用抗生物素蛋白-生物素-過氧化物酶復(fù)合體法檢測外切肽酶DPPII、DPPIII在正常大鼠眼球各組織中的免疫表達。 結(jié)果:發(fā)現(xiàn)DPPII在角膜上皮細胞、角膜基質(zhì)層、角膜內(nèi)皮細胞、睫狀體無色素上皮細胞、虹膜色素上皮細胞、晶狀體上皮細胞、晶狀體纖維、視網(wǎng)膜神經(jīng)纖維層、神經(jīng)節(jié)細胞、視網(wǎng)膜色素上皮細胞免疫染色呈陽性;DPPIII在角膜上皮細胞、角膜內(nèi)皮細胞、睫狀體無色素上皮細胞、虹膜色素上皮細胞、晶狀體上皮細胞、晶狀體纖維、視網(wǎng)膜神經(jīng)纖維層、神經(jīng)節(jié)細胞、視網(wǎng)膜色素上皮細胞免疫染色呈陽性。 結(jié)論:DPPII主要存在于角膜上皮細胞、角膜基質(zhì)層、角膜內(nèi)皮細胞、睫狀體無色素上皮細胞、虹膜色素上皮細胞、晶狀體上皮細胞、晶狀體纖維、視網(wǎng)膜神經(jīng)纖維層、神經(jīng)節(jié)細胞、視網(wǎng)膜色素上皮細胞;DPPIII主要存在于角膜上皮細胞、角膜內(nèi)皮細胞、睫狀體無色素上皮細胞、虹膜色素上皮細胞、晶狀體上皮細胞、晶狀體纖維、視網(wǎng)膜神經(jīng)纖維層、神經(jīng)節(jié)細胞、視網(wǎng)膜色素上皮細胞。
[Abstract]:Objective: to investigate the expression of DPPII and DPPIII in normal rat eyeball tissues. Methods: a total of 40 normal Wistar rats were divided into two groups. After the rats were killed, the eyeballs were removed, fixed with 10% formaldehyde, and embedded in paraffin. Four 渭 m thick sections were cut by slicing machine, and the immunological expression of exopeptidase DPPIIPIII in normal rat eyeball was detected by the method of biotin-biotin peroxidase complex. Results: DPPII was found in corneal epithelial cells, corneal stromal layer, corneal endothelial cells, ciliary body non-pigmented epithelial cells, iris pigment epithelial cells, lens epithelial cells, lens fibers, retinal nerve fiber layer, ganglion cells. Retinal pigment epithelial cells (RPE) showed positive staining for DPPIII in corneal epithelial cells, corneal endothelial cells, ciliary unpigmented epithelial cells, iris pigment epithelial cells, lens epithelial cells, lens fibers, retinal nerve fiber layer, The ganglion cells and retinal pigment epithelium cells were immunoreactive. ConclusionTwo DPPII mainly exists in corneal epithelial cells, corneal stromal layer, corneal endothelial cells, ciliary unpigmented epithelial cells, iris pigment epithelial cells, lens epithelial cells, lens fibers and retinal nerve fiber layers. DPPIII mainly exists in corneal epithelial cells, corneal endothelial cells, ciliary body non-pigmented epithelial cells, iris pigment epithelial cells, lens epithelial cells, lens fibers. Retinal nerve fiber layer, ganglion cells, retinal pigment epithelial cells.
【學位授予單位】：吉林大學
【學位級別】：碩士
【學位授予年份】：2007
【分類號】：R361.2

【參考文獻】

相關(guān)期刊論文 前1條

1 張輝,王陸飛,肖俊;Dipeptidyl peptidase Ⅲ在先天性白內(nèi)障大鼠晶狀體中的免疫活性變化[J];眼科新進展;2003年06期

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