本文選題：骨髓基質(zhì)干細胞　切入點：細胞分化　出處：《廣西醫(yī)科大學(xué)》2006年碩士論文　論文類型：學(xué)位論文

【摘要】： 目的:探討體外分離及擴增純化兔骨髓基質(zhì)干細胞的實驗方法,并研究其生物學(xué)特性;以不同誘導(dǎo)液在體外對BMSCs誘導(dǎo)分化為雪旺樣細胞,探討以BMSCs作為周圍神經(jīng)組織工程種子細胞的可行性。 方法:采用Percoll(密度為1.073g/ml)分離液和貼壁篩選法,從兔股骨中分離BMSCs進行純化、培養(yǎng);原代細胞按1: 2比例進行傳代培養(yǎng);在DMEM-LG + 10%FBS中培養(yǎng),用β-ME、b-FGF和黃芪等為誘導(dǎo)劑對第1, 3, 5代骨髓基質(zhì)干細胞進行定向誘導(dǎo)分化,倒置像差顯微鏡觀察細胞形態(tài)學(xué)化,免疫細胞化學(xué)鑒定S-100蛋白和GFAP的表達率。 結(jié)果:原代及傳代培養(yǎng)顯示,兔BMSCs具有活躍的增殖能力,第2, 3, 5代細胞的生長曲基本相同;誘導(dǎo)后的兔骨髓基質(zhì)干細胞具有類似雪旺細胞的形態(tài),免疫細胞化學(xué)鑒定顯示S-100蛋白和GFAP的表達率(76±6.7)%、(25±5.45)%。 結(jié)論:應(yīng)用本實驗方法培養(yǎng)兔髓基質(zhì)干細胞,體外生長穩(wěn)定、增殖速度快、可連續(xù)傳代;采用β-ME、b-FGF和黃芪等為誘導(dǎo)劑,可使兔骨髓基質(zhì)干細胞在體外向雪旺樣細胞分化。
[Abstract]:Objective: to investigate the methods of isolating and purifying rabbit bone marrow stromal cells in vitro and their biological characteristics, and to differentiate Schwann like cells into Schwann like cells induced by different inducers in vitro. To explore the feasibility of using BMSCs as seed cells for peripheral nerve tissue engineering. Methods: BMSCs isolated from rabbit femur was purified and cultured by Percollus (density 1.073 g / ml) and adherent screening method, primary cells were subcultured in 1: 2 ratio, and cultured in DMEM-LG 10s. Bone marrow stromal stem cells (BMSCs) of the first, third and fifth passages were induced to differentiate by 尾 -ME-b-FGF and astragalus membranaceus. The morphologic changes of bone marrow stromal cells were observed by inverted aberration microscope. The expression rates of S-100 protein and GFAP were identified by immunocytochemistry. Results: primary culture and passage culture showed that rabbit BMSCs had active proliferative ability, and the growth curve of the 2nd, 3rd and 5th passage cells was basically the same, and the induced rabbit bone marrow stromal stem cells had the morphology similar to Schwann cells, and the growth curve of rabbit bone marrow stromal cells was similar to that of Schwann cells. The expression rate of S-100 protein and GFAP was 76 鹵6.7% and 25 鹵5.45% by immunocytochemistry. Conclusion: rabbit marrow stromal stem cells can be cultured in vitro with stable growth, rapid proliferation and continuous passage, and 尾 -ME-b-FGF and Astragalus membranaceus can induce the differentiation of rabbit bone marrow stromal cells into Schwan-like cells in vitro.
【學(xué)位授予單位】：廣西醫(yī)科大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2006
【分類號】：R329.2

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本文編號：1647344