本文選題：骨髓間充質干細胞　切入點：DAPI　出處：《重慶醫(yī)科大學》2006年碩士論文　論文類型：學位論文

【摘要】： 目的骨髓間充質干細胞(Bone marrow mesenchymal stem cells, Bm-MSCs)具有高度增殖、自我更新能力,可向三個胚層的細胞分化[1],在機體組織損傷修復過程及細胞移植治療等多個領域有廣闊的應用前景。目前,已有成功誘導Bm-MSCs分化為成骨細胞、軟骨細胞、脂肪細胞、神經(jīng)細胞、肝細胞、皮膚上皮細胞等的報道[2~7],尚未見腸壁結締組織影響B(tài)m-MSCs分化的研究。本實驗擬①分離、培養(yǎng)、擴增胎兒Bm-MSCs,觀察其生物學特性;②制備胎兒腸壁結締組織,遴選體外培養(yǎng)時間;③觀察胎兒腸壁結締組織能否誘導Bm-MSCs分化形成上皮細胞?EGF在胎兒腸壁結締組織誘導Bm-MSCs分化中的作用。為Bm-MSCs在消化管組織工程以及腸粘膜損傷性疾病治療研究中的應用,提供基礎理論和實驗依據(jù)。 第一部分 1材料與方法 1.1收集6例4~5個月引產(chǎn)胎兒的四肢骨髓,分離、培養(yǎng)、擴增獲取Bm-MSCs。檢測Bm-MSCs的貼壁率、生長曲線;流式細胞儀檢測CD29、CD71、CD34及HLA-DR的表達,以及細胞周期分析;細胞化學染色檢測非特異性酯酶、堿性磷酸酶及糖原。 1.2 DAPI標記Bm-MSCs,不同時點熒光顯微鏡觀察,計算標記率。
[Abstract]:Objective Bone marrow mesenchymal stem cells (Bm-MSCs) are highly proliferative, self-renewing, and can differentiate into three layers of cells. Bm-MSCs can be widely used in many fields, such as tissue damage repair and cell transplantation therapy, etc. At present, bone marrow mesenchymal stem cells (Bm-MSCs) can differentiate into three layers of cells. Bm-MSCs was successfully induced to differentiate into osteoblasts, chondrocytes, adipocytes, nerve cells, hepatocytes, skin epithelial cells and so on. Fetal Bm-MSCswere amplified to observe the biological characteristics of fetal intestinal wall connective tissue was prepared. The selection of culture time in vitro to observe whether fetal intestinal wall connective tissue can induce Bm-MSCs differentiation into epithelial cells. The role of EGF in the differentiation of Bm-MSCs induced by connective tissue of fetal intestinal wall provides theoretical and experimental basis for the application of Bm-MSCs in the study of digestive tract tissue engineering and the treatment of intestinal mucosal injury diseases. Part one. Materials and methods. 1.1 Bm-MSCswere collected from bone marrow of 6 fetuses of 4 ~ 5 months of induced labor. The adherent rate and growth curve of Bm-MSCs were detected by Bm-MSCs.The expression of CD29, CD71, CD34 and HLA-DR, and cell cycle analysis were detected by flow cytometry. Nonspecific esterase, alkaline phosphatase and glycogen were detected by cytochemical staining. 1. 2 DAPI labeled Bm-MSCs, fluorescence microscope at different time points, the labeling rate was calculated.
【學位授予單位】：重慶醫(yī)科大學
【學位級別】：碩士
【學位授予年份】：2006
【分類號】：R321

【引證文獻】

相關期刊論文 前1條

1 宋祥福;孫冰雪;張靜春;龔守良;;同種異體大鼠骨髓間充質干細胞移植對放射性空腸損傷的修復作用[J];中國比較醫(yī)學雜志;2011年08期

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