本文選題：酸性肽　切入點(diǎn)：谷氨酸　出處：《鄭州大學(xué)》2005年碩士論文　論文類(lèi)型：學(xué)位論文

【摘要】：目的:鑒定酸性肽(acidic peptide,AP)在動(dòng)物腦中是否普遍存在,測(cè)定大鼠腦內(nèi)酸性肽和谷氨酸(glutamic acid,Glu)的含量,并探討外源性酸性肽對(duì)大鼠內(nèi)源性酸性肽及谷氨酸含量的影響。 方法:用紙電泳法(300V,1h)分離定位marker,此marker包括谷氨酸、酸性肽和谷胱甘肽三種標(biāo)準(zhǔn)小肽或氨基酸,然后用此marker鑒別牛、羊、雞、鴨、兔、大鼠等多種動(dòng)物腦中AP的存在。用標(biāo)準(zhǔn)谷氨酸、酸性肽和谷胱甘肽配成濃度分別為0.5mg/ml、1.0mg/ml、2.0mg/ml、3.0mg/ml、4.0mg/ml的混合液,每一濃度各取4μl點(diǎn)樣,然后進(jìn)行電泳分離,在烘箱中烘10min,剪下斑點(diǎn),洗脫液洗脫,分光光度法測(cè)定不同濃度的AP和Glu的吸光度值(OD)。重復(fù)10次實(shí)驗(yàn)。以平均OD值為縱坐標(biāo),濃度或含量為橫坐標(biāo),繪制AP和Glu的標(biāo)準(zhǔn)曲線(xiàn)。將大鼠隨機(jī)分為6組(每組10只),其中1組為對(duì)照組,其余5組按100mg/kg酸性肽灌胃,分別在灌胃后1h、
[Abstract]:Aim: to determine the prevalence of acidic peptide (APD) in animal brain, to determine the contents of acidic peptide and glutamic acid acid acid (glutamic acid acid) in rat brain, and to investigate the effect of exogenous acidic peptide on the content of endogenous acid peptide and glutamate in rats. Methods: Marker was isolated by paper electrophoresis for 1 h. The marker consisted of glutamic acid, acid peptide and glutathione. The marker was used to identify cattle, sheep, chicken, duck and rabbit. The presence of AP in the brains of rats and other animals. A mixture of 0.5 mg / ml of standard glutamate, acidic peptide and glutathione was prepared with a concentration of 1.0 mg / ml of 1.0 mg / ml / ml of 1.0 mg / ml / ml of 2.0 mg / ml / ml of 2.0 mg / ml / ml / ml of 4.0 mg / ml / ml respectively, and 4 渭 l / ml of each concentration was taken for each concentration, then separated by electrophoretic electrophoresis, dried in the oven for 10 minutes, and the spots were cut off, The absorbance values of AP and Glu of different concentrations were determined by spectrophotometry. Ten experiments were repeated. The average OD value was taken as the vertical coordinate and the concentration or content as the horizontal coordinate. The standard curves of AP and Glu were drawn. The rats were randomly divided into 6 groups (10 rats in each group, 1 group as control group, the other 5 groups were fed with 100mg/kg acid peptide at 1 hour after gavage, respectively).
【學(xué)位授予單位】：鄭州大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2005
【分類(lèi)號(hào)】：Q593.2

【參考文獻(xiàn)】

相關(guān)期刊論文 前10條

1 孫炳偉;腸上皮細(xì)胞二肽轉(zhuǎn)運(yùn)載體的生物學(xué)功能及研究進(jìn)展[J];腸外與腸內(nèi)營(yíng)養(yǎng);2003年01期

2 韓興泰;動(dòng)物消化道內(nèi)肽的吸收與體內(nèi)肽的代謝[J];中國(guó)草食動(dòng)物;1999年02期

3 晏向華,翟明仁,黎觀(guān)紅,林媛媛;動(dòng)物營(yíng)養(yǎng)中小肽的營(yíng)養(yǎng)研究新進(jìn)展[J];廣東飼料;2000年03期

4 梁振,張維冰,張玉奎;CZE-ESI-MS聯(lián)用測(cè)定小肽混合物的研究[J];高等學(xué)�；瘜W(xué)學(xué)報(bào);2004年03期

5 劉東梅;用分光光度法繪制標(biāo)準(zhǔn)曲線(xiàn)時(shí)應(yīng)注意的問(wèn)題[J];甘肅環(huán)境研究與監(jiān)測(cè);1996年04期

6 安玉會(huì),郭茂峰,孟慶瑞,張維娟,李懷洲,王志超,章萍;腦肽精對(duì)阿爾茨海默病模型大鼠的治療作用[J];鄭州大學(xué)學(xué)報(bào)(醫(yī)學(xué)版);2003年06期

7 安玉會(huì),劉莊勵(lì),趙建軍,王占國(guó),鄭香梅,章萍,王建軍;二種牛神經(jīng)肽的分離[J];河南醫(yī)科大學(xué)學(xué)報(bào);1998年04期

8 安玉會(huì),劉莊勵(lì),趙建軍,王占國(guó),鄭香梅,章萍,王建軍;酸性牛神經(jīng)肽1的抗嗎啡作用和成癮性研究[J];河南醫(yī)科大學(xué)學(xué)報(bào);1998年04期

9 王志超,安玉會(huì);生物活性肽的研究進(jìn)展[J];河南醫(yī)學(xué)研究;2004年04期

10 孫忠保,閻宏;動(dòng)物小肽營(yíng)養(yǎng)研究新進(jìn)展[J];寧夏農(nóng)學(xué)院學(xué)報(bào);2004年02期

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