本文選題：巨細(xì)胞病毒　切入點(diǎn)：粒單系祖細(xì)胞　出處：《瀘州醫(yī)學(xué)院》2006年碩士論文

【摘要】：目的:本課題主要探討人類造血干祖細(xì)胞(Hematopoietic Stem-Progenitor Cell,HSPC)向粒單系(Colony Fortming Unit-Granulocyte-macrophage,CFU-GM)增殖過(guò)程中HOXB6,HOXA5基因表達(dá)的情況,并且用人類巨細(xì)胞病毒(Human Cytomegalovirus,HCMV)和/或全反式維甲酸(all-trans-retinoticacid,ATRA)進(jìn)行干擾,以探討HCMV、ATRA在此過(guò)程中對(duì)HOXB6、HOXA5基因的影響,以進(jìn)一步明確HCMV抑制臍血粒單系祖細(xì)胞增殖的機(jī)制,以及ATRA能有效治療粒系白血病,尤其是急性早幼粒細(xì)胞白血病的可能機(jī)制。方法:1.臍血標(biāo)本由本院產(chǎn)房提供,取自正常足月順產(chǎn)新生兒斷臍后的胎盤(pán)段臍血。2.實(shí)驗(yàn)分組。實(shí)驗(yàn)分4組:(1)空白對(duì)照組:不加病毒液,代之等量IMDM加入基本培養(yǎng)體系。(2)HCMV組:HCMV—AD_(169)滴度為組織培養(yǎng)半數(shù)感染量(tissue culture infectious,TCID_(50))10~(-4.5)/0.1ml,按100TCID_(50) 0.1ml感染培養(yǎng)體系。(3)ATRA組:加入ATRA稀釋液0.1ml,終濃度60nmol/(?)l。(4)HCMV+ATRA組:同時(shí)加入HCMV—AD_(169)病毒稀釋液0.1ml及ATRA0.1ml,終濃度同上。3.采用造血祖細(xì)胞體外培養(yǎng)技術(shù),以HCMV—AD_(169)和/或ATRA持續(xù)干擾人類造血干祖細(xì)胞,觀察正常組、HCMV—AD_(169)組、ATRA組、以及ATRA+HCMV組的人類造血干祖細(xì)胞經(jīng)人粒細(xì)胞巨噬細(xì)胞集落刺激因子(Granulocyte-macrophage colony-stimulating,GM-CSF)誘導(dǎo)
[Abstract]:Objective: to explore human hematopoietic stem progenitor cells. Colony Fortming of Stem-Progenitor Cell-HSPCs. The expression of HOXB6 / HOXA5 gene during the proliferation of Unit-Granulocyte-macrophaget-macrophage (CFU-GM) was investigated by using human cytomegalovirus (human cytomegalovirus) and / or all-trans retinoic acid all-trans-retinoic acid (ATRAA) to investigate the effect of HCMVATRA on HOXB6 HOXA5 gene. To further clarify the mechanism of HCMV inhibiting the proliferation of cord blood granulocyte progenitor cells and the possible mechanism that ATRA can effectively treat myeloid leukemia, especially acute promyelocytic leukemia. Umbilical cord blood of placental segment taken from normal full-term spontaneous delivery newborns after umbilical cord breakage. 2. The experiment was divided into 4 groups: control group (control group): no virus solution, Instead of adding the same amount of IMDM to the basic culture system. 1: HCMV-ADS169) titer of tissue culture Infectious culture Infectious culture 10 ~ (-4. 5)% -0.1 ml, according to 100 TCT + + 50) 0.1ml infection culture system .3ATRA group: add 0.1 ml ATRA diluent, final concentration 60 nmol /??? HCMV ATRA group: HCMV-ADS169) virus diluent 0.1ml and ATRA 0.1ml were added simultaneously, the final concentration was same as .3.The hematopoietic progenitor cell culture technique was used to continuously interfere with human hematopoietic stem progenitor cells by HCMV-ADS-169) and / or ATRA, and to observe the normal group HCMV-ADstruc-1699) group, and to observe the effect of HCMV-ADS1699) on human hematopoietic stem progenitor cells, and to observe the effect of HCMV-ADS1699 on human hematopoietic stem progenitor cells. Human hematopoietic stem progenitor cells in ATRA HCMV group were induced by granulocyte-macrophage colony-stimulating factor GM-CSF.
【學(xué)位授予單位】：瀘州醫(yī)學(xué)院
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2006
【分類號(hào)】：R363

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